79 research outputs found
Airway Expression of Smad7, a TGF-β-inducible Inhibitory Molecule of TGF-β Signaling, Decreases after Repeated Airway Antigen Challenges
Transforming growth factor-β (TGF-β) is a profibrogenic cytokine that is involved in airway remodeling largely associated with chronic asthma. Accordingly, regulators of TGF-β activity could also play some role in airway remodeling in asthma. In this study, we investigated expression of Smad 7, a major intracellular inhibitor of TGF-β signaling, in the airways of mouse models of acute and chronic asthma. Sensitized, repeatedly (14 days) ovalbumin (OVA)-inhaled BALB/c mice exhibited evidence of airway remodeling including prominent subepithelial fibrosis associated with airway hyperresponsiveness (AHR) and airway inflammation (chronic asthma model) whereas sensitized, shortly OVA-inhaled BALB/c mice showed only AHR and airway inflammation (acute asthma model). Immunohistochemical analysis showed that Smad 7 immunoreactivity in the airways was increased after the development of acute and chronic asthma models and mainly detected in bronchial epithelial cells. Interestingly, Smad 7 immunoreactivity was significantly less in the airways of chronic asthma model than in those of acute asthma model, which was also confirmed by real-time PCR analysis of Smad 7. In consistent with decreased Smad 7 expression in the airways of chronic asthma model, phosphorylation of Smad 2, a marker of active TGF-β signaling, was increased in bronchial epithelial cells of chronic asthma model when compared with acute asthma model. These results suggest that decreased Smad 7 expression and Smad 2 upregulation in bronchial epithelial cells might result in increased TGF-β activity and contribute to the development of airway remodeling seen in chronic asthma
Gas spectroscopy with integrated frequency monitoring through self-mixing in a terahertz quantum-cascade laser
We demonstrate a gas spectroscopy technique, using self-mixing in a 3.4 terahertz quantum-cascade laser (QCL). All previous QCL spectroscopy techniques have required additional terahertz instrumentation (detectors, mixers, or spectrometers) for system pre-calibration or spectral analysis. By contrast, our system self-calibrates the laser frequency (i.e., with no external instrumentation) to a precision of 630 MHz (0.02%) by analyzing QCL voltage perturbations in response to optical feedback within a 0–800 mm round-trip delay line. We demonstrate methanol spectroscopy by introducing a gas cell into the feedback path and show that a limiting absorption coefficient of ∼1×10⁻⁴ cm⁻¹ is resolvable
F1000Prime recommendation of Adaptation by Loss of Heterozygosity in Saccharomyces cerevisiae Clones Under Divergent Selection.
F1000Prime recommendation of Adaptation by Loss of Heterozygosity in Saccharomyces cerevisiae Clones Under Divergent Selection.
SARS-CoV-2 in vero cells, no quantitation, slices 1-10 of 20 CHYMOTRYPSIN digested
Vero cells infected with SARS-CoV-2 total proteome digested with chymotrypsin, this data was generated on the same samples digested with trypsin and published originally in BioRxiv https://www.biorxiv.org/content/10.1101/2020.03.22.002204v
direct RNA seq data, triplicate of RSV - strain A2 in Calu-3 cells at 48 hours post infection
Raw fastq data from Calue-3 cells infected with RSV strain A
Phosphoprotoemics for SARS COV 2 infected VeroE6 cells
Vero cells infected with SARS CoV 2 at Bristol University March 2020 this phospho preoteome is matched with fastq data and a total proteom
vero cells infected with SARS CoV2 slices 11-20 of 20 slices
Vero cells infected with SARS COV 2 at Bristol University march 2020 slices 11-20 form a 20 slice gel with no quant. matched to a dRNAseq dataset done at the same time
direct RNA seq data, triplicate of RSV - strain A2 in Calu-3 cells at 48 hours post infection
Raw fastq data from Calue-3 cells infected with RSV strain A
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