Microbial interactions with oysters from the Great Bay Estuary: Characterization of endemic Vibrio choleraeand oyster metagenetics

Within the last two decades the number of gastroenteritis seafood-borne outbreaks due to Vibrio species has increased. These infections are usually caused by the consumption of raw or undercooked shellfish. I took two approaches to describe the ecology of Vibrios in the Great Bay Estuary, Durham, New Hampshire. First, I performed a phenotypic and multi-locus sequence analysis of 31 V. cholerae strains isolated from oysters, water and sediment in 2008 and 2009. I concluded that the V. cholerae population is endemic, diverse and highly recombinatory, but none of the isolates contain the virulence markers indicative of clinical strains. Next, I performed a metagenetic microbial census of 39 individual oysters and the overlying water from the Nannie Island and Oyster River oyster beds. I concluded that the microbial populations in the oyster are differential from the overlying water. Furthermore, the microbial populations structure in V. parahaemolyticus contaminated oysters contain unique signatures

Bacterial community profiles and Vibrio parahaemolyticus abundance in individual oysters and their association with estuarine ecology

Oysters naturally harbor the human gastric pathogen Vibrio parahaemolyticus, but the nature of this association is unknown. Because microbial interactions could influence the accumulation of V. parahaemolyticus in oysters, we investigated the composition of the microbiome in water and oysters at two ecologically unique sites in the Great Bay Estuary, New Hampshire using 16s rRNA profiling. We then evaluated correlations between bacteria inhabiting the oyster with V. parahaemolyticus abundance quantified using a most probable number (MPN) analysis. Even though oysters filter-feed, their microbiomes were not a direct snapshot of the bacterial community in overlaying water, suggesting they selectively accumulate some bacterial phyla. The microbiome of individual oysters harvested more centrally in the bay were relatively more similar to each other and had fewer unique phylotypes, but overall more taxonomic and metabolic diversity, than the microbiomes from tributary-harvested oysters that were individually more variable with lower taxonomic and metabolic diversity. Oysters harvested from the same location varied in V. parahaemolyticus abundance, with the highest abundance oysters collected from one location. This study, which to our knowledge is the first of its kind to evaluate associations of V. parahaemolyticus abundance with members of individual oyster microbiomes, implies that sufficient sampling and depth of sequencing may reveal microbiome members that could impact V. parahaemolyticus abundance

Development and Implementation of a Long-Term Freshmen Service Project: The Design and Deployment of an Engineering Outreach Experience for Underserved Populations

Freshmen year programs in engineering have received recent and growing attention as a method to engage first year students in their profession and start them on a path to success. Service learning is a well-known pedagogical method that has been shown to improve retention, especially of underrepresented groups in engineering, and to promote deeper learning through reflection. This paper describes the implementation of a new long-term (full year) service learning project where 240 freshmen mechanical engineering students worked together in small teams to design and deploy an engineering outreach experience for a designated age-group of 4th-11th grade students. This educational experience is designed to expose the freshmen engineering students to a variety of concepts and skills necessary for successful negotiation of their engineering careers. The project encourages the freshmen to challenge their assumptions and conceptions of what an engineer is and does. Other knowledge and skills gained include understanding and using the engineering design process, effectively working on engineering teams, effectively communicating, planning and making decisions, all while solving an open-ended problem. The experience also asks the freshmen to consider diverse perspectives as they design for the targeted populations. The paper describes the project implementation and presents results from student reflections and from a survey. Lessons learned and recommendations for best practices are also presented

Medico-Legal Collaboration Regarding the Sex Offender: Othering and Resistance

We examined medico-legal collaboration regarding dangerous sex offenders where state legislators have adopted statutes that determine the criteria for commitment to and discharge from civil commitment programs. The application of these statutes relies on medical diagnoses of pathologies such as paraphilia, anti-social personality disorder, and pedophilia along with prognoses for cure or recidivism. In our study, we examined court opinions from commitment hearings and observed a trial in federal court on the constitutionality of these commitments. We found that one result of this medico-legal collaboration is the marginalization or othering of sex offenders by essentializing, dividing, shaming, and impeaching them. We also found that this group attempted to resist othering by rhetorical strategies such as providing evidence of change in character, distinction within the othered group, and proof of internal controls over unacceptable impulses. Finally, we discovered that such othering relies heavily on medical expertise, even though some medical practitioners may disagree with, or be hesitant in, their roles in this medico-legal collaboration

The Hidden Cost of University Patents

Universities are encouraged to undertake research through grants from government agencies, foundations, and other organizations. The Bayh-Dole Act reinforces this incentive structure by allowing universities to take ownership of the resultant patents. The rights of ownership include the ability to generate income by licensing patents and bringing patent infringement lawsuits. Undoubtedly, exercising these rights to financially benefit the university is economically rational. But might such actions also impose a cost on the public despite the fact that these very patents arose from public research subsidies? This study examines the relationship between a university’s research expenditures and its likelihood to litigate patent infringement claims. It finds that research expenditures increase litigation frequency, suggesting that universities may use funds earmarked for research and innovation on patent litigation. We argue that patent rights provided by the Bayh-Dole Act may motivate this phenomenon—which encourages universities to seek rents, rather than pursue innovation. Our study adds to the extant literature about firm behavior, describing universities as vertical integrators as well as horizontal coordinators. It further suggests that this coordination inures to a university’s private benefit—but not necessarily the benefit of the public, for which universities are ostensibly organized

