Basolateral carbonic anhydrase IV in the proximal tubule is a glycosylphosphatidylinositol-anchored protein

Carbonic anhydrase (CA) IV facilitates HCO3 reabsorption in the renal proximal tubule by catalyzing the reversible hydration of CO2. CAIV is tethered to cell membranes via a glycosylphosphatidylinositol (GPI) lipid anchor. As there is basolateral as well as apical CAIV staining in proximal tubule, the molecular identity of basolateral CAIV was examined. Biotinylation of confluent monolayers of rat inner medullary collecting duct cells stably transfected with rabbit CAIV showed apical and basolateral CAIV, and in the cell transfectants expressing high levels of CAIV, a transmembrane form was targeted to the basolateral membrane. Basolateral expression of CAIV (∼46kDa) was confirmed in normal kidney tissue by Western blotting of vesicle fractions enriched for basolateral membranes by Percoll density fractionation. We examined the mode of membrane linkage of basolaterally expressed CAIV in the kidney cortex. CAIV detected in basolateral or apical membrane vesicles exhibited similar molecular size by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis following deglycosylation, and was equally sensitive to phosphatidylinositol-specific phospholipase C digestion, indicating that CAIV is expressed on the basolateral membrane as a GPI-anchored protein. Half of the hydratase activity of basolateral vesicles was resistant to SDS denaturation, compatible with being CAIV. Thus, GPI-anchored CAIV resides in the basolateral membrane of proximal tubule epithelia where it may facilitate HCO3 reabsorption via association with kNBC1

Frontline police employees' social construction of client service.

The social construction of frontline employees’ client service plays a major role in organisational success. This study illuminated why frontline personnel are reluctant to accept organisational change which is in line with new policing philosophies. Applying modernist qualitative methodology, and particularly grounded theory within a case study design a ‘process satisfaction model’ was developed with the aim to improve employee satisfaction with internal processes and ultimately service delivery. This model may be used for change in the South African Police Service (SAPS) and other government departments

Low field vortex matter in YBCO: an atomic beam magnetic resonance study

We report measurements of the low field structure of the magnetic vortex lattice in an untwinned YBCO single-crystal platelet. Measurements were carried out using a novel atomic beam magnetic resonance (ABMR) technique. For a 10.7 G field applied parallel to the c-axis of the sample, we find a triangular lattice with orientational order extending across the entire sample. We find the triangular lattice to be weakly distorted by the a-b anisotropy of the material and measure a distortion factor, f = 1.16. Model-experiment comparisons determine a penetration depth, lambda_ab = 140 (+-20) nm. The paper includes the first detailed description of the ABMR technique. We discuss both technical details of the experiment and the modeling used to interpret the measurements.Comment: 44 pages, 13 figures, submitted to Phys. Rev. B Revision includes Postscript wrapped figures + minor typo

Growing Income Inequality Threatens American Education

The first of two articles in consecutive months describes the origins and nature of growing income inequality, and some of its consequences for American children. It documents the increased family income inequality that's occurred over the past 40 years and shows that the increased income disparity has been more than matched by an expanding gap between the amounts of money that low- and high-income parents spend on enrichment activities for their children. It also shows that the growth in income inequality has been accompanied by increasing gaps in academic achievement. The article draws from the first part of the author's recent book, Restoring Opportunity: The Crisis of Inequality and the Challenge for American Education (Harvard Education Press and the Russell Sage Foundation, 2014). The second part to the series, also drawn from Restoring Opportunity, describes ideas based on proven policy approaches that will enable the country to make progress on the enormous task of restoring the educational opportunities that children from low-income families need if they are to lead productive and fulfilling lives. © 2014

A look at the other 90 per cent: Investigating British Sign Language vocabulary knowledge in deaf children from different language learning backgrounds

In this study we present new data on deaf children's receptive and expressive vocabulary knowledge in British Sign Language (BSL) from a sample consisting of children with deaf parents, children with hearing parents, and children with additional needs. Their performance on three BSL vocabulary tasks was compared with (previously reported findings from) a sample of deaf fluent signers. We use these data to assess the effects of some key demographic/ child variables on deaf signing children's vocabulary and discuss findings in the relation to the meaning of 'normative' data and samples for this heterogeneous population. Findings show no effect of the presence of additional disabilities on participants' scores for any of the three tasks. As expected, chronological age is the most significant factor in performance on all vocabulary tasks while the number of deaf relatives only becomes statistically significant for the form recall task. This study contributes to the field of sign language assessment by seeking to identify key variables in heterogeneity and how these variables affect signed vocabulary acquisition with the long-term objective of informing intervention

A Statistical Survey of Hard X-ray Spectral Characteristics of Solar Flares with Two Footpoints

Using RHESSI data, we have analyzed some 172 hard X-ray peaks during 53 solar flares which exhibited a double-footpoint structure. Fitting both footpoints with power-laws, we find that spectral index differences range mostly between 0 to 0.6, and only rarely go beyond. Asymmetries between footpoints were not observed to be significantly dependent on their mean heliographic position, their relative position with respect to each other, nor their orientation with respect to the solar equator. Assuming a symmetric acceleration process, it is also clear that differences in footpoint spectral indices and footpoint flux ratios can seldom be attributed to a difference in column densities between the two legs of a coronal loop. Our results corroborate better the magnetic mirror trap scenario. Moreover, footpoint asymmetries are more marked during times of peak HXR flux than when averaging over the whole HXR burst, suggesting that the magnetic configuration evolves during individual HXR bursts. We observed also a linear correlation between the peak 50-keV flux and the peak GOES 1-8A channel flux, and that HXR burst duration seem correlated with loop length.Comment: 20 pages, 13 figures. Published in Solar Physic

Study of B meson decays to three-body charmless hadronic final states

We report results of a study of charmless B meson decays to three-body KPiPi, KKPi and KKK final states. Measurements of branching fractions for B decays to K+0Pi+Pi-, K+K+K-, K0K+K-, KsKsK+ and KsKsKs final states are presented. The decays B0=>K0K+K-, B+=>KsKsK+ and B0=>KsKsKs are observed for the first time. We also report evidence for B+=>K+K-Pi+ decay. For the three-body final states K0K+K-, KsKsPi+, K+K+Pi- and K-Pi+Pi+ 90% confidence level upper limits are reported. Finally, we discuss the possibility of using the three-body B0=>KsK+K- decay for CP violation studies. The results are obtained with a 78 fb^-1 data sample collected at the Y(4S) resonance by the Belle detector operating at the KEKB asymmetric energy e+e- collider.Comment: 15 pages, 6 figures. To be submitted to PR