Fate of plasma membrane during endocytosis. III. Evidence for incomplete breakdown of immunoglobulins in lysosomes of cultured fibroblasts

Rat embryo fibroblasts, when cultured in the presence of control rabbit immunoglobulins (C IgG), doubly labeled by (3)H-acetylation (A) and then conjugated with flourescein (F), take up FAC IgG continuously for at least 72 h. They return the major part of their intake back to the medium in the form of breakdown products of very low molecular weight. Gel filtration and immunological analyses of cells and medium at various times indicate that essentially all the FAC IgG molecules taken up undergo digestion of their Fc part, but that the Fab part of only about three-fourths of the molecules is degraded. The rest remains stored intracellularly in the form of F(ab’)2-type fragments that slowly dissociate into Fab’-type fragments. When FAC IgG was incubated in vitro in the presence of a hepatic lysosomal extract, complete digestion of the Fc part likewise occurred, but the Fab’ part of most if not all the molecules proved resistant to breakdown, and remained as Fab’-type fragments. Cell fractionation experiments have demonstrated that the storage compartment of the FAC IgG and of its digestion residues: (a) shows a density distribution pattern in a sucrose gradient identical to that of the lysosomal marker N-acetyl-β-glucosaminidase and clearly dissociated from that of the Golgi marker galactosyltransferase, and (b) accompanies the lysosomal marker in its density shift induced by exposure of the cells to chloroquine. It is concluded that storage and processing of FAC IgG by rat fibroblasts occur in a single, digestively active compartment of lysosomal nature, and that resistance to digestion of certain Fab’-type fragments accounts largely for the inability of the lysososmal enzymes to completely digest the FAC IgG taken up. This conclusion implies that the intracellular storage compartment through which, in earlier work, plasma membrane patches were found to transit after endocytosis and before recycling to the cell surface consists of authentic lysosomes

Ivory Coast microtektite strewn field: description and relation to the Jaramillo geomagnetic event

During the present study the Ivory Coast microtektite layer was found in cores from five equatorial Atlantic sites, bringing the total number of Ivory Coast microtektite-bearing cores to eleven. The strewn field appears to be restricted to between 9°N and 12°S latitude. There is a general increase in the concentration of microtektites towards the Bosumtwi crater, which is generally thought to be the source of the Ivory Coast tektites. The relationship between the onset of the Jaramillo subchron and the Ivory Coast microtektite layer has been investigated in six cores. A plot of the difference in depth between the base of the Jaramillo subchron and the microtektite layer versus sediment accumulation rate was used to determine the average post-depositional remanent magnetization (PDRM) acquisition depth and the age difference between the onset of the Jaramillo subchron and the deposition of the microtektites. Assuming that the PDRM acquisition depth does not vary with sediment accumulation rate, we find that the average PDRM acquisition depth is 7 cm and that the microtektites were deposited approximately 8 ky after the onset of the Jaramillo subchron. This indicates that the impact responsible for the Ivory Coast tektites and microtektites could not be causally related to the geomagnetic reversal at the base of the Jaramillo subchron

Vertical Drop Of 21-Pwr Waste Package On Unyielding Surface

The objective of this calculation is to determine the structural response of a 21-PWR (pressurized-water reactor) Waste Package (WP) subjected to the 2-m vertical drop on an unyielding surface at three different temperatures. The scope of this calculation is limited to reporting the calculation results in terms of stress intensities in two different WP components. The information provided by the sketches (Attachment I) is that of the potential design of the type of WP considered in this calculation, and all obtained results are valid for that design only

Use of Guided Wave Inspections to Monitor the Integrity of Nuclear Power Station Boilers