Identification of the Microlens in Event MACHO-LMC-20

We report on the identification of the lens responsible for microlensing event MACHO-LMC-20. As part of a \textit{Spitzer}/IRAC program conducting mid-infrared follow-up of the MACHO Large Magellanic Cloud microlensing fields, we discovered a significant flux excess at the position of the source star for this event. These data, in combination with high resolution near-infrared \textit{Magellan}/PANIC data has allowed us to classify the lens as an early M dwarf in the thick disk of the Milky Way, at a distance of $\sim 2$ kpc. This is only the second microlens to have been identified, the first also being a M dwarf star in the disk. Together, these two events are still consistent with the expected frequency of nearby stars in the Milky Way thin and thick disks acting as lenses.Comment: 6 pages, 4 figures, submitted to ApJ Letter

Micropillar compression testing of powders

An experimental design for microcompression on individual powder particles is proposed as a means of testing novel materials without the challenges associated with consolidation to produce bulk specimens. This framework is demonstrated on an amorphous tungsten alloy powder, and yields reproducible measurements of the yield strength (4.5 ± 0.3 GPa) and observations of the deformation mode (in this case, serrated flow by shear localization).United States. Defense Threat Reduction Agency (Grant HDTRA1-11-1-0062)American Society for Engineering Education. National Defense Science and Engineering Graduate Fellowshi

Host-selected mutations converging on a global regulator drive an adaptive leap towards symbiosis in bacteria

Host immune and physical barriers protect against pathogens but also impede the establishment of essential symbiotic partnerships. To reveal mechanisms by which beneficial organisms adapt to circumvent host defenses, we experimentally evolved ecologically distinct bioluminescent Vibrio fischeri by colonization and growth within the light organs of the squid Euprymna scolopes. Serial squid passaging of bacteria produced eight distinct mutations in the binK sensor kinase gene, which conferred an exceptional selective advantage that could be demonstrated through both empirical and theoretical analysis. Squid-adaptive binK alleles promoted colonization and immune evasion that were mediated by cell-associated matrices including symbiotic polysaccharide (Syp) and cellulose. binK variation also altered quorum sensing, raising the threshold for luminescence induction. Preexisting coordinated regulation of symbiosis traits by BinK presented an efficient solution where altered BinK function was the key to unlock multiple colonization barriers. These results identify a genetic basis for microbial adaptability and underscore the importance of hosts as selective agents that shape emergent symbiont populations

Impact of Clinical Factors on 18F-Flotufolastat Detection Rates in Men With Recurrent Prostate Cancer: Exploratory Analysis of the Phase 3 SPOTLIGHT Study.

PURPOSE: 18F-Flotufolastat (18F-rhPSMA-7.3) is a newly approved prostate-specific membrane antigen targeting radiopharmaceutical for diagnostic imaging of prostate cancer (PCa). SPOTLIGHT (National Clinical Trials 04186845) evaluated 18F-flotufolastat in men with suspected PCa recurrence. Here, we present results of predefined exploratory endpoints from SPOTLIGHT to evaluate the impact of clinical factors on 18F-flotufolastat detection rates (DR). METHODS AND MATERIALS: The impact of baseline prostate-specific antigen (PSA), PSA doubling time (PSAdt), and International Society of Urologic Pathology Grade Group (GG) on 18F-flotufolastat DR was evaluated among all SPOTLIGHT patients with an evaluable scan, with DR stratified according to the patients prior treatment (radical prostatectomy ± radiation therapy [RP] or radiation therapy only [RT]). The patients underwent positron emission tomography 50 to 70 minutes after receiving 18F-flotufolastat (296 MBq IV), and scans were read by 3 blinded central readers, with the majority read representing agreement between ≥2 readers. RESULTS: In total, 389 men (median PSA: 1.10 ng/mL) were evaluable. By majority read, 18F-flotufolastat identified distant lesions in 39% and 43% of patients treated with prior RP or RT, respectively. The overall DR broadly increased with increasing PSA (<0.2 ng/mL: 33%; ≥10 ng/mL: 100%). Among patients with PSA <1 ng/mL, 68% had positive scans, and 27% had extrapelvic findings. PSAdt was available for 145/389 (37%) patients. PSAdt did not appear to influence 18F-flotufolastat DR (77%-90% across all PSAdt categories). Among patients with prior RP, DR ranged from 70% to 83% across PSAdt categories, and 100% DR was reported for all post-RT patients. In total, 362/389 (93%) patients had baseline GG data. Overall DRs were uniformly high (75%‒95%) across all GG. When stratified by prior treatment, DRs across all GG were 69% to 89% in patients with prior RP and ≥96% in patients with prior RT. CONCLUSIONS: 18F-Flotufolastat-positron emission tomography enabled the accurate detection of recurrent PCa lesions across a wide range of PSA, PSAdt, and International Society of Urologic Pathology GG, thus supporting its clinical utility for a broad range of patients with recurrent PCa