Airway Expression of Smad7, a TGF-β-inducible Inhibitory Molecule of TGF-β Signaling, Decreases after Repeated Airway Antigen Challenges

Transforming growth factor-β (TGF-β) is a profibrogenic cytokine that is involved in airway remodeling largely associated with chronic asthma. Accordingly, regulators of TGF-β activity could also play some role in airway remodeling in asthma. In this study, we investigated expression of Smad 7, a major intracellular inhibitor of TGF-β signaling, in the airways of mouse models of acute and chronic asthma. Sensitized, repeatedly (14 days) ovalbumin (OVA)-inhaled BALB/c mice exhibited evidence of airway remodeling including prominent subepithelial fibrosis associated with airway hyperresponsiveness (AHR) and airway inflammation (chronic asthma model) whereas sensitized, shortly OVA-inhaled BALB/c mice showed only AHR and airway inflammation (acute asthma model). Immunohistochemical analysis showed that Smad 7 immunoreactivity in the airways was increased after the development of acute and chronic asthma models and mainly detected in bronchial epithelial cells. Interestingly, Smad 7 immunoreactivity was significantly less in the airways of chronic asthma model than in those of acute asthma model, which was also confirmed by real-time PCR analysis of Smad 7. In consistent with decreased Smad 7 expression in the airways of chronic asthma model, phosphorylation of Smad 2, a marker of active TGF-β signaling, was increased in bronchial epithelial cells of chronic asthma model when compared with acute asthma model. These results suggest that decreased Smad 7 expression and Smad 2 upregulation in bronchial epithelial cells might result in increased TGF-β activity and contribute to the development of airway remodeling seen in chronic asthma

Gas spectroscopy with integrated frequency monitoring through self-mixing in a terahertz quantum-cascade laser

We demonstrate a gas spectroscopy technique, using self-mixing in a 3.4 terahertz quantum-cascade laser (QCL). All previous QCL spectroscopy techniques have required additional terahertz instrumentation (detectors, mixers, or spectrometers) for system pre-calibration or spectral analysis. By contrast, our system self-calibrates the laser frequency (i.e., with no external instrumentation) to a precision of 630 MHz (0.02%) by analyzing QCL voltage perturbations in response to optical feedback within a 0–800 mm round-trip delay line. We demonstrate methanol spectroscopy by introducing a gas cell into the feedback path and show that a limiting absorption coefficient of ∼1×10⁻⁴   cm⁻¹ is resolvable

Chadox HIV vaccine data

FASTq files from analysis of candidate HIV vaccine

SARS-CoV-2 in vero cells, no quantitation, slices 1-10 of 20 CHYMOTRYPSIN digested

Vero cells infected with SARS-CoV-2 total proteome digested with chymotrypsin, this data was generated on the same samples digested with trypsin and published originally in BioRxiv https://www.biorxiv.org/content/10.1101/2020.03.22.002204v

direct RNA seq data, triplicate of RSV - strain A2 in Calu-3 cells at 48 hours post infection

Raw fastq data from Calue-3 cells infected with RSV strain A

Phosphoprotoemics for SARS COV 2 infected VeroE6 cells

Vero cells infected with SARS CoV 2 at Bristol University March 2020 this phospho preoteome is matched with fastq data and a total proteom

vero cells infected with SARS CoV2 slices 11-20 of 20 slices

Vero cells infected with SARS COV 2 at Bristol University march 2020 slices 11-20 form a 20 slice gel with no quant. matched to a dRNAseq dataset done at the same time

direct RNA seq data, triplicate of RSV - strain A2 in Calu-3 cells at 48 hours post infection

Raw fastq data from Calue-3 cells infected with RSV strain A