Insights into antibody catalysis: Structure of an oxygenation catalyst at 1.9-Å resolution

The x-ray crystal structures of the sulfide oxidase antibody 28B4 and of antibody 28B4 complexed with hapten have been solved at 2.2-Å and 1.9-Å resolution, respectively. To our knowledge, these structures are the highest resolution catalytic antibody structures to date and provide insight into the molecular mechanism of this antibody-catalyzed monooxygenation reaction. Specifically, the data suggest that entropic restriction plays a fundamental role in catalysis through the precise alignment of the thioether substrate and oxidant. The antibody active site also stabilizes developing charge on both sulfur and periodate in the transition state via cation-pi and electrostatic interactions, respectively. In addition to demonstrating that the active site of antibody 28B4 does indeed reflect the mechanistic information programmed in the aminophosphonic acid hapten, these high-resolution structures provide a basis for enhancing turnover rates through mutagenesis and improved hapten design

REFLECTIONS ON THE EXPERIENCES AND NEEDS OF GRANDPARENTS CARING FOR THEIR GRANDCHILDREN WITH A SUBSTANCE USE DISORDER

In South Africa grandparents play a significant role in family life. A qualitative study was undertaken to explore and describe the perspectives of grandparents caring for their grandchildren with substance use disorder (SUD). Data were collected by means of semi-structured interviews with grandmothers and analysed following Tesch’s eight steps. Guba’s classic model was employed for data verification. The findings revealed that grandparents experience heightened levels of stress, fear, intimidation, disappointment, shame and financial constraints, which may subsequently compromise their social wellbeing. Based on these findings, recommendations are made in an attempt to provide solutions to the challenges faced by grandparent

The new Iowa interactive Soil Survey

Understanding the basic properties of soils leads to better crop management, soil nutrient management, and soil and water conservation. The Soil Survey includes soil maps and descriptions of each type of soil in each county including interpretations of a soil’s potential for use

The Risk of Cancer from CT Scans and Other Sources of Low-Dose Radiation: A Critical Appraisal of Methodologic Quality

AbstractIntroduction: Concern exists that radiation exposure from computerized tomography (CT) will cause thousands of malignancies. Other experts share the same perspective regarding the risk from additional sources of low-dose ionizing radiation, such as the releases from Three Mile Island (1979; Pennsylvania USA) and Fukushima (2011; Okuma, Fukushima Prefecture, Japan) nuclear power plant disasters. If this premise is false, the fear of cancer leading patients and physicians to avoid CT scans and disaster responders to initiate forcedevacuations is unfounded.Study Objective: This investigation provides a quantitative evaluation of the methodologic quality of studies to determine the evidentiary strength supporting or refuting a causal relationshipbetween low-dose radiation and cancer. It will assess the number of higher qualitystudies that support or question the role of low-dose radiation in oncogenesis.Methods: This investigation is a systematic, methodologic review of articles published from 1975–2017 examining cancer risk from external low-dose x-ray and gamma radiation, defined as less than 200 millisievert (mSv). Following the PRISMA guidelines, the authors performed a search of the PubMed, Cochrane, Scopus, and Web of Science databases. Methodologies of selected articles were scored using the Newcastle Ottawa Scale (NOS) and a tool identifying 11 lower quality indicators. Manuscript methodologies were rankedas higher quality if they scored no lower than seven out of nine on the NOS and contained no more than two lower quality indicators. Investigators then characterized articles as supporting or not supporting a causal relationship between low-dose radiation and cancer.Results: Investigators identified 4,382 articles for initial review. A total of 62 articles met all inclusion/exclusion criteria and were evaluated in this study. Quantitative evaluation of the manuscripts’ methodologic strengths found 25 studies met higher quality criteria while 37 studies met lower quality criteria. Of the 25 studies with higher quality methods, 21 out of 25did not support cancer induction by low-dose radiation (P = .0003).Conclusions: A clear preponderance of articles with higher quality methods found no increased risk of cancer from low-dose radiation. The evidence suggests that exposure to multiple CT scans and other sources of low-dose radiation with a cumulative dose up to 100 mSv (approximately 10 scans), and possibly as high as 200 mSv (approximately 20 scans), does not increase cancer risk

