Genetic analysis of rab7 mutants in zebrafish

Vascular network formation requires the fusion of newly formed blood vessels and the emergence of a patent lumen between the newly established connections so that blood flow can start. Lumen formation has been shown to depend on the late endosomal/lysosomal pathway in various organs of animal tubular systems. Here, we identified a late endosomal/lysosomal vesicular fraction (Rab7/Lamp2) in early zebrafish angiogenic sprouts, which appears to contribute to apical membrane growth during lumen formation. To study the effect of the late endocytic pathway on vascular development, we generated mutant alleles for all three rab7 genes in zebrafish ( rab7a, rab7ba, rab7bb ). All rab7 genes are expressed in wild-type zebrafish and we did not detect any compensatory effects by the other rab7 isoforms in single knockout mutants, which were all viable. Only the triple mutant was lethal suggesting some functional redundancy. However, the different rab7 isoforms fulfil also at least partially independent functions because eggs laid from mothers lacking two rab7 ( rab7a and/or rab7bb ). showed reduced survival and contained enlarged yolk granules, suggesting maternal contribution of these two rab7 . Finally, we observed minor effects on lumen formation in embryos which still express one copy of rab7 . Our results support the notion that the late endocytic/lysosomal compartment contributes to lumen expansion

Measurement of CP violation parameters and polarisation fractions in Bs0→J/ψK‾∗0 {\mathrm{B}}_{\mathrm{s}}^0\to \mathrm{J}/\psi {\overline{\mathrm{K}}}^{\ast 0} decays

The first measurement of ${C\!P}$ asymmetries in the decay ${B_s^0\to J/\psi \overline{K}^{*}(892)^{0}}$ and an updated measurement of its branching fraction and polarisation fractions are presented. The results are obtained using data corresponding to an integrated luminosity of $3.0\,fb^{-1}$ of proton-proton collisions recorded with the LHCb detector at centre-of-mass energies of $7$ and $8\,\mathrm{TeV}$. Together with constraints from ${B^0\to J/\psi \rho^0}$, the results are used to constrain additional contributions due to penguin diagrams in the ${C\!P}$-violating phase ${{\phi}_{s}}$, measured through ${B_s^0}$ decays to charmonium.The first measurement of CP asymmetries in the decay ${B}_s^0\to J/\psi {\overline{\mathrm{K}}}^{\ast }{(892)}^0$ and an updated measurement of its branching fraction and polarisation fractions are presented. The results are obtained using data corresponding to an integrated luminosity of 3.0 fb^{−}^{1} of proton-proton collisions recorded with the LHCb detector at centre-of-mass energies of 7 and 8 TeV. Together with constraints from B$^{0}$ → J/ψ ρ$^{0}$, the results are used to constrain additional contributions due to penguin diagrams in the CP -violating phase ϕ$_{s}$ , measured through B$_{s}^{0}$ decays to charmonium.The first measurement of ${C\!P}$ asymmetries in the decay ${B_s^0\to J/\psi \overline{K}^{*}(892)^{0}}$ and an updated measurement of its branching fraction and polarisation fractions are presented. The results are obtained using data corresponding to an integrated luminosity of $3.0\,fb^{-1}$ of proton-proton collisions recorded with the LHCb detector at centre-of-mass energies of $7$ and $8\,\mathrm{TeV}$. Together with constraints from ${B^0\to J/\psi \rho^0}$, the results are used to constrain additional contributions due to penguin diagrams in the ${C\!P}$-violating phase ${{\phi}_{s}}$, measured through ${B_s^0}$ decays to charmonium

Der Kampf der revolutionaeren deutschen Arbeiterjugend auf dem Gebiet der Berufsausbildung 1918-1923

Archiv des IfZJ, Berlin: EA 53 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Erfahrungen und Probleme der staatlichen Leitung Planung und Organisation der Wissenschafts- und Forschungsarbeit auf dem Gebiet der Berufsbildung in der DDR

HUB(11) - 75 HB 4466 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

A Novel Alloy Development Approach: Biomedical Equiatomic Ta-Nb-Ti Alloy

In the present manuscript, we report on the properties of an equiatomic Ta-Nb-Ti alloy as the basis for a novel, biomedical, multi-component alloy development. The alloy was produced using an arc melting furnace under Ar atmosphere, metallographically prepared, and investigated respectively. Furthermore, the alloy produced, as well as samples of elemental Ta, Nb, alloy Co-28Cr-6Mo, and alloy Ti-6Al-4V, were prepared with defined 1200 grit SiC grinding paper. The topography of the surfaces was evaluated using confocal microscopy and contact angle measurements subsequently. Afterwards, the biocompatibility of the novel alloy Ta-Nb-Ti was evaluated by means of cell (osteoblast) attachment as well as monocyte inflammatory response analysis. First results indicate competitive osteoblast attachment, as well as comparable expressions of fibrosis markers in comparison to conventionally used biomedical materials. In addition, the Ta-Nb-Ti alloy showed a markedly reduced inflammatory capacity, indicating a high potential for use as a prospective biomedical material

Bildungs- und Erziehungsaufgaben bei der Entwicklung marxistisch-leninistischer Grundpositionen zur Stellung der Frau in der entwickelten sozialistischen Gesellschaft bei Werktaetigen in sozialistischen Industriebetrieben der DDR

HUB(11) - 72 HB 2752, T. 1, 2 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Vivant professores : BaRock’N’Roll za četvrt stoljeća (Laura Vadjon (barokna violina) i Pavao Mašić (čembalo, orgulje), 10.11.2020.)

Koncert iz ciklusa Vivant professores održan na Muzičkoj akademiji u Koncertnoj dvorani "Blagoje Bersa" 10.11.2020. Izvođači: Laura Vadjon (barokna violina), Pavao Mašić (čembalo, orgulje). Program: 1. Johann Heinrich Schmelzer: Sonata quarta; 2. Dario Castello: Sonata prima; 3. Marco Uccellini: Sonata detta la Laura rilucente; 4. G. F. Handel: Sonata u D-duru, op. 1, br. 13, HWV 371 (Affetuoso – Allegro – Larghetto – Allegro); 5. G. F. Handel: Suita u F-duru za čembalo solo, HWV 427 (Adagio – Allegro – Adagio – Allegro); 6. G. F. Handel: Sonata u d-molu, HWV 359a (Grave – Allegro – Adagio – Allegro); 7. Dario Castello: Sonata seconda; 8. Arcangelo Corelli: Sonata op.5, br. 12 "La Folia"

Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance

Endothelial cells (ECs) line the inside of blood vessels and respond to mechanical cues generated by blood flow. Mechanical stimuli regulate the localization of YAP by reorganizing the actin cytoskeleton. Here we demonstrate blood-flow-mediated regulation of endothelial YAP in vivo. We indirectly monitored transcriptional activity of Yap1 (zebrafish YAP) and its spatiotemporal localization in living zebrafish and found that Yap1 entered the nucleus and promoted transcription in response to blood flow. In cultured human ECs, laminar shear stress induced nuclear import of YAP and its transcriptional activity in a manner independent of Hippo signaling. We uncovered a molecular mechanism by which flow induced the nuclear translocation of YAP through the regulation of filamentous actin and angiomotin. Yap1 mutant zebrafish showed a defect in vascular stability, indicating an essential role for Yap1 in blood vessels. Our data imply that endothelial Yap1 functions in response to flow to maintain blood vessels