5,821 research outputs found
GM1 Ganglioside Modifies α-Synuclein Toxicity and is Neuroprotective in a Rat α-Synuclein Model of Parkinson\u27s Disease.
While GM1 may interact with α-synuclein in vitro to inhibit aggregation, the ability of GM1 to protect against α-synuclein toxicity in vivo has not been investigated. We used targeted adeno-associated viral vector (AAV) overexpression of human mutant α-synuclein (A53T) in the rat substantia nigra (SN) to produce degeneration of SN dopamine neurons, loss of striatal dopamine levels, and behavioral impairment. Some animals received daily GM1 ganglioside administration for 6 weeks, beginning 24 hours after AAV-A53T administration or delayed start GM1 administration for 5 weeks beginning 3 weeks after AAV-A53T administration. Both types of GM1 administration protected against loss of SN dopamine neurons and striatal dopamine levels, reduced α-synuclein aggregation, and delayed start administration of GM1 reversed early appearing behavioral deficits. These results extend prior positive results in MPTP models, are consistent with the results of a small clinical study of GM1 in PD patients that showed slowing of symptom progression with chronic use, and argue for the continued refinement and development of GM1 as a potential disease modifying therapy for PD
Turbulence transition and the edge of chaos in pipe flow
The linear stability of pipe flow implies that only perturbations of
sufficient strength will trigger the transition to turbulence. In order to
determine this threshold in perturbation amplitude we study the \emph{edge of
chaos} which separates perturbations that decay towards the laminar profile and
perturbations that trigger turbulence. Using the lifetime as an indicator and
methods developed in (Skufca et al, Phys. Rev. Lett. {\bf 96}, 174101 (2006))
we show that superimposed on an overall -scaling predicted and studied
previously there are small, non-monotonic variations reflecting folds in the
edge of chaos. By tracing the motion in the edge we find that it is formed by
the stable manifold of a unique flow field that is dominated by a pair of
downstream vortices, asymmetrically placed towards the wall. The flow field
that generates the edge of chaos shows intrinsic chaotic dynamics.Comment: 4 pages, 5 figure
Visual motherese? Signal-to-noise ratios in toddler-directed television
Younger brains are noisier information processing systems; this means that information for younger individuals has to allow clearer differentiation between those aspects that are required for the processing task in hand (the ‘signal’) and those that are not (the ‘noise’). We compared toddler-directed and adult-directed TV programmes (TotTV/ATV). We examined how low-level visual features (that previous research has suggested influence gaze allocation) relate to semantic information, namely the location of the character speaking in each frame. We show that this relationship differs between TotTV and ATV. First, we conducted Receiver Operator Characteristics analyses and found that feature congestion predicted speaking character location in TotTV but not ATV. Second, we used multiple analytical strategies to show that luminance differentials (flicker) predict face location more strongly in TotTV than ATV. Our results suggest that TotTV designers have intuited techniques for controlling toddler attention using low-level visual cues. The implications of these findings for structuring childhood learning experiences away from a screen are discussed
Susceptibility Provision Enhances Effective De-escalation (SPEED): utilizing rapid phenotypic susceptibility testing in Gram-negative bloodstream infections and its potential clinical impact
Abstract
Objectives
We evaluated the performance and time to result for pathogen identification (ID) and antimicrobial susceptibility testing (AST) of the Accelerate Pheno™ system (AXDX) compared with standard of care (SOC) methods. We also assessed the hypothetical improvement in antibiotic utilization if AXDX had been implemented.
Methods
Clinical samples from patients with monomicrobial Gram-negative bacteraemia were tested and compared between AXDX and the SOC methods of the VERIGENE® and Bruker MALDI Biotyper® systems for ID and the VITEK® 2 system for AST. Additionally, charts were reviewed to calculate theoretical times to antibiotic de-escalation, escalation and active and optimal therapy
Results
ID mean time was 21 h for MALDI-TOF MS, 4.4 h for VERIGENE® and 3.7 h for AXDX. AST mean time was 35 h for VITEK® 2 and 9.0 h for AXDX. For ID, positive percentage agreement was 95.9% and negative percentage agreement was 99.9%. For AST, essential agreement was 94.5% and categorical agreement was 93.5%. If AXDX results had been available to inform patient care, 25% of patients could have been put on active therapy sooner, while 78% of patients who had therapy optimized during hospitalization could have had therapy optimized sooner. Additionally, AXDX could have reduced time to de-escalation (16 versus 31 h) and escalation (19 versus 31 h) compared with SOC.