This paper describes a ground-breaking application of guided wave testing for the nuclear power industry. Hartlepool and Heysham 1 power stations in the UK contain boilers of a unique ‘pod’ design in which the spiral boiler tubes are supported from above by a central vertical ‘spine’, which is a tubular component approximately 520mm in diameter and 21m long with complex changes in cross-section and attachments along its length. There are 32 boilers in the two stations. The gas which has passed through the reactor core flows down over the boiler tubes, heating the water inside. Only the top 2m of each spine is accessible above the top of the boiler pod. Plant Integrity Ltd was engaged by to inspect these ‘spine’ supports in 2003 using its Teletest guided wave system at statutory outages, which occur on a 3-year interval for each reactor. The Teletest tool was positioned in the only accessible place, just below the inlet header which protrudes approximately 2m from the top of the boiler. A procedure was developed to enable the full 21m length of the spine inside the boiler pod itself to be examined. During one of the regular Teletest inspections, at Heysham 1 power station in September 2013, a significant change in response was detected from the central part of one of the boiler spines. As a result, two of the eight boilers at Heysham 1 Reactor 1 were shut down pending further investigations. The indication was confirmed to be a structurally significant crack in the spine concerned. The crack detected was at the exact position and of the approximate extent reported by Teletest, which had detected the flaw from some 10m away. All four reactors at Hartlepool and Heysham 1 power stations were shut down in the latter part of 2014 while a new safety case for return to service of the remaining boilers was made. There is currently no viable alternative to guided wave inspection for monitoring the integrity of these boiler spines and a significant programme of work has been carried out to demonstrate the performance of Teletest for detection of flaws in these components under a variety of circumstances in order to support the safety case for operation. This has involved theoretical modelling, review of information contained in historical inspection data, practical tests on a full scale mock-up component, development of high temperature Teletest tooling to allow in- service tests and production of automated Teletest data collection equipment to allow real time in-service monitoring of the spines. TWI’s and Plant Integrity’s support enabled all four reactors to be returned to service before the end of 2014

Acute taurine supplementation enhances thermoregulation and endurance cycling performance in the heat

This study investigated the effects of oral taurine supplementation on cycling time to exhaustion at a fixed-intensity and thermoregulation in the heat. In a double-blind, randomised crossover design, 11 healthy males participated in a time to exhaustion test in the heat (35°C, 40% RH), cycling at the power output associated with ventilatory threshold, 2 h after ingesting: Taurine (50 mg kg-1) or placebo (3 mg kg-1 maltodextrin). Core and mean skin temperature, mean sweat rate, heart rate, rating of perceived exertion (RPE), thermal comfort and thermal sensation were measured during exercise and blood lactate concentration (B[La]) was measured after exercise. Taurine supplementation increased time to exhaustion by 10% (25.16 min vs. 22.43 min, p = 0.040), end sweat rate by 12.7% (687 nL min-1 vs. 600 nL min-1, p = 0.034) and decreased B[La] by 16.5% (5.75 mmol L-1 vs. 6.85 mmol L-1, p = 0.033). Core temperature was lower in the final 10% of the time to exhaustion (38.5°C vs. 38.1°C, p = 0.049). Taurine supplementation increased time to exhaustion and local sweating, while decreasing RPE and core temperature in the later stages of exercise, as well as reducing post-exercise B[La]. This study provides the evidence of taurine's role in thermoregulatory processes. These findings have implications for the short-term preparation strategies of individuals exercising in the heat. Based on these findings, a single dose of taurine 2 h prior to training or competition would provide an ergogenic and thermoregulatory effect

Aquilegia, Vol. 31 No. 6, Winter 2007, Newsletter of the Colorado Native Plant Society

https://epublications.regis.edu/aquilegia/1122/thumbnail.jp

Experiences, Opportunities and Challenges of Implementing Task Shifting in Underserved Remote Settings: The Case of Kongwa District, Central Tanzania.

