2,339 research outputs found
PHYCOBILISOMES AND ISOLATED PHYCOBILIPROTEINS. EFFECT OF GLUTARDIALDEHYDE AND BENZOQUINONE ON FLUORESCENCE
The fluorescence of the biliproteins C-phycocyanin from Spirulina platensis, B-phycoerythrin
from Porphyridium cruentum and of isolated whole P. cruentum phycobilisomes is quenched in the
presence of glutardialdehyde (GA) or benzoquinone (BQ). The kinetics of fluorescence decrease thus
induced is biphasic. If GA is used as a quencher, the fluorescence can be recovered at 77 K. Contrary to
the GA-effect, only a minor recovery takes place with BQ at 77K, thus demonstrating a different
mechanism of action of GA and BQ on biliprotein
PHOTOCHEMICAL RING-OPENING IN meso-CHLORINATED CHLOROPHYLLS
Irradiation of 20-chloro-chlorophylls of the a-type with visible light produces long-wavelength shifted photoproducts, which transform in the dark to linear tetrapyrroles (bile pigments). The possible significance for chlorophyll degradation is discussed
Picosecond time-resolved fluorescence of phycobiliproteins
The α- and β-subunits of C-phycocyanin from Mastigocladus laminosus were prepared according to revised procedures. Both subunits are isolated as dimers, which can be dissociated into monomers with detergent mixtures. The fluorescence decay kinetics are similar for the respective monomers and dimers. In no case could they be fitted by only one (α-subunit) or two exponentials (β-subunit) which are predicted by theory for samples with a unique chromophore—protein arrangement containing one and two chromophores, respectively. It is suggested that there exists a heterogeneity among the chromophores of the subunits, which may persist in the highly aggregated complexes present in cyanobacterial antennas
Short to long-range charge-transfer excitations in the zincbacteriochlorin-bacteriochlorin complex: a Bethe-Salpeter study
We study using the Bethe-Salpeter formalism the excitation energies of the
zincbacteriochlorinbacteriochlorin dyad, a paradigmatic photosynthetic complex.
In great contrast with standard timedependent density functional theory
calculations with (semi)local kernels, charge transfer excitations are
correctly located above the intramolecular Q-bands transitions found to be in
excellent agreement with experiment. Further, the asymptotic Coulomb behavior
towards the true quasiparticle gap for charge transfer excitations at long
distance is correctly reproduced, showing that the present scheme allows to
study with the same accuracy intramolecular and charge transfer excitations at
various spatial range and screening environment without any adjustable
parameter.Comment: 5 pages, 2 figures, 1 tabl
EPR, ENDOR, and TRIPLE resonance studies of modified bacteriochlorophyll cation radicals
A series of substituted bacteriochlorophyll molecules, all used in reconstitution experiments of reaction centers of Rhodobacter sphaeroides (Struck et al. Biochim. Biophys. Acta 1991, 1060, 262-270), were characterized by EPR, electron-nuclear double (ENDOR), and electron-nuclear-nuclear triple (TRIPLE) resonance spectroscopy in their monomeric radical cation states. Effects of different substituents at position 3 in the porphyrin macrocycle were considered, especially for two «crosslinks» between plant and bacterial chlorophylls. These are 3-vinylbacteriochlorophyll where the «bacteria» acetyl group at position 3 was substituted by vinyl and 3-acetylchlorophyll where the «plant» vinyl group was substituted by acety
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