Independent tuning of acoustic and mechanical properties of phantoms for biomedical applications of ultrasound

In this work the preparation of tissue mimicking materials (TMMs) with independently tunable acoustic and elastic properties is reported. Although a large number of hydrogel, synthetic polymer, polysaccharides or other natural based materials have been proposed and used for the realization of TMMs, both for diagnostic and therapeutic applications of ultrasounds, up to today, simulation of acoustic properties was often performed using solid particles, reducing dramatically the transparency and inevitably affecting the homogeneity and the elastic properties of the TMM. By means of concentrated salts solutions and different polysaccharides, an easy method to prepare these TMMs have been developed. This approach would lead to obtain homogenous TMMs with Young modulus ranging over 3 orders of magnitude, i.e. from 2 to 1500 kPa, with independently tunable attenuation properties. An accurate mechanical and acoustic characterization of these TMMs have been performed. Finally, by means of a preliminary trials on protein denaturation induced by a high focused ultrasound transducer in a transparent TMMs with different attenuation values, the mechanism underlying on the formation and propagation of lesion has been investigated. Obtained results suggest that this 'chemical' approach would strongly support in vitro investigations on the open issues related to diagnostic and therapeutic application of ultrasounds

Future merger of the Milky Way with the Andromeda galaxy and the fate of their supermassive black holes

Our Galaxy and the nearby Andromeda galaxy (M 31) are the most massive members of the Local Group, and they seem to be a bound pair, despite the uncertainties on the relative motion of the two galaxies. A number of studies have shown that the two galaxies will likely undergo a close approach in the next 4-5 Gyr. We used direct N-body simulations to model this interaction to shed light on the future of the Milky Way - Andromeda system and for the first time explore the fate of the two supermassive black holes (SMBHs) that are located at their centers. We investigated how the uncertainties on the relative motion of the two galaxies, linked with the initial velocities and the density of the diffuse environment in which they move, affect the estimate of the time they need to merge and form "Milkomeda". After the galaxy merger, we follow the evolution of their two SMBHs up to their close pairing and fusion. Upon the fiducial set of parameters, we find that Milky Way and Andromeda will have their closest approach in the next 4.3 Gyr and merge over a span of 10 Gyr. Although the time of the first encounter is consistent with other predictions, we find that the merger occurs later than previously estimated. We also show that the two SMBHs will spiral in the inner region of Milkomeda and coalesce in less than 16.6 Myr after the merger of the two galaxies. Finally, we evaluate the gravitational-wave emission caused by the inspiral of the SMBHs, and we discuss the detectability of similar SMBH mergers in the nearby Universe (z <= 2) through next-generation gravitational-wave detectors

Quantum regime of free electron lasers starting from noise

We investigate the quantum regime of a high-gain free-electron laser starting from noise. In the first part, we neglect the radiation propagation and we formulate a quantum linear theory of the N-particle free-electron laser Hamiltonian model, quantizing both the radiation field and the electron motion. Quantum effects such as frequency shift, line narrowing, quantum limitation for bunching and energy spread, and minimum uncertainty states are described. Using a second-quantization formalism, we demonstrate quantum entanglement between the recoiling electrons and the radiation field. In the second part, we describe the field classically but we include propagation effects (i.e. slippage) and we demonstrate the novel regime of quantum SASE with high temporal coherence and discrete spectrum. Furthermore, we describe "quantum purification'' of SASE: the classical chaotic spiking behavior disappears and the spectrum becomes a series of discrete very narrow lines which correspond to transitions between discrete momentum eigenstates ( which originate high temporal coherence)

Mixture distribution modelling of the sensitivities of a digital 3-axis MEMS accelerometers large batch

