Continuity and change in subsistence at Tell Barri, NE Syria

The history of the Fertile Crescent is well documented through archaeology and epigraphy. However, contrary to adjacent regions in the Mediterranean and Middle East, the reconstruction of diet and food ways through isotope analysis is limited for Mesopotamia and, consequently, matters of subsistence change are not well understood. To address this, collagen carbon and nitrogen isotopic ratios of human (N=84) and animal (N=8) samples from Tell Barri, Syria, predominantly ranging from the Early Bronze Age to Roman/Parthian times, were analysed to ascertain diachronic dietary patterns as well as gender- and age-related differences

Animal proxies to characterize the strontium biosphere in the northeastern Nile Delta

Strontium (⁸⁷Sr/⁸⁶Sr) isotope analysis is a potent tool for reconstructing the residential mobility of humans and animals in the past but is reliant on knowledge of strontium isotope variation within the expanded physical environment. This paper aims to contribute to the isoscape in the northeastern Nile Delta with faunal samples from the site of Tell el-Dab‘a (Avaris), believed to be the capital of the so-called Hyksos kings. Mapping the available ⁸⁷Sr/⁸⁶Sr ratios from Egypt and the Sudan highlights major research gaps outside the Nile region. e current corpus of knowledge also shows that the Nile River region yields a homogenous range of isotopic values (median and IQR 0.7076 0.0003). Strontium isotope ratios from human dental enamel, which record childhood residence, will provide evidence of non-locals from outside the Nile area with confidence but these values suggest that identifying movement along the Nile River in the past will be difficult without the use of supplementary evidence (e.g. oxygen stable isotope analysis). We present ⁸⁷Sr/⁸⁶Sr ratios of archaeologically-derived faunal bone samples (n=6) from the site of Tell el-Dab‘a (Avaris) in the northeastern Nile Delta. e ⁸⁷Sr/⁸⁶Sr ratios fit within the expectations of the wider Nile values (mean 0.70769 0.00003) and serve as the first archaeologically-derived values reported for this area of Egypt

Sirt1 activation by resveratrol is substrate sequence-selective

Sirtuins are protein deacetylases used as therapeutic targets. Pharmacological Sirt1 activation has been questioned since the in vitro activator resveratrol failed to stimulate deacetylation of several physiological substrates. We tested the influence of substrate sequence by analyzing resveratrol effects on Sirt1-dependent deacetylation of 6802 physiological acetylation sites using peptide microarrays. Resveratrol stimulated deacetylation of a small set of sites and inhibited deacetylation of another set, whereas most substrates were hardly affected. Solution assays confirmed these substrate categories, and statistical analysis revealed their sequence features. Our results reveal substrate sequence dependence for Sirt1 modulation and suggest substrates contributing to resveratrol effects. ONE SENTENCE SUMMARY: Testing 6802 acetylation sites reveals that resveratrol effects on Sirt1-dependent deacetylation depend on substrate sequence and suggests substrates relevant for in vivo effects

Weapon injuries in the crusader mass graves from a 13th century attack on the port city of Sidon (Lebanon).

Archaeological excavations close to St Louis' castle in Sidon, Lebanon have revealed two mass grave deposits containing partially articulated and disarticulated human skeletal remains. A minimum of 25 male individuals have been recovered, with no females or young children. Radiocarbon dating of the human remains, a crusader coin, and the design of Frankish belt buckles strongly indicate they belong to a single event in the mid-13th century CE. The skeletal remains demonstrate a high prevalence of unhealed sharp force, penetrating force and blunt force trauma consistent with medieval weaponry. Higher numbers of wounds on the back of individuals than the front suggests some were attacked from behind, possibly as they fled. The concentration of blade wounds to the back of the neck of others would be compatible with execution by decapitation following their capture. Taphonomic changes indicate the skeletal remains were left exposed for some weeks prior to being collected together and re-deposited in the defensive ditch by a fortified gateway within the town wall. Charring on some bones provides evidence of burning of the bodies. The findings imply the systematic clearance of partially decomposed corpses following an attack on the city, where adult and teenage males died as a result of weapon related trauma. The skeletons date from the second half of the Crusader period, when Christian-held Sidon came under direct assault from both the Mamluk Sultanate (1253 CE) and the Ilkhanate Mongols (1260 CE). It is likely that those in the mass graves died during one of these assaults

Reconstructing breastfeeding and weaning practices in the Bronze Age Near East using stable nitrogen isotopes.

