1,218 research outputs found

    Using Cone Index Data to Explain Yield Variation Within a Field

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    Rosana G. Moreira, Editor-in-Chief; Texas A&M UniversityThis is a Technical Paper from International Commission of Agricultural Engineering (CIGR, Commission Internationale du Genie Rural) E-Journal Volume 4 (2002): N. Isaac, R. Taylor, S. Staggenborg, M. Schrock, and D. Leikam. Using Cone Index Data to Explain Yield Variation Within a Field. Vol. IV. December 2002

    A mosaic maternal splice donor mutation in the EHMT1 gene leads to aberrant transcripts and to Kleefstra syndrome in the offspring

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    The euchromatic histone-lysine N-methyltransferase 1 (EHMT1) gene was examined in a 3-year-old boy with characteristic clinical features of Kleefstra syndrome. Sequencing of all 27 EHMT1 exons revealed a novel mutation, NM_024757.4:c.2712+1G>A, which affects the splice donor of intron 18. Whereas the index patient is heterozygous for that mutation, his phenotypically normal mother shows tissue-specific mosaicism. Sequencing of EHMT1 RT-PCR products revealed two aberrant transcript variants: in one variant, exon 18 was skipped; in the other, a near-by GT motif was used as splice donor and intronic sequence was inserted between exons 18 and 19. Both transcript variants were found in the patient and his mother. The latter had lower amounts of these transcripts consistent with mosaic status. This is the first description of an EHMT1 point mutation being inherited from a parent with verified mosaicism. The constitutive c.2712+1G>A splice site mutation in EHMT1 is fully pathogenic, and the transcript variants produced do not attenuate the severity of the disease.European Journal of Human Genetics advance online publication, 12 December 2012; doi:10.1038/ejhg.2012.267

    IMPACT EVALUATION FOR THE MANUFACTURED HOUSING ACQUISITION PROGRAM (MAP)

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    This report presents the results of an impact evaluation of the Manufactured Housing Acquisition Program. This evaluation was conducted for Bonneville by Pacific Northwest Laboratory to determine MAP's energy impacts and cost-effectiveness. We conducted a three-tiered analysis of the utility billing data to estimate program electriccty savings. The first (a raw billing data comparison and simple regression analysis) and second (PRISM) tier analyses provided useful findings for the third-tier analysis by which program savings were estimated. The third-tier approach used a conditional demand type regression analysis to analyze monthly energy consumption, taking into account significant factors likely to influence electricrty usage. We used the regression results to estimate energy savings under "normal" weather conditions for each climate zone. We determined cost-effectiveness by calculating levelized costs using a methodology published by Bonneville. We analyzed potential market transformation effects from a conceptual viewpoint. Our results suggested that MAP's market transformation benefits probably reduced the levelized cost to utilities by 40% or more

    Syrian Refugees and the Digital Passage to Europe: Smartphone Infrastructures and Affordances

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    This research examines the role of smartphones in refugees’ journeys. It traces the risks and possibilities afforded by smartphones for facilitating information, communication, and migration flows in the digital passage to Europe. For the Syrian and Iraqi refugee respondents in this France-based qualitative study, smartphones are lifelines, as important as water and food. They afford the planning, navigation, and documentation of journeys, enabling regular contact with family, friends, smugglers, and those who help them. However, refugees are simultaneously exposed to new forms of exploitation and surveillance with smartphones as migrations are financialised by smugglers and criminalized by European policies, and the digital passage is dependent on a contingent range of sociotechnical and material assemblages. Through an infrastructural lens, we capture the dialectical dynamics of opportunity and vulnerability, and the forms of resilience and solidarity, that arise as forced migration and digital connectivity coincide

    Label-free electrochemical monitoring of DNA ligase activity

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    This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential nature in DNA metabolism and their importance to a range of molecular biological methodologies. The electrochemical behavior of DNA at mercury and some amalgam electrodes is strongly influenced by its backbone structure, allowing a perfect discrimination between DNA molecules containing or lacking free ends. This variation in electrochemical behavior has been utilized previously for a sensitive detection of DNA damage involving the sugar-phosphate backbone breakage. Here we show that the same principle can be utilized for monitoring of a reverse process, i.e., the repair of strand breaks by action of the DNA ligases. We demonstrate applications of the electrochemical technique for a distinction between ligatable and unligatable breaks in plasmid DNA using T4 DNA ligase, as well as for studies of the DNA backbone-joining activity in recombinant fragments of E. coli DNA ligase