Huge quantities of low-cost analogue or digital MEMS sensors, in the order of millions per week, are produced by manufacturers. Their use is broad, from consumer electronic devices to Industry 4.0, Internet of Things and Smart Cities. In many cases, such sensors have to be calibrated by accredited laboratories to provide traceable measurements. However, at present, such a massive number of sensors cannot be calibrated and large-scale calibration systems and procedures are still missing. A first step to implementing these methods can be based on the distribution of the sensitivities of the large batches produced. Such distribution is also useful for sensor network end-users who need a single sensitivity, with the associated uncertainty, to be attributed to the whole network. Recently, a large batch of 100 digital 3-axis MEMS accelerometers was calibrated with a primary calibration system developed at INRiM and suitable for 3-axis accelerometers. Distributions of their sensitivities as a function of axis and frequency were analyzed and their non-normal behaviour was shown. However, in the preliminary phase of the study, the calibration uncertainties were not considered in these distributions. Therefore, in this paper, a mixture distribution modelling, based on Monte Carlo simulations and aimed at including the calibration uncertainties in the sensitivity distributions, is implemented and the resulting distributions are compared to the previous ones in histogram form. These distributions are also fitted with Johnson's unbounded and bimodal functions to get continuous distributions. This paper represents a further step towards the development of large-scale statistical calibration methods

Exploratory analysis of transposable elements expression in the C. elegans early embryo

Background: Transposable Elements (TE) are mobile sequences that make up large portions of eukaryote genomes. The functions they play within the complex cellular architecture are still not clearly understood, but it is becoming evident that TE have a role in several physiological and pathological processes. In particular, it has been shown that TE transcription is necessary for the correct development of mice embryos and that their expression is able to finely modulate transcription of coding and non-coding genes. Moreover, their activity in the central nervous system (CNS) and other tissues has been correlated with the creation of somatic mosaicisms and with pathologies such as neurodevelopmental and neurodegenerative diseases as well as cancers. Results: We analyzed TE expression among different cell types of the Caenorhabditis elegans (C. elegans) early embryo asking if, where and when TE are expressed and whether their expression is correlated with genes playing a role in early embryo development. To answer these questions, we took advantage of a public C. elegans embryonic single-cell RNA-seq (sc-RNAseq) dataset and developed a bioinformatics pipeline able to quantify reads mapping specifically against TE, avoiding counting reads mapping on TE fragments embedded in coding/non-coding transcripts. Our results suggest that i) canonical TE expression analysis tools, which do not discard reads mapping on TE fragments embedded in annotated transcripts, may over-estimate TE expression levels, ii) Long Terminal Repeats (LTR) elements are mostly expressed in undifferentiated cells and might play a role in pluripotency maintenance and activation of the innate immune response, iii) non-LTR are expressed in differentiated cells, in particular in neurons and nervous system-Associated tissues, and iv) DNA TE are homogenously expressed throughout the C. elegans early embryo development. Conclusions: TE expression appears finely modulated in the C. elegans early embryo and different TE classes are expressed in different cell types and stages, suggesting that TE might play diverse functions during early embryo development

Dynamics of charge-displacement channeling in intense laser-plasma interactions

The dynamics of transient electric fields generated by the interaction of high intensity laser pulses with underdense plasmas has been studied experimentally with the proton projection imaging technique. The formation of a charged channel, the propagation of its front edge and the late electric field evolution have been characterised with high temporal and spatial resolution. Particle-in-cell simulations and an electrostatic, ponderomotive model reproduce the experimental features and trace them back to the ponderomotive expulsion of electrons and the subsequent ion acceleration.Comment: 5 figures, accepted for publication in New Journal of Physic

Quantum SASE FEL with a laser wiggler

Quantum effects in high-gain FELs are ruled by the quantum FEL parameter, \u3c1 = pmcy/hkr, which is the ratio between the momentum spread at saturation and the one photon momentum recoil. It has been shown that when 3c\u3c1 64 1 the spectrum of the emitted radiation changes from the broad continuous and chaotic spectrum of the classical regime to a series of discrete and equally spaced very narrow lines, due to transitions between discrete momentum states. In this paper we show that the quantum regime can be achieved using Kilometers long magnetic wigglers or a laser wiggler. In this paper we state the scaling laws necessary to operate a Quantum SASE FEL in the Angstrom region with a laser wiggler. Specific example is given having in mind a high power Ti: Sa laser wiggler at \u3bb= 0.8 \u3bcm, in construction at LNF-INFN Laboratories, for the SPARC/PLASMON-X project

