Paleobiology of titanosaurs: reproduction, development, histology, pneumaticity, locomotion and neuroanatomy from the South American fossil record

Fil: García, Rodolfo A.. Instituto de Investigación en Paleobiología y Geología. Museo Provincial Carlos Ameghino. Cipolletti; ArgentinaFil: Salgado, Leonardo. Instituto de Investigación en Paleobiología y Geología. General Roca. Río Negro; ArgentinaFil: Fernández, Mariela. Inibioma-Centro Regional Universitario Bariloche. Bariloche. Río Negro; ArgentinaFil: Cerda, Ignacio A.. Instituto de Investigación en Paleobiología y Geología. Museo Provincial Carlos Ameghino. Cipolletti; ArgentinaFil: Carabajal, Ariana Paulina. Museo Carmen Funes. Plaza Huincul. Neuquén; ArgentinaFil: Otero, Alejandro. Museo de La Plata. Universidad Nacional de La Plata; ArgentinaFil: Coria, Rodolfo A.. Instituto de Paleobiología y Geología. Universidad Nacional de Río Negro. Neuquén; ArgentinaFil: Fiorelli, Lucas E.. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica. Anillaco. La Rioja; Argentin

A large marine eosauropterygian reptile with affinities to nothosauroid diapsids from the Early Triassic of British Columbia, Canada

Sauropterygia, one of the main clades of Mesozoic marine reptiles, diversified shortly after the Permo-Triassic biotic crisis and afterwards remained one of the major components of Early Triassic and later Mesozoic marine ecosystems. On the other hand, actual specimens of marine reptiles of Olenekian age are still rare in the fossil record, coming only from a few localities worldwide. Here we describe associated remains of a larger marine reptile of around 4 m body length, with nothosauroidean affinities from the Sulphur Mountain Formation exposed at the L cirque locality of Wapiti Lake area in British Columbia. Although the specimen records only scattered parts of the posterior vertebral column, some gastral ribs and most notably, the proximal portion of one hind limb together with a fan-shaped ischium, it represents one of the oldest records of Sauropterygia and larger representatives of aff. Nothosauroidea specifically, as well as the northernmost occurrence of such animals in the Triassic. As such, the new specimen is important for understanding the biogeography and early evolution of the group and that of Sauropterygia, in general

The shallow marine placodontCyamodusof the central European Germanic Basin: its evolution, paleobiogeography and paleoecology

The oldest record of Cyamodus is a skull of Cyamodus tarnowitzensis (G\ufcrich 1884, Zt dt Geol Ges. 36:125-144) from Pelsonian shallow marine deposits. During the middle Illyrian, placodonts disappeared from the Germanic Basin. With renewed upper Illyrian transgression, Cyamodus rostratus (M\ufcnster 1839, cber einige ausgezeichnete fossile Fischz\ue4hne aus dem Muschelkalk bei Bayreuth. Birner, Bayreuth, p. 14) appeared which was found in terebratulid shell-rich shallow marine deposits. Abundant remains of Cyamodus muensteri (Agassiz 1839), traditionally referred to as Cyamodus hildegardis and here synonymised with C. muensteri, have been reported from the upper Illyrian/middle Fassanian. Skeletal remains of this species are from the Grenzbitumenzone of the Monte San Giorgio lagoons. The youngest species, Cyamodus kuhnschnyderi (Nosotti and Pinna 1993, Compt Rend Acad Sci Paris. 317:847-850), has been found in the upper Fassanian/lower Longobardian of the southern Germanic Basin or Burgundian Gate when marine facies in the Germanic Basin had nearly disappeared. These successive species provide evidence of monophylogenetic development with a trend towards anterior upper and lower jaw teeth reduction, along with a shortening of the rostrum, over an interval of five million years (243-238 Ma). This evolutionary trend most probably reflects adaptation to specialised feeding on seaplants. The Cyamodus osteoderm carapace was not fused to the vertebral column, and appears to have been primarily a body enhancement that produced neutral or negative buoyancy to facilitate long-period diving

Two new species of the side necked turtle genus, Bairdemys (Pleurodira, Podocnemididae), from the Miocene of Venezuela