OBJECTIVES: Breastfeeding and childhood diet have significant impact on morbidity and mortality within a population, and in the ancient Near East, it is possible to compare bioarchaeological reconstruction of breastfeeding and weaning practices with the scant textual evidence. MATERIALS AND METHODS: Nitrogen stable isotopes (δ15 N) are analyzed here for dietary reconstruction in skeletal collections from five Bronze Age (ca. 2,800-1,200 BCE) sites in modern Lebanon and Syria. We employed Bayesian computational modeling on cross-sectional stable isotope data of collagen samples (n = 176) mainly from previous studies to test whether the bioarchaeological evidence aligns with the textual evidence of breastfeeding and weaning practices in the region, as well as compare the estimated weaning times to the global findings using the WARN (weaning age reconstruction with nitrogen isotope analysis) Bayesian model. RESULTS: Though the Near East sites in this study had different ecological settings and economic strategies, we found that weaning was introduced to the five sites at 0.5 ± 0.2 years of age and complete weaning occurred around 2.6 ± 0.3 years of age on using the WARN computational model. These weaning processes are within the time suggested by historical texts, though average estimated weaning age on the Mediterranean coast is later than inland sites. DISCUSSION: Compared globally, these Near Eastern populations initiated the weaning process earlier but completed weaning within the global average. Early initial weaning may have created short spacing between pregnancies and a high impact on demographic growth within these agricultural populations, with some variation in subsistence practices accounting for the inland/coastal discrepancies

Hidden in Bones: Tracking the Hyksos Across the Levant

Though the term Hyksos commonly refers to the rulers of the Egyptian Second Intermediate Period, it is also used to describe the larger population from which these rulers derived. Archaeological, artistic and textual sources suggest a Levantine origin of the ‘Hyksos people’; however, whether this was a single homogenous group or several groups from a wider area has remained uncertain.Non-metric traits, also called epigenetic traits, are normal variations of the human skeleton. The traits, though seemingly random in a single person, often have a genetic component that links biologically closely related people together, enabling an exploration of biological affiliations not only within but also between populations.As a non-invasive method, non-metric trait analysis can be used to study biological closeness when there is no wish or possibility to use DNA analysis. An increasing number of non-metric trait analyses is being conducted on human skeletal remains, creating trait distribution maps for a wider exploration of biological closeness. As part of the Hyksos Enigma Project, non-metric traits from human skeletal remains from Tell el-Dabʿa, the ancient Hyksos capital of Avaris, are recorded. The results help to understand familial relationships within the site but also compare the site and its subgroups to Levantine sites, potentially offering new insight into the Hyksos origin and the wider migration of people in the Levantine area

Vitamin D receptor regulates intestinal proteins involved in cell proliferation, migration and stress response

BACKGROUND: Genome-wide association studies found low plasma levels of 25-hydroxyvitamin D and vitamin D receptor (VDR) polymorphisms associated with a higher prevalence of pathological changes in the intestine such as chronic inflammatory bowel diseases. METHODS: In this study, a proteomic approach was applied to understand the overall physiological importance of vitamin D in the small intestine, beyond its function in calcium and phosphate absorption. RESULTS: In total, 569 protein spots could be detected by two-dimensional-difference in-gel electrophoresis (2D-DIGE), and 82 proteins were considered as differentially regulated in the intestinal mucosa of VDR-deficient mice compared to that of wildtype (WT) mice. Fourteen clearly detectable proteins were identified by MS/MS and further analyzed by western blot and/or real-time RT-PCR. The differentially expressed proteins are functionally involved in cell proliferation, cell adhesion and cell migration, stress response and lipid transport. Mice lacking VDR revealed higher levels of intestinal proteins associated with proliferation and migration such as the 37/67 kDa laminin receptor, collagen type VI (alpha 1 chain), keratin-19, tropomyosin-3, adseverin and higher levels of proteins involved in protein trafficking and stress response than WT mice. In contrast, proteins that are involved in transport of bile and fatty acids were down-regulated in small intestine of mice lacking VDR compared to WT mice. However, plasma and liver concentrations of cholesterol and triglycerides were not different between the two groups of mice. CONCLUSION: Collectively, these data imply VDR as an important factor for controlling cell proliferation, migration and stress response in the small intestine

Whole CMV proteome pattern recognition analysis after HSCT identifies unique epitope targets associated with the CMV status

Cytomegalovirus (CMV) infection represents a vital complication after Hematopoietic Stem Cell Transplantation (HSCT). We screened the entire CMV proteome to visualize the humoral target epitope-focus profile in serum after HSCT. IgG profiling from four patient groups (donor and/or recipient +/- for CMV) was performed at 6, 12 and 24 months after HSCT using microarray slides containing 17174 of 15mer-peptides overlapping by 4 aa covering 214 proteins from CMV. Data were analyzed using maSigPro, PAM and the 'exclusive recognition analysis (ERA)' to identify unique CMV epitope responses for each patient group. The 'exclusive recognition analysis' of serum epitope patterns segregated best 12 months after HSCT for the D+/R+ group (versus D-/R-). Epitopes were derived from UL123 (IE1), UL99 (pp28), UL32 (pp150), this changed at 24 months to 2 strongly recognized peptides provided from UL123 and UL100. Strongly (IgG) recognized CMV targets elicited also robust cytokine production in T-cells from patients after HSCT defined by intracellular cytokine staining (IL-2, TNF, IFN and IL-17). High-content peptide microarrays allow epitope profiling of entire viral proteomes; this approach can be useful to map relevant targets for diagnostics and therapy in patients with well defined clinical endpoints. Peptide microarray analysis visualizes the breadth of B-cell immune reconstitution after HSCT and provides a useful tool to gauge immune reconstitution.The work has been funded by ALF (Arbetslivfonden) to M.M. and P.L. funds from Karolinska Institutet and Vinnova, Sweden to M.M