1,133 research outputs found
Identification and refinement of two regions on chromosomal arm 15q involved in breast cancer progression
Infrared Spectroscopy of Molecular Supernova Remnants
We present Infrared Space Observatory spectroscopy of sites in the supernova
remnants W28, W44, and 3C391, where blast waves are impacting molecular clouds.
Atomic fine-structure lines were detected from C, N, O, Si, P, and Fe. The S(3)
and S(9) lines of H2 were detected for all three remnants. The observations
require both shocks into gas with moderate (~ 100 /cm3) and high (~10,000 /cm3)
pre-shock densities, with the moderate density shocks producing the ionic lines
and the high density shock producing the molecular lines. No single shock model
can account for all of the observed lines, even at the order of magnitude
level. We find that the principal coolants of radiative supernova shocks in
moderate-density gas are the far-infrared continuum from dust grains surviving
the shock, followed by collisionally-excited [O I] 63.2 and [Si II] 34.8 micron
lines. The principal coolant of the high-density shocks is
collisionally-excited H2 rotational and ro-vibrational line emission. We
systematically examine the ground-state fine structure of all cosmically
abundant elements, to explain the presence or lack of all atomic fine lines in
our spectra in terms of the atomic structure, interstellar abundances, and a
moderate-density, partially-ionized plasma. The [P II] line at 60.6 microns is
the first known astronomical detection. There is one bright unidentified line
in our spectra, at 74.26 microns. The presence of bright [Si II] and [Fe II]
lines requires partial destruction of the dust. The required gas-phase
abundance of Fe suggests 15-30% of the Fe-bearing grains were destroyed. The
infrared continuum brightness requires ~1 Msun of dust survives the shock,
suggesting about 1/3 of the dust mass was destroyed, in agreement with the
depletion estimate and with theoretical models for dust destruction.Comment: 40 pages; 10 figures; accepted by ApJ July 11, 200
Screening of antioxidant properties of the apple juice using the front-face synchronous fluorescence and chemometrics
Fluorescence spectroscopy is gaining increasing attention in food analysis due to its higher sensitivity and selectivity as compared to other spectroscopic techniques. Synchronous scanning fluorescence technique is particularly useful in studies of multi-fluorophoric food samples, providing a further improvement of selectivity by reduction in the spectral overlapping and suppressing light-scattering interferences. Presently, we study the feasibility of the prediction of the total phenolics, flavonoids, and antioxidant capacity using front-face synchronous fluorescence spectra of apple juices. Commercial apple juices from different product ranges were studied. Principal component analysis (PCA) applied to the unfolded synchronous fluorescence spectra was used to compare the fluorescence of the entire sample set. The regression analysis was performed using partial least squares (PLS1 and PLS2) methods on the unfolded total synchronous and on the single-offset synchronous fluorescence spectra. The best calibration models for all of the studied parameters were obtained using the PLS1 method for the single-offset synchronous spectra. The models for the prediction of the total flavonoid content had the best performance; the optimal model was obtained for the analysis of the synchronous fluorescence spectra at Delta lambda = 110 nm (R (2) = 0.870, residual predictive deviation (RPD) = 2.7). The optimal calibration models for the prediction of the total phenolic content (Delta lambda = 80 nm, R (2) = 0.766, RPD = 2.0) and the total antioxidant capacity (Delta lambda = 70 nm, R (2) = 0.787, RPD = 2.1) had only an approximate predictive ability. These results demonstrate that synchronous fluorescence could be a useful tool in fast semi-quantitative screening for the antioxidant properties of the apple juices.info:eu-repo/semantics/publishedVersio
Strong evidence that the common variant S384F in BRCA2 has no pathogenic relevance in hereditary breast cancer
INTRODUCTION: Unclassified variants (UVs) of unknown clinical significance are frequently detected in the BRCA2 gene. In this study, we have investigated the potential pathogenic relevance of the recurrent UV S384F (BRCA2, exon 10). METHODS: For co-segregation, four women from a large kindred (BN326) suffering from breast cancer were analysed. Moreover, paraffin-embedded tumours from two patients were analysed for loss of heterozygosity. Co-occurrence of the variant with a deleterious mutation was further determined in a large data set of 43,029 index cases. Nature and position of the UV and conservation among species were evaluated. RESULTS: We identified the unclassified variant S384F in three of the four breast cancer patients (the three were diagnosed at 41, 43 and 57 years of age). One woman with bilateral breast cancer (diagnosed at ages 32 and 50) did not carry the variant. Both tumours were heterozygous for the S384F variant, so loss of the wild-type allele could be excluded. Ser384 is not located in a region of functional importance and cross-species sequence comparison revealed incomplete conservation in the human, dog, rodent and chicken BRCA2 homologues. Overall, the variant was detected in 116 patients, five of which co-occurred with different deleterious mutations. The combined likelihood ratio of co-occurrence, co-segregation and loss of heterozygosity revealed a value of 1.4 × 10(-8 )in favour of neutrality of the variant. CONCLUSION: Our data provide conclusive evidence that the S384F variant is not a disease causing mutation
The Heavy Element Enrichment of Lyman alpha Clouds in the Virgo Supercluster
Using high S/N STIS echelle spectra (FWHM=7 km/s) of 3C 273, we constrain the
metallicities of two Lya clouds in the vicinity of the Virgo cluster. We detect
C II, Si II, and Si III absorption lines in the Lya absorber at z = 0.00530.
