Stimulating authentic community involvement in biotechnology policy in Australia

This paper analyzes community involvement in biotechnology policy in Australia. Specifically, we examine the Gene Technology Act 2000 that governs gene technology in Australia and the roles of the Office of the Gene Technology Regulator and the Gene Technology Community Consultative Committee. We contend that the institutions that underpin biotechnology policy serve to hamper community involvement and reinforce a cognitive deficit model of community involvement. Finally, we propose how the situation could be turned around so that interested citizens can participate in authentic community involvement in biotechnology policy in Australia

A new web-based genomics resource for bioinformatics analysis of Rhipicephalus (Boophilus) microplus: CattleTickBase

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Prompting transdisciplinary research: Promising futures for using the performance metaphor in research

Transdisciplinary research is increasingly recognised as important for investigating and addressing 'wicked' problems such as climate change, food insecurity and poverty, but is far from commonplace. There are structural impediments to transdisciplinarity such as university structures, publication requirements and funding preferences that perpetuate disciplinary differences and researchers often lack transdisciplinary experience and expertise. In this paper we present a heuristic that aims to encourage researchers to think about their current research as performance and then imagine different performances, with the view to encouraging reflection and creativity about the transdisciplinary potential and dilemmas. The heuristic is inspired by the metaphor of performance that Erving Goffman uses to understand everyday, face-to-face interactions. The heuristic includes scaffolding for imagining research as performance through a transdisciplinary lens, a suggested process for using the tool, and examples based on the every day research projects. The paper describes the application of the heuristic in a graduate masterclass, reflecting on whether it does indeed 'prompt' transdisciplinary research. Limitations and lessons learned for further refinement of the heuristic are also included. The authors conclude that the heuristic has a range of uses including for self-reflection, and as a practical learning tool that can also be used at the start of integrative research projects

Medium Alt-Az Telescope Control System Standardization. A case Study: The TT1 Control System

The TT1 (Toppo Telescope #1) is a 1.54 m Alt-Az telescope designed and built by the Technology Working Group (TWG) of the Astronomical Observatory of Capodimonte (OAC), Naples Italy. The standardization process is of course one of the fundamental requirements for telescope control system design and development, well considered in the TT1 design. In this paper the approach used to identify a control system applicable to medium-size Alt-Az telescope is presented

Analysis of the Effect of a Sunscreen Agent on the Suppression of Natural Killer Cell Activity Induced in Human Subjects by Radiation from Solarium Lamps

Previous studies in rodents have shown that ultraviolet radiation (UVR) may have direct effects on the immune system in the skin and at higher doses may induce systemic suppression of immune responses. We have previously shown that UVR from sun or solarium beds may induce systemic effects in human subjects. The purpose of the present study was to examine whether these systemic effects in human subjects could be prevented by use of commercially available sunscreen agents. Groups of 12 normal subjects were exposed to radiation from solarium lamps after application of a sunscreen agent or the base used in its preparation. Twelve half-hourly exposures induced a depression of natural killer (NK) cell activity against a melanoma and the K562 target cell which was not prevented by use of the sunscreen agent. Changes in functional activity were accompanied by a reduction in NK cell numbers assessed by Leu-11 monoclonal antibodies against the labile Fc receptor. Application of the sunscreen agent also did not protect against effects of solarium exposure on recall antigen skin tests and immunoglobulin production in vitro in pokeweed mitogen-stimulated cultures of B and T cells. These results suggest that further evaluation of the wavelength spectrum of UVR and the effectiveness of sunscreen agents in prevention of UVR-induced effects on the immune system is needed

The interaction of Epstein-Barr virus encoded transcription factor EBNA2 with multiple sclerosis risk loci is dependent on the risk genotype

Background: Epstein-Barr virus (EBV) infection may be necessary for the development of Multiple sclerosis (MS). Earlier we had identified six MS risk loci that are co-located with binding sites for the EBV transcription factor Epstein-Barr Nuclear Antigen 2 (EBNA2) in EBV-infected B cells (lymphoblastoid cell lines – LCLs). Methods: We used an allele-specific chromatin immunoprecipitation PCR assay to assess EBNA2 allelic preference. We treated LCLs with a peptide inhibitor of EBNA2 (EBNA2-TAT), reasoning that inhibiting EBNA2 function would alter gene expression at these loci if it was mediated by EBNA2. Findings: We found that EBNA2 binding was dependent on the risk allele for five of these six MS risk loci (p < 0·05). Treatment with EBNA2-TAT significantly altered the expression of TRAF3 (p < 0·05), CD40 (p < 0·001), CLECL1 (p <0·0001), TNFAIP8 (p < 0·001) and TNFRSF1A (p < 0·001). Interpretation: These data suggest that EBNA2 can enhance or reduce expression of the gene depending on the risk allele, likely promoting EBV infection. This is consistent with the concept that these MS risk loci affect MS risk through altering the response to EBNA2. Together with the extensive data indicating a pathogenic role for EBV in MS, this study supports targeting EBV and EBNA2 to reduce their effect on MS pathogenesis