Conclusions
By providing fast and reliable ID and AST results, AXDX has the potential to improve antimicrobial utilization and enhance antimicrobial stewardship
Magnetic White Dwarfs from the SDSS II. The Second and Third Data Releases
Fifty-two magnetic white dwarfs have been identified in spectroscopic
observations from the Sloan Digital Sky Survey (SDSS) obtained between mid-2002
and the end of 2004, including Data Releases 2 and 3. Though not as numerous
nor as diverse as the discoveries from the first Data Release, the collection
exhibits polar field strengths ranging from 1.5MG to ~1000MG, and includes two
new unusual atomic DQA examples, a molecular DQ, and five stars that show
hydrogen in fields above 500MG. The highest-field example, SDSSJ2346+3853, may
be the most strongly magnetic white dwarf yet discovered. Analysis of the
photometric data indicates that the magnetic sample spans the same temperature
range as for nonmagnetic white dwarfs from the SDSS, and support is found for
previous claims that magnetic white dwarfs tend to have larger masses than
their nonmagnetic counterparts. A glaring exception to this trend is the
apparently low-gravity object SDSSJ0933+1022, which may have a history
involving a close binary companion.Comment: 20 pages, 4 figures Accepted for publication in the Astronomical
Journa
An Empirical Calibration of the Completeness of the SDSS Quasar Survey
Spectra of nearly 20000 point-like objects to a Galactic reddening corrected
magnitude of i=19.1 have been obtained to test the completeness of the SDSS
quasar survey. The spatially-unresolved objects were selected from all regions
of color space, sparsely sampled from within a 278 sq. deg. area of sky covered
by this study. Only ten quasars were identified that were not targeted as
candidates by the SDSS quasar survey (including both color and radio source
selection). The inferred density of unresolved quasars on the sky that are
missed by the SDSS algorithm is 0.44 per sq. deg, compared to 8.28 per sq. deg.
for the selected quasar density, giving a completeness of 94.9(+2.6,-3.8) to
the limiting magnitude. Omitting radio selection reduces the color-only
selection completeness by about 1%. Of the ten newly identified quasars, three
have detected broad absorption line systems, six are significantly redder than
other quasars at the same redshift, and four have redshifts between 2.7 and 3.0
(the redshift range where the SDSS colors of quasars intersect the stellar
locus). The fraction of quasars missed due to image defects and blends is
approximately 4%, but this number varies by a few percent with magnitude.
Quasars with extended images comprise about 6% of the SDSS sample, and the
completeness of the selection algorithm for extended quasars is approximately
81%, based on the SDSS galaxy survey. The combined end-to-end completeness for
the SDSS quasar survey is approximately 89%. The total corrected density of
quasars on the sky to i=19.1 is estimated to be 10.2 per sq. deg.Comment: 37 pages, 10 figures, accepted for publication in A
Additional Ultracool White Dwarfs Found in the Sloan Digital Sky Survey
We identify seven new ultracool white dwarfs discovered in the Sloan Digital
Sky Survey (SDSS). The SDSS photometry, spectra, and proper motions are
presented, and additional BVRI data are given for these and other previously
discovered ultracool white dwarfs. The observed colors span a remarkably wide
range, qualitatively similar to colors predicted by models for very cool white
dwarfs. One of the new stars (SDSS J1251+44) exhibits strong collision-induced
absorption (CIA) in its spectra, while the spectra and colors of the other six
are consistent with mild CIA. Another of the new discoveries (SDSS J2239+00A)
is part of a binary system -- its companion is also a cool white dwarf, and
other data indicate that the companion exhibits an infrared flux deficiency,
making this the first binary system composed of two CIA white dwarfs. A third
discovery (SDSS J0310-00) has weak Balmer emission lines. The proper motions of
all seven stars are consistent with membership in the disk or thick disk.Comment: Accepted for Astrophysical Journal. 16 pages (includes 3 figures
Four problems with global carbon markets: a critical review
This article offers a critique of global carbon markets and trading, with a special focus on the Clean Development Mechanism of the Kyoto Protocol. It explores
problems with the use of tradable permits to address climate change revolving around four areas: homogeneity, justice, gaming, and information. Homogeneity problems arise from the non-linear nature of climate change and sensitivity of emissions, which complicate attempts to calculate carbon offsets. Justice problems involve issues of dependency and the concentration of wealth among the rich, meaning carbon trading often counteracts attempts to reduce poverty. Gaming problems include pressures to promote high-volume, least-cost projects and the
consequences of emissions leakage. Information problems encompass transaction costs related to carbon trading and market participation and the comparatively weak institutional capacity of project evaluators
New Constraints on Cosmic Reionization from the 2012 Hubble Ultra Deep Field Campaign
Understanding cosmic reionization requires the identification and
characterization of early sources of hydrogen-ionizing photons. The 2012 Hubble
Ultra Deep Field (UDF12) campaign has acquired the deepest infrared images with
the Wide Field Camera 3 aboard Hubble Space Telescope and, for the first time,
systematically explored the galaxy population deep into the era when cosmic
microwave background (CMB) data indicates reionization was underway. The UDF12
campaign thus provides the best constraints to date on the abundance,
luminosity distribution, and spectral properties of early star-forming
galaxies. We synthesize the new UDF12 results with the most recent constraints
from CMB observations to infer redshift-dependent ultraviolet (UV) luminosity
densities, reionization histories, and electron scattering optical depth
evolution consistent with the available data. Under reasonable assumptions
about the escape fraction of hydrogen ionizing photons and the intergalactic
medium clumping factor, we find that to fully reionize the universe by redshift
z~6 the population of star-forming galaxies at redshifts z~7-9 likely must
extend in luminosity below the UDF12 limits to absolute UV magnitudes of
M_UV\sim -13 or fainter. Moreover, low levels of star formation extending to
redshifts z~15-25, as suggested by the normal UV colors of z\simeq7-8 galaxies
and the smooth decline in abundance with redshift observed by UDF12 to
z\simeq10, are additionally likely required to reproduce the optical depth to
electron scattering inferred from CMB observations.Comment: Version accepted by ApJ (originally submitted Jan 5, 2013). The UDF12
website can be found at http://udf12.arizona.ed
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