Previous observations with FUSE have revealed Ly beta - Ly theta lines at this
redshift, thereby accurately constraining N(H I). We model the ionization of
the gas and derive [C/H] = -1.2^{+0.3}_{-0.2}, [Si/C] = 0.2+/-0.1, and log
n_{H} = -2.8+/-0.3. The model implies a small absorber thickness, ~70 pc, and
thermal pressure p/k ~ 40 cm^{-3} K. It is most likely that the absorber is
pressure confined by an external medium because gravitational confinement would
require a very high ratio of dark matter to baryonic matter. Based on Milky Way
sight lines in which carbon and silicon abundances have been reliably measured
in the same interstellar cloud (including new measurements presented herein),
we argue that the overabundance of Si relative to C is not due to dust
depletion. Instead, this probably indicates that the gas has been predominately
enriched by Type II supernovae. Such enrichment is most plausibly provided by
an unbound galactic wind, given the absence of galaxies within a projected
distance of 100 kpc and the presence of galaxies capable of driving a wind at
larger distances. We also constrain the metallicity and physical conditions of
the Virgo absorber at z = 0.00337 based on detections of O VI and H I and an
upper limit on C IV. If this absorber is collisionally ionized, the O VI/C IV
limit requires T > 10^{5.3} K. For either collisional ionization or
photoionization, we find that [O/H] > -2.0 at z = 0.00337.Comment: Final Ap.J. versio
Intermediate- and High-Velocity Ionized Gas toward zeta Orionis
We combine UV spectra obtained with the HST/GHRS echelle, IMAPS, and
Copernicus to study the abundances and physical conditions in the predominantly
ionized gas seen at high (-105 to -65 km/s) and intermediate velocities (-60 to
-10 km/s) toward zeta Ori. We have high resolution (FWHM ~ 3.3-4.5 km/s) and/or
high S/N spectra for at least two significant ions of C, N, Al, Si, S, and Fe
-- enabling accurate estimates for both the total N(H II) and the elemental
depletions. C, N, and S have essentially solar relative abundances; Al, Si, and
Fe appear to be depleted by about 0.8, 0.3-0.4, and 0.95 dex, respectively.
While various ion ratios would be consistent with collisional ionization
equilibrium (CIE) for T ~ 25,000-80,000 K, the widths of individual
high-velocity absorption components indicate that T ~ 9000 K -- so the gas is
not in CIE. Analysis of the C II fine-structure excitation equilibrium yields
estimated densities (n_e ~ n_H ~ 0.1-0.2 cm^{-3}), thermal pressures (2 n_H T ~
2000-4000 cm^{-3}K), and thicknesses (0.5-2.7 pc) for the individual clouds. We
compare the abundances and physical properties derived for these clouds with
those found for gas at similar velocities toward 23 Ori and tau CMa, and also
with several models for shocked gas. While the shock models can reproduce some
features of the observed line profiles and some of the observed ion ratios,
there are also significant differences. The measured depletions suggest that
\~10% of the Al, Si, and Fe originally locked in dust in the pre-shock medium
may have been returned to the gas phase, consistent with predictions for the
destruction of silicate dust in a 100 km/s shock. The near-solar gas phase
abundance of carbon, however, seems inconsistent with the predicted longer time
scales for the destruction of graphite grains.Comment: 50 pages, 9 figures; aastex; accepted by Ap
Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects
The purpose of this study was to assess the technical aspects related to polar body (PB) biopsy, which might have an influence on the results of the microarray comparative genomic hybridization analysis. Furthermore, a comparison was made between two biopsy methods (mechanical and laser). Biopsy of the first and second PB (PB1 and PB2) was performed by mechanical- or laser-assisted biopsy in two different IVF centres. PBs were separately amplified by whole genome amplification. The method of biopsy, mechanical or laser had no influence on the proportion of successfully biopsied oocytes. Especially, for the PB2, the timing of biopsy after ICSI was directly correlated to amplification efficiency. Special care has to be taken with respect to the timing of biopsy of the PB2. Mechanical- and laser-assisted biopsy give the same performance in terms of diagnostic efficienc
Associations of common breast cancer susceptibility alleles with risk of breast cancer subtypes in BRCA1 and BRCA2 mutation carriers
Peer reviewedPublisher PD
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