The non-dosage compensated Lsp1α gene of Drosophila melanogaster escapes acetylation by MOF in larval fat body nuclei, but is flanked by two dosage compensated genes

<p>Abstract</p> <p>Background</p> <p>In <it>Drosophila melanogaster </it>dosage compensation of most X-linked genes is mediated by the male-specific lethal (MSL) complex, which includes MOF. MOF acetylates histone H4 at lysine 16 (H4K16ac). The X-linked <it>Larval serum protein one </it>α (<it>Lsp1</it>α) gene has long been known to be not dosage compensated. Here we have examined possible explanations for why the <it>Lsp1</it>α gene is not dosage compensated.</p> <p>Results</p> <p>Quantitative RNase protection analysis showed that the genes flanking <it>Lsp1</it>α are expressed equally in males and females and confirmed that <it>Lsp1</it>α is not dosage compensated. Unlike control X-linked genes, <it>Lsp1</it>α was not enriched for H4K16ac in the third instar larval fat body, the tissue in which the gene is actively expressed. X-linked <it>Lsp1α promoter-lacZ </it>reporter transgenes are enriched for H4K16ac in third instar larval fat body. An X-linked reporter gene bracketed by <it>Lsp1</it>α flanking regions was dosage compensated. One of the genes flanking <it>Lsp1</it>α is expressed in the same tissue. This gene shows a modest enrichment for H4K16ac but only at the part of the gene most distant from <it>Lsp1</it>α. Phylogenetic analyses of the sequences of the genomes of 12 <it>Drosophila </it>species shows that <it>Lsp1</it>α is only present within the <it>melanogaster </it>subgroup of species.</p> <p>Conclusion</p> <p><it>Lsp1</it>α is not modified by the MSL complex but is in a region of the X chromosome that is regulated by the MSL complex. The high activity or tissue-specificity of the <it>Lsp1</it>α promoter does not prevent regulation by the MSL complex. The regions flanking <it>Lsp1</it>α do not appear to block access by the MSL complex. <it>Lsp1</it>α appears to have recently evolved within the <it>melanogaster </it>subgroup of <it>Drosophila </it>species. The most likely explanation for why <it>Lsp1</it>α is not dosage compensated is that the gene has not evolved a mechanism to independently recruit the MSL complex, possibly because of its recent evolutionary origin, and because there appears to be a low level of bound MSL complex in a nearby gene that is active in the same tissue.</p

Forecasting the Senate vote on the Supreme Court vacancy

This paper forecasts current senators’ votes on Merrick Garland’s nomination to the U.S. Supreme Court, in the unlikely case that a vote actually takes place. The forecasts are necessarily conditional, awaiting measurement of the nominee’s characteristics. Nonetheless, a model that combines parameters estimated from existing data with values of some measurable characteristics of senators—particularly their party affiliations, party loyalty levels, and ideological positions—is sufficient to identify potential swing voters in the Senate. By accounting for a more nuanced and refined understanding of the confirmation process, our model reveals that if President Obama were to nominate almost any nominee (conservative or liberal) today, that nominee would be rejected if a vote was allowed to take place. So why nominate anyone at all? Obama’s hope for a successful confirmation must come from the stochastic component, that is, from outside the traditional decision-making calculus

Interfacial Mechanophore Activation Using Laser-Induced Stress Waves

A new methodology is developed to activate and characterize mechanochemical transformations at a solid interface. Maleimide–anthracene mechanophores covalently anchored at a fused silica–polymer interface are activated using laser-induced stress waves. Spallation-induced mechanophore activation is observed above a threshold activation stress of 149 MPa. The retro [4+2] cycloaddition reaction is confirmed by fluorescence microscopy, XPS, and ToF-SIMS measurements. Control experiments with specimens in which the mechanophore is not covalently attached to the polymer layer exhibit no activation. In contrast to activation in solution or bulk polymers, whereby a proportional increase in mechanophore activity is observed with applied stress, interfacial activation occurs collectively with spallation of the polymer film

The ocean’s role in the transient response of climate to abrupt greenhouse gas forcing