‘Younger People Have Like More of an Imagination, No Offence’: Participant Perspectives on Public Engagement

© 2012, Copyright Taylor & Francis Group, LLC. A wide range of work has reported on the outcomes of public engagement activities and the views expressed by public participants towards specific areas of science and technology. Such work has rarely gone on to explore with public participants their attitudes to the engagement experienced itself, often focusing instead on more practical or quantifiable aspects. This article draws on public participants’ reactions to 11 ‘engagement’ events, occurring across the UK in 2007–2008. Reporting on 33 semi-structured interviews, we focus on their views of participation and engagement in terms of motivations, expectations and expertise. The results suggest that participants have considerable expectations in terms of information and interaction, operate with critical but respectful notions of other ‘publics’ and expertise, and may develop habitual tendencies regarding engagement

Host defense peptides identified in human apolipoprotein B as natural food bio-preservatives: Evaluation of their biosafety and digestibility

The employment of chemical agents in the food industry is raising several concerns by consumers and is leading to an increasing interest in natural food preservatives. Among alternatives, host defense peptides (HDPs) have attracted great interest for their ability to preserve food samples from contamination without altering their quality, taste, and organoleptic properties. Recently, we evaluated the applicability of ApoB-derived peptides as novel food bio-preservatives and demonstrated their ability to prevent chicken meat sample contamination when immobilized on chitosan films. To perform a further step towards the applicability of these peptides in the food field, here we evaluated peptides biosafety and digestibility. To do this, we used a multidisciplinary approach including the evaluation of the peptides' toxicity and antimicrobial activity, the analysis of resistance phenotype development, an in silico prediction of the peptides' susceptibility to proteases and the evaluation of the peptides' stability in simulated gastric and intestinal fluids. ApoB-derived peptides were found to be nontoxic when tested on human gastric carcinoma cells SNU-1 and on human colon-rectal adenocarcinoma cells HT-29, and not to induce resistance phenotype in Salmonella strains. Bioinformatic analyses showed that the peptides are susceptible to several proteases, as also confirmed by experiments in simulated gastric and intestinal fluids. Altogether these findings open interesting perspectives to the future applicability of ApoB-derived peptides as novel food biopreservatives

Public perceptions of, and responses to, desalination in Australia : a report on findings

Desalination as a method of ensuring a rainfall independent source of potable water has become an increasingly favourable option for Australian governments, particularly over the last decade or so. This is especially true for metropolitan areas. The social acceptability of new sources of water, however, impacts on the readiness with which publics accept changed or augmented water supplies. Researchers at Deakin University, Victoria University and Murdoch University, with funding from the National Centre of Excellence in Desalination Australia (NCEDA), have conducted a study of public attitudes to desalination in Australia. This report outlines the results of the 18 month project, which comprises three sections. The cornerstone of the project is a national survey (n=3077), conducted by Datacol Research. The second component of the study consists of focus groups in the vicinity of three existing or developing desalination plants: Wonthaggi (Victoria), Port Stanvac (South Australia), and Kwinana (Western Australia). The third component of the study presented in this report involves interviews with desalination scientists and other technical experts, in relation to their attitudes to communication with lay publics

The interaction of human and Epstein–Barr virus miRNAs with Multiple Sclerosis risk loci

Although the causes of Multiple Sclerosis (MS) still remain largely unknown, multiple lines of evidence suggest that Epstein–Barr virus (EBV) infection may contribute to the development of MS. Here, we aimed to identify the potential contribution of EBV-encoded and host cellular miRNAs to MS pathogenesis. We identified differentially expressed host miRNAs in EBV infected B cells (LCLs) and putative host/EBV miRNA interactions with MS risk loci. We estimated the genotype effect of MS risk loci on the identified putative miRNA:mRNA interactions in silico. We found that the protective allele of MS risk SNP rs4808760 reduces the expression of hsa-mir-3188-3p. In addition, our analysis suggests that hsa-let-7b-5p may interact with ZC3HAV1 differently in LCLs compared to B cells. In vitro assays indicated that the protective allele of MS risk SNP rs10271373 increases ZC3HAV1 expression in LCLs, but not in B cells. The higher expression for the protective allele in LCLs is consistent with increased IFN response via ZC3HAV1 and so decreased immune evasion by EBV. Taken together, this provides evidence that EBV infection dysregulates the B cell miRNA machinery, including MS risk miRNAs, which may contribute to MS pathogenesis via interaction with MS risk genes either directly or indirectly