    Search for Radiative Decays of Cosmic Background Neutrino using Cosmic Infrared Background Energy Spectrum

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    We propose to search for the neutrino radiative decay by fitting a photon energy spectrum of the cosmic infrared background to a sum of the photon energy spectrum from the neutrino radiative decay and a continuum. By comparing the present cosmic infrared background energy spectrum observed by AKARI and Spitzer to the photon energy spectrum expected from neutrino radiative decay with a maximum likelihood method, we obatined a lifetime lower limit of 3.1×10123.1 \times 10^{12} to 3.8×10123.8 \times 10^{12} years at 95% confidence level for the third generation neutrino ν3\nu_3 in the ν3\nu_3 mass range between 50 \mmev and 150 \mmev under the present constraints by the neutrino oscillation measurements. In the left-right symmetric model, the minimum lifetime of ν3\nu_3 is predicted to be 1.5×10171.5 \times 10^{17} years for m3m_3 of 50 \mmev. We studied the feasibility of the observation of the neutrino radiative decay with a lifetime of 1.5×10171.5 \times 10^{17} years, by measuring a continuous energy spectrum of the cosmic infrared background

    Decay constants and mixing parameters in a relativistic model for q\barQ system

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    We extend our recent work, in which the Dirac equation with a ``(asymptotically free) Coulomb + (Lorentz scalar γ0σr\gamma_0\sigma r) linear '' potential is used to obtain the light quark wavefunction for qQˉq\bar Q mesons in the limit mQm_Q\to \infty, to estimate the decay constant fPf_P and the mixing parameter BB of the pseudoscalar mesons. We compare our results for the evolution of fPf_P and BB with the meson mass MPM_P to the non-relativistic formulas for these quantities and show that there is a significant correction in the subasymptotic region. For σ=0.14 GeV2\sigma =0.14{{\rm ~GeV}}^{-2} and \lms =0.240{\rm ~GeV} we obtain: fD=0.371  ,  fDs=0.442  ,  fB=0.301  ,  fBs=0.368 GeVf_D =0.371\; ,\; f_{D_s}=0.442\; ,\; f_B=0.301\; ,\; f_{B_s}=0.368 {\rm ~GeV} and BD=0.88  ,  BDs=0.89  ,  BB=0.95  ,  BBs=0.96  ,  B_D=0.88\; ,\; B_{D_s}=0.89\; ,\; B_B=0.95\; ,\; B_{B_s}=0.96\; ,\; and BK=0.60B_K=0.60.Comment: 13 pages, Latex, 3 figures (included

    Field-induced Ordering in Critical Antiferromagnets

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    Transfer-matrix scaling methods have been used to study critical properties of field-induced phase transitions of two distinct two-dimensional antiferromagnets with discrete-symmetry order parameters: triangular-lattice Ising systems (TIAF) and the square-lattice three-state Potts model (SPAF-3). Our main findings are summarised as follows. For TIAF, we have shown that the critical line leaves the zero-temperature, zero -field fixed point at a finite angle. Our best estimate of the slope at the origin is (dTc/dH)T=H=0=4.74±0.15(dT_c/dH)_{T=H=0} = 4.74 \pm 0.15. For SPAF-3 we provided evidence that the zero-field correlation length diverges as ξ(T0,H=0)exp(a/Tx)\xi(T \to 0, H=0) \simeq \exp (a/T^{x}), with x=1.08±0.13x=1.08 \pm 0.13, through analysis of the critical curve at H0H \neq 0 plus crossover arguments. For SPAF-3 we have also ascertained that the conformal anomaly and decay-of-correlations exponent behave as: (a) H=0: c=1,η=1/3c=1, \eta=1/3; (b) H0:c=1/2,η=1/4H \neq 0: c=1/2, \eta=1/4.Comment: RevTex, 7 pages, 4 eps figures, to be published in Phys. Rev.
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