Modelling the Effect of Process Parameters on the Wet Extrusion and Spheronisation of High-Loaded Nicotinamide Pellets Using a Quality by Design Approach

Open access articleThe aim of the present study was to develop an alternative process to spray granulation in order to prepare high loaded spherical nicotinamide (NAM) pellets by wet extrusion and spheronisation. Therefore, a quality by design approach was implemented to model the effect of the process parameters of the extrusion-spheronisation process on the roundness, roughness and useable yield of the obtained pellets. The obtained results were compared to spray granulated NAM particles regarding their characteristics and their release profile in vitro after the application of an ileocolon targeted shellac coating. The wet extrusion-spheronisation process was able to form highly loaded NAM pellets (80%) with a spherical shape and a high useable yield of about 90%. However, the water content range was rather narrow between 24.7% and 21.3%. The design of experiments (DoE), showed that the spheronisation conditions speed, time and load had a greater impact on the quality attributes of the pellets than the extrusion conditions screw design, screw speed and solid feed rate (hopper speed). The best results were obtained using a low load (15 g) combined with a high rotation speed (900 m/min) and a low time (3–3.5 min). In comparison to spray granulated NAM pellets, the extruded NAM pellets resulted in a higher roughness and a higher useable yield (63% vs. 92%). Finally, the coating and dissolution test showed that the extruded and spheronised pellets are also suitable for a protective coating with an ileocolonic release profile. Due to its lower specific surface area, the required shellac concentration could be reduced while maintaining the release profil

Two-band ferromagnetic Kondo-lattice model for local-moment half-metals

We introduce a two-band Kondo-lattice model to describe ferromagnetic half-metals with local magnetic moments. In a model study, the electronic and magnetic properties are presented by temperature dependent magnetization curves, band-structures, spin polarizations and plasma frequencies. These are obtained from numerically evaluated equations, based on the single-electron Green functions. We show that the mutual influence between the itinerant electrons and the local magnetic moments is responsible for several phase transitions of the half-metals, namely first and second order magnetic phase transitions, as well as half-metal to semiconductor and half-metal to semimetal transitions.Comment: 10 pages, 5 figures, submitted to Journal of Physics: Condensed Matte

Coherent laminar and turbulent motion of toroidal vortex bundles

Motivated by experiments performed in superfluid helium, we study numerically the motion of toroidal bundles of vortex filaments in an inviscid fluid. We find that the evolution of these large-scale vortex structures involves the generalised leapfrogging of the constituent vortex rings. Despite three dimensional perturbations in the form of Kelvin waves and vortex reconnections, toroidal vortex bundles retain their coherence over a relatively large distance (compared to their size), in agreement with experimental observations.Comment: 22 pages, 12 figure

SPH Simulations of Galactic Gaseous Disk with Bar: Distribution and Kinematic Structure of Molecular Clouds toward the Galactic Center

We have performed Smoothed Particle Hydrodynamic (SPH) simulations to study the response of molecular clouds in the Galactic disk to a rotating bar and their subsequent evolution in the Galactic Center (GC) region. The Galactic potential in our models is contributed by three axisymmetric components (massive halo, exponential disk, compact bulge) and a non-axisymmetric bar. These components are assumed to be invariant in time in the frame corotating with the bar. Some noticeable features such as an elliptical outer ring, spiral arms, a gas-depletion region, and a central concentration have been developed due to the influence of the bar. The rotating bar induces non-circular motions of the SPH particles, but hydrodynamic collisions tend to suppress the random components of the velocity. The velocity field of the SPH particles is consistent with the kinematics of molecular clouds observed in HCN (1-0) transition; these clouds are thought to be very dense clouds. However, the l-v diagram of the clouds traced by CO is quite different from that of our SPH simulation, being more similar to that obtained from simulations using collisionless particles. The $l-v$ diagram of a mixture of collisional and collisionless particles gives better reproduction of the kinematic structures of the GC clouds observed in the CO line. The fact that the kinematics of HCN clouds can be reproduced by the SPH particles suggests that the dense clouds in the GC are formed via cloud collisions induced by rotating bar.Comment: 31 pages, 10 pigures, accepted for publication in Ap