Kinetics and mechanism of substitution reactions of tungsten carbonyl complexes containing bidentate sulfur ligands

Previous investigation of the kinetics and mechanism of the substitution reaction of bidentate substituted transition metal carbonyl complexes with Lewis bases (L') to yield the cis distributed bidentate substitution product, where M=Cr, Mo, or W and S-S = DTO (2,2,7,7-tetra-methyl-3, 6-dithiaoctane) or DTN (2,2,8,8-tetramethyl-3,7-dithianonane) have suggested that this reaction proceeds via a mechanism involving initial reversible dissociation of one end of the bidentate sulfur ligand followed by rapid attack of L' on the resulting five-coordinate intermediate

Considerations for Monitoring School Health and Nutrition Programs.

School health and nutrition (SHN) interventions are among the most ubiquitous public health investments and comprise a key mechanism for reaching populations that are otherwise difficult to reach through the health system. Despite the critical role of monitoring these multisectoral programs to enable data-informed adaptive programming, information to guide program implementers is scant. This manuscript provides an overview of how monitoring indicators can be selected across a SHN program's logical framework, with specific examples across five SHN implementation models. Adaptation of SHN programs in times of school closures, such as those currently being experienced globally due to the emergence of COVID-19, is also addressed. Key aspects of SHN program monitoring are explored, including: (1) why monitor; (2) what to measure; (3) how to measure; and (4) who measures. In situations of school closures, strategies to shift both program activities and corresponding monitoring mechanisms are critical to understanding the rapidly evolving situation and subsequently guiding policy actions to protect vulnerable populations

Attempt to Cross-Foster Gray Wolf, Canis lupus, Pups into Another Wolf Pack

We attempted to cross-foster four 18-19 week-old Gray Wolf (Canis lupus) pups into another Wolf pack 182 km from their natal pack territory. The pup introduction was the result of depredation control on a farm in northwestern Wisconsin. Three pups died within 14 days of release. A fourth pup survived along the edge of the new pack territory over winter, dispersed in the spring, joined or formed a new pack and was captured on a depredation complaint four years later

School Health and Nutrition Monitoring: What Practitioners and Policy Makers Can Learn from China.

“Coverage of School Health Monitoring Systems in China: a Large National Cross-Sectional Survey” by Yan et al. provides an important demonstration of the value of monitoring national school health and nutrition programs. School-based surveys have long been used as cost-efficient monitoring tools, as they serve as a proxy for the broader health and wellbeing for the community in which they live and inform programmatic responses. Despite their importance, there is surprisingly little published on how countries operationally monitor their programs, which presents meaningful challenges for program implementers globally

Development and Pilot of the Caregiver Strategies Inventory

Children with autism spectrum disorder often demonstrate unusual behavioral responses to sensory stimuli (i.e., sensory features). To manage everyday activities, caregivers may implement strategies to address these features during family routines. However, investigation of specific strategies used by caregivers is limited by the lack of empirically developed measures. In this study, we describe the development and pilot results of the Caregiver Strategies Inventory (CSI), a supplement to the Sensory Experiences Questionnaire Version 3.0 (SEQ 3.0; Baranek, 2009) that measures caregivers’ strategies in response to their children’s sensory features. Three conceptually derived and empirically grounded strategy types were tested: cognitive–behavioral, sensory–perceptual, and avoidance. Results indicated that the CSI demonstrated good internal consistency and that strategy use was related to child age and cognition. Moreover, parent feedback after completing the CSI supported its utility and social validity. The CSI may be used alongside the SEQ 3.0 to facilitate a family-centered approach to assessment and intervention planning