XMM-Newton observations of the BL Lac MS 0737+7441

We report on the XMM-Newton observations of the BL Lac object MS 0737.9+7441 during the performance verification phase. A simple power-law fit provides an adequate description of the integrated spectrum in the 0.2-10 keV energy band. The photon index is slightly steeper in the EPIC pn data with 2.38+-0.01 compared to the EPIC MOS data (2.28+-0.01). The difference is most probably due to the present uncertainties in the calibration of the EPIC MOS and EPIC pn data sets. We report evidence for intrinsic absorption in the distant BL Lac above the Galactic column N_H,Gal=3.2*10^20 cm^-2 which is N_H,fit(z=0.315)= (2.70+-0.20)*10^20cm^-2 in the EPIC pn data and N_H,fit(z=0.315)= (3.25+-0.25)*10^20cm^-2 in the EPIC MOS data assuming neutral gas and solar abundances. The flux variations are found to be of the order of 10 %.Comment: 4 pages, 4 Figures, accepted for publication in the special A&A Letters issue for XMM-Newto

The Discovery of an Anomalous X-ray Pulsar in the Supernova Remnant Kes 73

We report the discovery of pulsed X-ray emission from the compact source 1E 1841-045, using data obtained with the Advanced Satellite for Cosmology and Astrophysics. The X-ray source is located in the center of the small-diameter supernova remnant (SNR) Kes 73 and is very likely to be the compact stellar-remnant of the supernova which formed Kes 73. The X-rays are pulsed with a period of ~ 11.8 s, and a sinusoidal modulation of roughly 30 %. We interpret this modulation to be the rotation period of an embedded neutron star, and as such would be the longest spin period for an isolated neutron star to-date. This is especially remarkable since the surrounding SNR is very young, at ~ 2000 yr old. We suggest that the observed characteristics of this object are best understood within the framework of a neutron star with an enormous dipolar magnetic field, B ~ 8x10^14 G

Observations of Accreting Pulsars

We summarize five years of continuous monitoring of accretion-powered pulsars with the Burst and Transient Source Experiment (BATSE) on the Compton Gamma Ray Observatory. Our 20-70 keV observations have determined or refined the orbital parameters of 13 binaries, discovered 5 new transient accreting pulsars, measured the pulsed flux history during outbursts of 12 transients (GRO J1744-28, 4U 0115+634, GRO J1750-27, GS 0834-430, 2S 1417-624, GRO J1948+32, EXO 2030+375, GRO J1008-57, A 0535+26, GRO J2058+42, 4U 1145-619 and A 1118-616), and also measured the accretion torque history of during outbursts of 6 of those transients whose orbital parameters were also known. We have also continuously measured the pulsed flux and spin frequency for eight persistently accreting pulsars (Her X-1, Cen X-3, Vela X-1, OAO 1657-415, GX 301-2, 4U 1626-67, 4U 1538-52, and GX 1+4). Because of their continuity and uniformity over a long baseline, BATSE observations have provided new insights into the long-term behavior of accreting magnetic stars. We have found that all accreting pulsars show stochastic variations in their spin frequencies and luminosities, including those displaying secular spin-up or spin-down on long time scales, blurring the conventional distinction between disk-fed and wind-fed binaries. Pulsed flux and accretion torque are strongly correlated in outbursts of transient accreting pulsars, but uncorrelated, or even anticorrelated, in persistent sources.Comment: LaTeX, psfig, 90 pages, 42 figures. To appear in Dec. 1997 ApJS, Vol 113, #

Imaging the boundaries—innovative tools for microscopy of living cells and real-time imaging

Recently, light microscopy moved back into the spotlight, which is mainly due to the development of revolutionary technologies for imaging real-time events in living cells. It is truly fascinating to see enzymes “at work” and optically acquired images certainly help us to understand biological processes better than any abstract measurements. This review aims to point out elegant examples of recent cell-biological imaging applications that have been developed with a chemical approach. The discussed technologies include nanoscale fluorescence microscopy, imaging of model membranes, automated high-throughput microscopy control and analysis, and fluorescent probes with a special focus on visualizing enzyme activity, free radicals, and protein–protein interaction designed for use in living cells

Collagen VII Expression Is Required in Both Keratinocytes and Fibroblasts for Anchoring Fibril Formation in Bilayer Engineered Skin Substitutes

The blistering disease recessive dystrophic epidermolysis bullosa (RDEB) is caused by mutations in the gene encoding collagen VII (COL7), which forms anchoring fibrils that attach the epidermis to the dermis. Cutaneous gene therapy to restore COL7 expression in RDEB patient cells has been proposed, and cultured epithelial autograft containing COL7-modified keratinocytes was previously tested in clinical trials. Because COL7 in normal skin is expressed in both fibroblasts and keratinocytes, cutaneous gene therapy using a bilayer skin substitute may enable faster restoration of anchoring fibrils. Hypothetically, COL7 expression in either dermal fibroblasts or epidermal keratinocytes might be sufficient for functional anchoring fibril formation in a bilayer skin substitute. To test this, engineered skin substitutes (ESS) were prepared using four combinations of normal + RDEB cells: (1) RDEB fibroblasts + RDEB keratinocytes; (2) RDEB fibroblasts + normal keratinocytes; (3) normal fibroblasts + RDEB keratinocytes; and (4) normal fibroblasts + normal keratinocytes. ESS were incubated in vitro for 2 weeks prior to grafting to full-thickness wounds in immunodeficient mice. Biopsies were analyzed in vitro and at 1, 2, or 3 weeks after grafting. COL7 was undetectable in ESS prepared using all RDEB cells (group 1), and macroscopic blistering was observed by 2 weeks after grafting in ESS containing RDEB cells. COL7 was expressed, in vitro and in vivo, in ESS prepared using combinations of normal + RDEB cells (groups 2 and 3) or all normal cells (group 4). However, transmission electron microscopy revealed structurally normal anchoring fibrils, in vitro and by week 2 in vivo, only in ESS prepared using all normal cells (group 4). The results suggest that although COL7 protein is produced in engineered skin when cells in only one layer express the COL7 gene, formation of structurally normal anchoring fibrils appears to require expression of COL7 in both dermal fibroblasts and epidermal keratinocytes