45 research outputs found
Influence of Coronary Artery Stenosis Severity and Coronary Collateralization on Extent of Chronic Myocardial Scar: Insights from Quantitative Coronary Angiography and Delayed-Enhancement MRI
Three-way differentiation of sister chromatids in endoreduplicated (M3) chromosomes of Bloom syndrome B-lymphoid cell line
The effects of incorporated tritium and bromodeoxyuridine on the frequency of sister chromatid exchanges
Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum)
Catenane’s quantification
17 p.-2 fig. This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at https://doi.org/10.1007/978-1-4939-7459-7_5Two-dimensional agarose gel electrophoresis is the method of choice to identify and quantify all the topological forms DNA molecules can adopt in vivo. Here we describe the materials and protocols needed to analyze catenanes, the natural outcome of DNA replication, in Saccharomyces cerevisiae. We describe the formation of pre-catenanes during replication and how inhibition of topoisomerase 2 leads to the accumulation of intertwined sister duplexes. This knowledge is essential to determine how replication forks blockage or pausing affects the dynamic of DNA topology during replication.This work was supported by grant BFU2014-56835 from the Spanish Ministerio de EconomÃa y Competitividad to JBSPeer reviewe
Detection of replication initiation by a replicon family in DNA of synchronized pea (Pisum sativum) root cells using benzoylated naphthoylated DEAE-cellulose chromatography
Influence of low doses of BrdU and estimation of spontaneous SCE in CHO chromosomes: three-way differential staining and an immunoperoxidase method
Replication of vertebrate mitochondrial DNA entails transient ribonucleotide incorporation throughout the lagging strand
Using two-dimensional agarose gel electrophoresis, we show that mitochondrial DNA (mtDNA) replication of birds and mammals frequently entails ribonucleotide incorporation throughout the lagging strand (RITOLS). Based on a combination of two-dimensional agarose gel electrophoretic analysis and mapping of 5′ ends of DNA, initiation of RITOLS replication occurs in the major non-coding region of vertebrate mtDNA and is effectively unidirectional. In some cases, conversion of nascent RNA strands to DNA starts at defined loci, the most prominent of which maps, in mammalian mtDNA, in the vicinity of the site known as the light-strand origin