VP24-Karyopherin alpha binding affinities differ between Ebolavirus species, nfluencing interferon inhibition and VP24 stability

Zaire ebolavirus (EBOV), Bundibugyo ebolavirus (BDBV), and Reston ebolavirus (RESTV) belong to the same genus but exhibit different virulence properties. VP24 protein, a structural protein present in all family members, blocks interferon (IFN) signaling and likely contributes to virulence. Inhibition of IFN signaling by EBOV VP24 (eVP24) involves its interaction with the NPI-1 subfamily of karyopherin alpha (KPNA) nuclear transporters. Here, we evaluated eVP24, BDBV VP24 (bVP24), and RESTV VP24 (rVP24) interactions with three NPI-1 subfamily KPNAs (KPNA1, KPNA5, and KPNA6). Using purified proteins, we demonstrated that each VP24 binds to each of the three NPI-1 KPNAs. bVP24, however, exhibited approximately 10-fold-lower KPNA binding affinity than either eVP24 or rVP24. Cell-based assays also indicate that bVP24 exhibits decreased KPNA interaction, decreased suppression of IFN induced gene expression, and a decreased half-life in transfected cells compared to eVP24 or rVP24. Amino acid sequence alignments between bVP24 and eVP24 also identified residues within and surrounding the previously defined eVP24-KPNA5 binding interface that decrease eVP24-KPNA affinity or bVP24-KPNA affinity. VP24 mutations that lead to reduced KPNA binding affinity also decrease IFN inhibition and shorten VP24 half-lives. These data identify novel functional differences in VP24-KPNA interaction and reveal a novel impact of the VP24-KPNA interaction on VP24 stability. IMPORTANCE The interaction of Ebola virus (EBOV) VP24 protein with host karyopherin alpha (KPNA) proteins blocks type I interferon (IFN) signaling, which is a central component of the host innate immune response to viral infection. Here, we quantitatively compared the interactions of VP24 proteins from EBOV and two members of the Ebolavirus genus, Bundibugyo virus (BDBV) and Reston virus (RESTV). The data reveal lower binding affinity of the BDBV VP24 (bVP24) for KPNAs and demonstrate that the interaction with KPNA modulates inhibition of IFN signaling and VP24 stability. The effect of KPNA interaction on VP24 stability is a novel functional consequence of this virus-host interaction, and the differences identified between viral species may contribute to differences in pathogenesis

Reconnection and acoustic emission of quantized vortices in superfluid by the numerical analysis of the Gross-Pitaevskii equation

We study numerically the reconnection of quantized vortices and the concurrent acoustic emission by the analysis of the Gross-Pitaevskii equation. Two quantized vortices reconnect following the process similar to classical vortices; they approach, twist themselves locally so that they become anti-parallel at the closest place, reconnect and leave separately.The investigation of the motion of the singular lines where the amplitude of the wave function vanishes in the vortex cores confirms that they follow the above scenario by reconnecting at a point. This reconnection is not contradictory to the Kelvin's circulation theorem, because the potential of the superflow field becomes undefined at the reconnection point. When the locally anti-parallel part of the vortices becomes closer than the healing length, it moves with the velocity comparable to the sound velocity, emits the sound waves and leads to the pair annihilation or reconnection; this phenomena is concerned with the Cherenkov resonance. The vortices are broken up to smaller vortex loops through a series of reconnection, eventually disappearing with the acoustic emission. This may correspond to the final stage of the vortex cascade process proposed by Feynman. The change in energy components, such as the quantum, the compressible and incompressible kinetic energy is analyzed for each dynamics. The propagation of the sound waves not only appears in the profile of the amplitude of the wave function but also affects the field of its phase, transforming the quantum energy due to the vortex cores to the kinetic energy of the phase field.Comment: 11 pages, 16 figures, LaTe