Tanzania is experiencing acute shortages of Health Workers (HWs), a situation which has forced health managers, especially in the underserved districts, to hastily cope with health workers' shortages by adopting task shifting. This has however been due to limited options for dealing with the crisis of health personnel. There are on-going discussions in the country on whether to scale up task shifting as one of the strategies for addressing health personnel crisis. However, these discussions are not backed up by rigorous scientific evidence. The aim of this paper is two-fold. Firstly, to describe the current situation of implementing task shifting in the context of acute shortages of health workers and, secondly, to provide a descriptive account of the potential opportunities or benefits and the likely challenges which might ensue as a result of implementing task shifting. We employed in-depth interviews with informants at the district level and supplemented the information with additional interviews with informants at the national level. Interviews focussed on the informants' practical experiences of implementing task shifting in their respective health facilities (district level) and their opinions regarding opportunities and challenges which might be associated with implementation of task shifting practices. At the national level, the main focus was on policy issues related to management of health personnel in the context of implementation of task shifting, in addition to seeking their opinions and perceptions regarding opportunities and challenges of implementing task shifting if formally adopted. Task shifting has been in practice for many years in Tanzania and has been perceived as an inevitable coping mechanism due to limited options for addressing health personnel shortages in the country. Majority of informants had the concern that quality of services is likely to be affected if appropriate policy infrastructures are not in place before formalising tasks shifting. There was also a perception that implementation of task shifting has ensured access to services especially in underserved remote areas. Professional discontent and challenges related to the management of health personnel policies were also perceived as important issues to consider when implementing task shifting practices. Additional resources for additional training and supervisory tasks were also considered important in the implementation of task shifting in order to make it deliver much the same way as it is for conventional modalities of delivering care. Task shifting implementation occurs as an ad hoc coping mechanism to the existing shortages of health workers in many undeserved areas of the country, not just in the study site whose findings are reported in this paper. It is recommended that the most important thing to do now is not to determine whether task shifting is possible or effective but to define the limits of task shifting so as to reach a consensus on where it can have the strongest and most sustainable impact in the delivery of quality health services. Any action towards this end needs to be evidence-based

Psychological climates in action learning sets: A manager’s perspective

Action Learning (AL) is often viewed as a process that facilitates professional learning through the creation of a positive psychological climate (Marquart, 2000; Schein, 1979). An psychological climate that fosters an environment in which learning set members feel psychologically safe enough to reflect upon both the successes, and failures in their professional life without any form of repercussion. However, there has been little attention given to the ways that that psychological climate develops, and the differing facets that create that climate. In response to such deficit, this paper reports the outcomes of interviews with eleven managers, all of whom are former AL set members on their experiences of action learning set membership. Drawing upon an interpretivist philosophy, the paper explores the key themes that emerged from the analysis of those interviews. The analysis serves to illustrate the differing facets that collectively contribute creation of a positive psychological climate that is conducive for learning. Analysis points to the relative importance of such facets as: trust, honesty, vulnerability, reciprocity, confidentiality and personal disclosure, all of which have the capacity to lead to a positive psychological climate in action learning sets. This paper is useful for developing an understanding of the differing facets in AL sets that create a psychological climate conducive for learning. As such, it has utility for action learning facilitators, set members, academics and educational consultants

Controlled ordering of topological charges in an exciton-polariton chain

We demonstrate, experimentally and theoretically, controlled loading of an exciton-polariton vortex chain into a 1D array of trapping potentials. Switching between two types of vortex chains, with topological charges of the same or alternating sign, is achieved by appropriately shaping an off-resonant pump beam that drives the system to the regime of bosonic condensation. In analogy to spin chains, these vortex sequences realise either a "ferromagnetic" or an "anti-ferromagnetic" order, whereby the role of spin is played by the orbital angular momentum. The ferromagnetic ordering of vortices is associated with the formation of a persistent chiral current. Our results pave the way for the controlled creation of nontrivial distributions of orbital angular momentum and topological order in a periodic exciton-polariton system.PostprintPostprintPeer reviewe

Electrode Polarization Effects in Broadband Dielectric Spectroscopy

In the present work, we provide broadband dielectric spectra showing strong electrode polarization effects for various materials, belonging to very different material classes. This includes both ionic and electronic conductors as, e.g., salt solutions, ionic liquids, human blood, and colossal-dielectric-constant materials. These data are intended to provide a broad data base enabling a critical test of the validity of phenomenological and microscopic models for electrode polarization. In the present work, the results are analyzed using a simple phenomenological equivalent-circuit description, involving a distributed parallel RC circuit element for the modeling of the weakly conducting regions close to the electrodes. Excellent fits of the experimental data are achieved in this way, demonstrating the universal applicability of this approach. In the investigated ionically conducting materials, we find the universal appearance of a second dispersion region due to electrode polarization, which is only revealed if measuring down to sufficiently low frequencies. This indicates the presence of a second charge-transport process in ionic conductors with blocking electrodes.Comment: 9 pages, 6 figures, experimental data are provided in electronic form (see "Data Conservancy"