The G Protein regulators EGL-10 and EAT-16, the Giα GOA-1 and the Gqα EGL-30 modulate the response of the C. elegans ASH polymodal nociceptive sensory neurons to repellents

<p>Abstract</p> <p>Background</p> <p>Polymodal, nociceptive sensory neurons are key cellular elements of the way animals sense aversive and painful stimuli. In <it>Caenorhabditis elegans</it>, the polymodal nociceptive ASH sensory neurons detect aversive stimuli and release glutamate to generate avoidance responses. They are thus useful models for the nociceptive neurons of mammals. While several molecules affecting signal generation and transduction in ASH have been identified, less is known about transmission of the signal from ASH to downstream neurons and about the molecules involved in its modulation.</p> <p>Results</p> <p>We discovered that the regulator of G protein signalling (RGS) protein, EGL-10, is required for appropriate avoidance responses to noxious stimuli sensed by ASH. As it does for other behaviours in which it is also involved, <it>egl-10 </it>interacts genetically with the G_o/iα protein GOA-1, the G_qα protein EGL-30 and the RGS EAT-16. Genetic, behavioural and Ca²⁺imaging analyses of ASH neurons in live animals demonstrate that, within ASH, EGL-10 and GOA-1 act downstream of stimulus-evoked signal transduction and of the main transduction channel OSM-9. EGL-30 instead appears to act upstream by regulating Ca²⁺transients in response to aversive stimuli. Analysis of the delay in the avoidance response, of the frequency of spontaneous inversions and of the genetic interaction with the diacylglycerol kinase gene, <it>dgk-1</it>, indicate that EGL-10 and GOA-1 do not affect signal transduction and neuronal depolarization in response to aversive stimuli but act in ASH to modulate downstream transmission of the signal.</p> <p>Conclusions</p> <p>The ASH polymodal nociceptive sensory neurons can be modulated not only in their capacity to detect stimuli but also in the efficiency with which they respond to them. The Gα and RGS molecules studied in this work are conserved in evolution and, for each of them, mammalian orthologs can be identified. The discovery of their role in the modulation of signal transduction and signal transmission of nociceptors may help us to understand how pain is generated and how its control can go astray (such as chronic pain) and may suggest new pain control therapies.</p

Characterisation of the secondary-neutron production in particle therapy treatments with the MONDO tracking detector

Particle Therapy (PT) is a non-invasive technique that exploits charged light ions for the irradiation of tumours that cannot be effectively treated with surgery or conventional radiotherapy. While the largest dose fraction is released to the tumour volume by the primary beam, a non-negligible amount of additional dose is due to the beam fragmentation that occurs along the path towards the target volume. In particular, the produced neutrons are particularly dangerous as they can release their energy far away from the treated area, increasing the risk of developing a radiogenic secondary malignant neoplasm after undergoing a treatment. A precise measurement of the neutron flux, energy spectrum and angular distributions is eagerly needed in order to improve the treatment planning system software, so as to predict the normal tissue toxicity in the target region and the risk of late complications in the whole body. The MONDO (MOnitor for Neutron Dose in hadrOntherapy) project is dedicated to the characterisation of the secondary ultra-fast neutrons ([20-400] MeV energy range) produced in PT. The neutron tracking system exploits the reconstruction of the recoil protons produced in two consecutive (n, p) elastic scattering interactions to measure simultaneously the neutron incoming direction and energy. The tracker active media is a matrix of thin squared scintillating fibers arranged in orthogonally oriented layers that are read out by a sensor (SBAM) based on SPAD (Single-Photon Avalanche Diode) detectors developed in collaboration with the Fondazione Bruno Kessler (FBK)