The pause-initiation limit restricts transcription activation in human cells.

Eukaryotic gene transcription is often controlled at the level of RNA polymerase II (Pol II) pausing in the promoter-proximal region. Pausing Pol II limits the frequency of transcription initiation ('pause-initiation limit'), predicting that the pause duration must be decreased for transcriptional activation. To test this prediction, we conduct a genome-wide kinetic analysis of the heat shock response in human cells. We show that the pause-initiation limit restricts transcriptional activation at most genes. Gene activation generally requires the activity of the P-TEFb kinase CDK9, which decreases the duration of Pol II pausing and thereby enables an increase in the productive initiation frequency. The transcription of enhancer elements is generally not pause limited and can be activated without CDK9 activity. Our results define the kinetics of Pol II transcriptional regulation in human cells at all gene classes during a natural transcription response

Global positioning system supported pilot's display

The hardware, software, and operation of the Microwave Scanning Beam Landing System (MSBLS) Flight Inspection System Pilot's Display is discussed. The Pilot's Display is used in conjunction with flight inspection tests that certify the Microwave Scanning Beam Landing System used at Space Shuttle landing facilities throughout the world. The Pilot's Display was developed for the pilot of test aircraft to set up and fly a given test flight path determined by the flight inspection test engineers. This display also aids the aircraft pilot when hazy or cloud cover conditions exist that limit the pilot's visibility of the Shuttle runway during the flight inspection. The aircraft position is calculated using the Global Positioning System and displayed in the cockpit on a graphical display

Meteorological satellites

An overview is presented of the meteorological satellite programs that have been evolving from 1958 to the present, and plans for the future meteorological and environmental satellite systems that are scheduled to be placed into service in the early 1980's are reviewed. The development of the TIROS family of weather satellites, including TIROS, ESSA, ITOS/NOAA, and the present TIROS-N (the third generation operational system) is summarized. The contribution of the Nimbus and ATS technology satellites to the development of the operational-orbiting and geostationary satellites is discussed. Included are descriptions of both the TIROS-N and the DMSP payloads currently under development to assure a continued and orderly growth of these systems into the 1980's

Diffusion pathways of hydrogen across the steps of a vicinal Si(001) surface

Hydrogen diffusion across DB steps on Si(001) surfaces is investigated by means of variable-temperature scanning tunneling microscopy and first-principles calculations. Experimentally, the hopping rate for diffusion from the step sites to the Si dimers of the upper terrace was found to be more than one order of magnitude higher than that for diffusion to the lower terrace. This clear preference, opposite to the trend for the respective binding energies, is explained by first-principles calculations that identify a metastable intermediate to be responsible for the unexpected lowering of the energy barrier for upward diffusion

Topological Reverberations in Flat Space-times

We study the role played by multiply-connectedness in the time evolution of the energy E(t) of a radiating system that lies in static flat space-time manifolds M_4 whose t=const spacelike sections M_3 are compact in at least one spatial direction. The radiation reaction equation of the radiating source is derived for the case where M_3 has any non-trivial flat topology, and an exact solution is obtained. We also show that when the spacelike sections are multiply-connected flat 3-manifolds the energy E(t) exhibits a reverberation pattern with discontinuities in the derivative of E(t) and a set of relative minima and maxima, followed by a growth of E(t). It emerges from this result that the compactness in at least one spatial direction of Minkowski space-time is sufficient to induce this type of topological reverberation, making clear that our radiating system is topologically fragile. An explicit solution of the radiation reaction equation for the case where M_3 = R^2 x S^1 is discussed, and graphs which reveal how the energy varies with the time are presented and analyzed.Comment: 16 pages, 4 figures, REVTEX; Added five references and inserted clarifying details. Version to appear in Int. J. Mod. Phys. A (2000

Transcription activation depends on the length of the RNA polymerase II C‐terminal domain

Eukaryotic RNA polymerase II (Pol II) contains a tail‐like, intrinsically disordered carboxy‐terminal domain (CTD) comprised of heptad‐repeats, that functions in coordination of the transcription cycle and in coupling transcription to co‐transcriptional processes. The CTD repeat number varies between species and generally increases with genome size, but the reasons for this are unclear. Here, we show that shortening the CTD in human cells to half of its length does not generally change pre‐mRNA synthesis or processing in cells. However, CTD shortening decreases the duration of promoter‐proximal Pol II pausing, alters transcription of putative enhancer elements, and delays transcription activation after stimulation of the MAP kinase pathway. We suggest that a long CTD is required for efficient enhancer‐dependent recruitment of Pol II to target genes for their rapid activation

Architecture of the RNA polymerase II-Paf1C-TFIIS transcription elongation complex.

The conserved polymerase-associated factor 1 complex (Paf1C) plays multiple roles in chromatin transcription and genomic regulation. Paf1C comprises the five subunits Paf1, Leo1, Ctr9, Cdc73 and Rtf1, and binds to the RNA polymerase II (Pol II) transcription elongation complex (EC). Here we report the reconstitution of Paf1C from Saccharomyces cerevisiae, and a structural analysis of Paf1C bound to a Pol II EC containing the elongation factor TFIIS. Cryo-electron microscopy and crosslinking data reveal that Paf1C is highly mobile and extends over the outer Pol II surface from the Rpb2 to the Rpb3 subunit. The Paf1-Leo1 heterodimer and Cdc73 form opposite ends of Paf1C, whereas Ctr9 bridges between them. Consistent with the structural observations, the initiation factor TFIIF impairs Paf1C binding to Pol II, whereas the elongation factor TFIIS enhances it. We further show that Paf1C is globally required for normal mRNA transcription in yeast. These results provide a three-dimensional framework for further analysis of Paf1C function in transcription through chromatin

Charged pions from Ni on Ni collisions between 1 and 2 AGeV

Charged pions from Ni + Ni reactions at 1.05, 1.45 and 1.93 AGeV are measured with the FOPI detector. The mean $\pi^{\pm}$ multiplicities per mean number of participants increase with beam energy, in accordance with earlier studies of the Ar + KCl and La + La systems. The pion kinetic energy spectra have concave shape and are fitted by the superposition of two Boltzmann distributions with different temperatures. These apparent temperatures depend only weakly on bombarding energy. The pion angular distributions show a forward/backward enhancement at all energies, but not the $\Theta = 90^0$ enhancement which was observed in case of the Au + Au system. These features also determine the rapidity distributions which are therefore in disagreement with the hypothesis of one thermal source. The importance of the Coulomb interaction and of the pion rescattering by spectator matter in producing these phenomena is discussed.Comment: 22 pages, Latex using documentstyle[12pt,a4,epsfig], to appear in Z. Phys.

Genome-wide analysis of RNA polymerase II termination at protein-coding genes.

At the end of protein-coding genes, RNA polymerase (Pol) II undergoes a concerted transition that involves 3′-processing of the pre-mRNA and transcription termination. Here, we present a genome-wide analysis of the 3′-transition in budding yeast. We find that the 3′-transition globally requires the Pol II elongation factor Spt5 and factors involved in the recognition of the polyadenylation (pA) site and in endonucleolytic RNA cleavage. Pol II release from DNA occurs in a narrow termination window downstream of the pA site and requires the “torpedo” exonuclease Rat1 (XRN2 in human). The Rat1-interacting factor Rai1 contributes to RNA degradation downstream of the pA site. Defects in the 3′-transition can result in increased transcription at downstream genes