We study the role of the ocean in setting the patterns and timescale of the transient response of the climate to anthropogenic greenhouse gas forcing. A novel framework is set out which involves integration of an ocean-only model in which the anthropogenic temperature signal is forced from the surface by anomalous downwelling heat fluxes and damped at a rate controlled by a ‘climate feedback’ parameter. We observe a broad correspondence between the evolution of the anthropogenic temperature (T[subscript anthro]) in our simplified ocean-only model and that of coupled climate models perturbed by a quadrupling of CO[subscript 2]. This suggests that many of the mechanisms at work in fully coupled models are captured by our idealized ocean-only system. The framework allows us to probe the role of the ocean in delaying warming signals in the Southern Ocean and in the northern North Atlantic, and in amplifying the warming signal in the Arctic. By comparing active and passive temperature-like tracers we assess the degree to which changes in ocean circulation play a role in setting the distribution and evolution of T[subscript anthro]. The background ocean circulation strongly influences the large-scale patterns of ocean heat uptake and storage, such that T[subscript anthro] is governed by an advection/diffusion equation and weakly damped to the atmosphere at a rate set by climate feedbacks. Where warming is sufficiently small, for example in the Southern Ocean, changes in ocean circulation play a secondary role. In other regions, most noticeably in the North Atlantic, changes in ocean circulation induced by T[subscript anthro] are central in shaping the response.United States. National Aeronautics and Space Administration. Modeling, Analysis, and Prediction ProgramMassachusetts Institute of Technology. Joint Program on the Science & Policy of Global ChangeJames S. McDonnell Foundation (Postdoctoral Fellowship

Insulated piggyBac vectors for insect transgenesis

BACKGROUND: Germ-line transformation of insects is now a widely used method for analyzing gene function and for the development of genetically modified strains suitable for pest control programs. The most widely used transposable element for the germ-line transformation of insects is piggyBac. The site of integration of the transgene can influence gene expression due to the effects of nearby transcription enhancers or silent heterochromatic regions. Position effects can be minimized by flanking a transgene with insulator elements. The scs/scs' and gypsy insulators from Drosophila melanogaster as well as the chicken β-globin HS4 insulator function in both Drosophila and mammalian cells. RESULTS: To minimize position effects we have created a set of piggyBac transformation vectors that contain either the scs/scs', gypsy or chicken β-globin HS4 insulators. The vectors contain either fluorescent protein or eye color marker genes and have been successfully used for germ-line transformation of Drosophila melanogaster. A set of the scs/scs' vectors contains the coral reef fluorescent protein marker genes AmCyan, ZsGreen and DsRed that have not been optimized for translation in human cells. These marker genes are controlled by a combined GMR-3xP3 enhancer/promoter that gives particularly strong expression in the eyes. This is also the first report of the use of the ZsGreen and AmCyan reef fluorescent proteins as transformation markers in insects. CONCLUSION: The insulated piggyBac vectors should protect transgenes against position effects and thus facilitate fine control of gene expression in a wide spectrum of insect species. These vectors may also be used for transgenesis in other invertebrate species

Microplastic in Surface Waters of Urban Rivers: Concentration, Sources, and Associated Bacterial Assemblages

The ecological dynamics of microplastic (\u3c5 mm) are well documented in marine ecosystems, but the sources, abundance, and ecological role of microplastic in rivers are unknown and likely to be substantial. Microplastic fibers (e.g., synthetic fabrics) and pellets (e.g., abrasives in personal care products) are abundant in wastewater treatment plant (WWTP) effluent, and can serve as a point source of microplastic in rivers. The buoyancy, hydrophobic surface, and long transport distance of microplastic make it a novel substrate for the selection and dispersal of unique microbial assemblages. We measured microplastic concentration and bacterial assemblage composition on microplastic and natural surfaces upstream and downstream of WWTP effluent sites at nine rivers in Illinois, United States. Microplastic concentration was higher downstream of WWTP effluent outfall sites in all but two rivers. Pellets, fibers, and fragments were the dominant microplastic types, and polymers were identified as polypropylene, polyethylene, and polystyrene. Mean microplastic flux was 1,338,757 pieces per day, although the flux was highly variable among nine sites (min = 15,520 per day, max = 4,721,709 per day). High-throughput sequencing of 16S rRNA genes showed bacterial assemblage composition was significantly different among microplastic, seston, and water column substrates. Microplastic bacterial assemblages had lower taxon richness, diversity, and evenness than those on other substrates, and microplastic selected for taxa that may degrade plastic polymers (e.g., Pseudomonas) and those representing common human intestinal pathogens (e.g., Arcobacter). Effluent from WWTPs in rivers is an important component of the global plastic “life cycle,” and microplastic serves as a novel substrate that selects and transports distinct bacterial assemblages in urban rivers. Rates of microplastic deposition, consumption by stream biota, and the metabolic capacity of microplastic biofilms in rivers are unknown and merit further research

Now the wars are over: The past, present and future of Scottish battlefields

Battlefield archaeology has provided a new way of appreciating historic battlefields. This paper provides a summary of the long history of warfare and conflict in Scotland which has given rise to a large number of battlefield sites. Recent moves to highlight the archaeological importance of these sites, in the form of Historic Scotland’s Battlefields Inventory are discussed, along with some of the problems associated with the preservation and management of these important cultural sites