Adverse outcome pathways:opportunities, limitations and open questions

Adverse outcome pathways (AOPs) are a recent toxicological construct that connects, in a formalized, transparent and quality-controlled way, mechanistic information to apical endpoints for regulatory purposes. AOP links a molecular initiating event (MIE) to the adverse outcome (AO) via key events (KE), in a way specified by key event relationships (KER). Although this approach to formalize mechanistic toxicological information only started in 2010, over 200 AOPs have already been established. At this stage, new requirements arise, such as the need for harmonization and re-assessment, for continuous updating, as well as for alerting about pitfalls, misuses and limits of applicability. In this review, the history of the AOP concept and its most prominent strengths are discussed, including the advantages of a formalized approach, the systematic collection of weight of evidence, the linkage of mechanisms to apical end points, the examination of the plausibility of epidemiological data, the identification of critical knowledge gaps and the design of mechanistic test methods. To prepare the ground for a broadened and appropriate use of AOPs, some widespread misconceptions are explained. Moreover, potential weaknesses and shortcomings of the current AOP rule set are addressed (1) to facilitate the discussion on its further evolution and (2) to better define appropriate vs. less suitable application areas. Exemplary toxicological studies are presented to discuss the linearity assumptions of AOP, the management of event modifiers and compensatory mechanisms, and whether a separation of toxicodynamics from toxicokinetics including metabolism is possible in the framework of pathway plasticity. Suggestions on how to compromise between different needs of AOP stakeholders have been added. A clear definition of open questions and limitations is provided to encourage further progress in the field

Training and Labour Force Requirements of Austrian Hotels and Restaurants

During the last economic boom period, more than half of Austria's hotels and restaurants laboured under staff shortages, and only 31 percent felt unaffected by any personnel deficit. The key factors determining recruiting problems were the growth rate and size of an operation and the share of occupational categories as a proportion of overall employment. The strategies pursued by operations to overcome their shortfalls spanned a wide range. Some 19 percent deployed specific recruiting and staff enhancement strategies. This group comprised mostly the large-scale quality operations that expected to achieve above-average growth. Twelve percent had very few specific expectations of their prospective employees and intended to fall back chiefly on foreign and seasonal workers and otherwise mostly failed to develop any proactive strategies to cope with staff bottlenecks. A disproportionate share of this group is made up of winter season operations of the medium quality segment. 22 percent of the operations had not developed any specific requirement profile for their potential staff nor drawn up any detailed strategy to overcome their staff shortage. They recruited mostly from the regional labour market, preferably from the pool of middle-aged workers. The majority of businesses in this group operated solely in the restaurant sector.Tourismus Arbeitsmarkt Umfrage

Removal of Iron, Manganese, Cadmium, and Nickel Ions Using Brewers’ Spent Grain

The human-made pollution of surface and ground waters is becoming an inevitable and persistently urgent problem for humankind and life in general, as these pollutants are also distributed by their natural circulation. For example, from mining activities and metallurgy, toxic heavy metals pollute the environment and present material risk for human health and the environment. Bioadsorbers are an intriguing way to efficiently capture and eliminate these hazards, as they are environmentally friendly, cheap, abundant, and efficient. In this study, we present brewers’ spent grain (BSG) as an efficient adsorber for toxic heavy metal ions, based on the examples of iron, manganese, cadmium, and nickel ions. We uncover the adsorption properties of two different BSGs and investigate thoroughly their chemical and physical properties as well as their efficiency as adsorbers for simulated and real surface waters. As a result, we found that the adsorption behavior of BSG types differs despite almost identical chemistry. Elemental mapping reveals that all components of BSG contribute to the adsorption. Further, both types are not only able to purify water to reach acceptable levels of cleanness, but also yield outstanding adsorption performance for iron ions of 0.2 mmol/g and for manganese, cadmium, and nickel ions of 0.1 mmol/g

Removal of Iron, Manganese, Cadmium, and Nickel Ions Using Brewers’ Spent Grain

The human-made pollution of surface and ground waters is becoming an inevitable and persistently urgent problem for humankind and life in general, as these pollutants are also distributed by their natural circulation. For example, from mining activities and metallurgy, toxic heavy metals pollute the environment and present material risk for human health and the environment. Bioadsorbers are an intriguing way to efficiently capture and eliminate these hazards, as they are environmentally friendly, cheap, abundant, and efficient. In this study, we present brewers’ spent grain (BSG) as an efficient adsorber for toxic heavy metal ions, based on the examples of iron, manganese, cadmium, and nickel ions. We uncover the adsorption properties of two different BSGs and investigate thoroughly their chemical and physical properties as well as their efficiency as adsorbers for simulated and real surface waters. As a result, we found that the adsorption behavior of BSG types differs despite almost identical chemistry. Elemental mapping reveals that all components of BSG contribute to the adsorption. Further, both types are not only able to purify water to reach acceptable levels of cleanness, but also yield outstanding adsorption performance for iron ions of 0.2 mmol/g and for manganese, cadmium, and nickel ions of 0.1 mmol/g