The side-necked turtle genusBairdemys (Podocnemididae,Shweboemys Group) from the Miocene of Venezuela and Puerto Rico is revised, and four species are diagnosed on the basis of skull characters; two are de-scribed as new.B. hartsteini Gaffney & Wood, 2002, from the Cibao Formation of Puerto Rico, is characterized by a higher skull, with a straight labial ridge and a premaxillary notch;B. venezuelensis (Wood & Díaz de Gamero 1971), from the Urumaco Formation of Venezuela, is characterized by the absence of a premaxillary notch, a high anterior triturating surface convexity, a deep posterior triturating surface concavity, and a short pterygoid mid-line contact;B. sanchezi, new species, from the Urumaco Formation of Venezuela, is characterized by a very low anterior triturating surface convexity and shallow posterior triturating surface concavity, a premaxillary notch, small size, and extensive temporal and cheek emargination;B. winklerae, new species, from the Urumaco Formation of Venezuela, is characterized by an elongate, narrow snout, with a concave labial ridge, and no premaxillary notch. Based on osteological and bone histological results, an additional strongly crushed skull and associated cara-pace fragment of a previously undetermined podocnemidid from the Urumaco Formation of Venezuela can be further referred toPodocnemis or a closely related taxon — again underscoring the importance of this formation as one of the major fossil lagerstätten of turtles in South America

Angiogenic Biomarkers and Healing of Living Cellular Constructs

The use of intra-oral soft-tissue-engineered devices has demonstrated potential for oral mucosa regeneration. The aim of this study was to investigate the temporal expression of angiogenic biomarkers during wound healing of soft tissue reconstructive procedures comparing living cellular constructs (LCC) with autogenous free gingival grafts. Forty-four human participants bilaterally lacking sufficient zones of attached keratinized gingiva were randomly assigned to soft tissue surgery plus either LCC or autograft. Wound fluid samples were collected at baseline and weeks 1, 2, 3, and 4 post-operatively and analyzed for a panel of angiogenic biomarkers: angiogenin (ANG), angiostatin (ANT), PDGF-BB, VEGF, FGF-2, IL-8, TIMP-1, TIMP-2, GM-CSF, and IP-10. Results demonstrated a significant increase in expression of ANT, PDGF-BB, VEGF, FGF-2, and IL-8 for the LCC group over the autograft group at the early stages of wound repair. Although angiogenic biomarkers were modestly elevated for the LCC group, no clinical correlation with wound healing was found. This human investigation demonstrates that, during early wound-healing events, expression of angiogenic-related biomarkers is up-regulated in sites treated with LCC compared with autogenous free gingival grafts, which may provide a safe and effective alternative for regenerating intra-oral soft tissues (ClinicalTrials.gov number, NCT01134081)

Bone histology of the Middle jurassic turtle shell remains from Kirtlington, Oxfordshire, England

The Middle Jurassic (Bathonian) turtle material from the Mammal Bed at Kirtlington, Oxfordshire, England, has recently been tentatively referred to the Pleurosternidae, although the known synapomorphies of this clade were absent from the specimens. Here we present new evidence from shell bone histology that corroborates reports of pleurosternids at Kirtlington and further reveal that two different histomorphs (= two different taxa) are present in this locality. The first histomorph presents the distinctive histological structure of pleurosternids, which is described herein for the first time: the external cortical bone layers are differentiated into an inner zone of coarse, irregularly interwoven structural fibre bundles and an outer fine-fibred zone. The second histomorph has a more plesiomorphic structure and can only be assigned to Cryptodira indet. A morphological reassessment of the Kirtlington material fails to recognize two different taxa and shows that only sparse evidence supports the presence of pleurosternids in this locality. Shell bone histology thus appears as a powerful tool to study poorly preserved specimens and may in some case (like with pleurosternids) help resolve phylogenetic relationships. According to our results, the stratigraphic appearance of the Pleurosternidae is adjusted from the Kimmeridgian (Late Jurassic) to the Bathonian (Middle Jurassic), which significantly reduces the ghost lineage of Paracryptodir