Wettability of natural root mucilage studied by atomic force microscopy and contact angle: Links between nanoscale and macroscale surface properties

Organic coatings are considered as main cause of soil water repellency (SWR). This phenomenon plays a crucial role in the rhizosphere, at the interface of plant water uptake and soil hydraulics. Still, there is little knowledge about the nanoscale properties of natural soil compounds such as root-mucilage and its mechanistic effect on wettability. In this study, dried films of pure and diluted natural root-mucilage from Sorghum (Sorghum sp., moench) on glass substrates were studied in order to explore experimental and evaluation methods that allow to link between macroscopic wettability and nano-/microscopic surface properties in this model soil system. SWR was assessed by optical contact angle (CA) measurements. The nanostructure of topography and adhesion forces of the mucilage surfaces was characterized by atomic force microscopy (AFM) measurements in ambient air, using PeakForce Quantitative Nanomechanical Mapping (PFQNM). Undiluted mucilage formed hydrophobic films on the substrate with CA > 90° and rather homogeneous nanostructure whereas the contact angles of diluted samples were < 90°. AFM height and adhesion images displayed incomplete mucilage surface coverage for diluted samples. Hole-like structures in the film frequently exhibited increased adhesion forces. The spatial analysis of the AFM data via variograms enabled a numerical description of such ‘adhesion holes’. The use of geostatistical approaches in AFM studies of the complex surface structure of soil compounds was considered meaningful in view of the need of comprehensive analysis of large AFM image data sets that exceed the capability of comparative visual inspection. Furthermore, force curves measured with the AFM showed increased break-free distances and pull-off forces inside the observed ‘adhesion holes’, indicating enhanced capillary forces due to adsorbed water films at hydrophilic domains for ambient RH (40 ± 2 %). This offers the possibility of mapping the nanostructure of water layers on soil surfaces and assessing the consequences for wettability. The collected information on macroscopic wetting properties, nanoscale roughness and adhesion structure of the investigated surfaces in this study are discussed in view of the applicability of the mechanistic wetting models given by Wenzel and Cassie-Baxter

Untangle soil-water-mucilage interactions with 1H NMR Relaxometry

Mucilage is produced mainly at the root tips and has a high water holding capacity originating from highly hydrophilic gel-forming substances. The objective of the MUCILAGE project is to understand the mechanistic role of mucilage for the regulation of water supply for plants. Our subproject investigates the chemical and physical properties of mucilage as pure gel and in soil. 1H-NMR Relaxometry and PFG-NMR represent non-intrusive powerful methods for quantification of the water distribution and for monitoring of the water mobility in soil pores and gel phases. NMR relaxation of the protons in gel water differs from the one in pure water due to additional interactions with the gel matrix. Mucilage in soil leads to a hierarchical pore structure, consisting of the polymeric biohydrogel network surrounded by the surface of soil particles. The objective of our study is to distinguish in situ water in gel from pore water in a simplified soil system, and to determine quantitatively how the “gel effect” affects relaxation rate and water self-diffusion coefficient in porous systems. For this, we measured the variations of the water mobility in pure chia mucilage under different conditions by using 1H-NMR relaxometry and PFG-NMR. Using model soils, the signals coming from pore water and gel water were distinguished from each other. For this, we fitted the parameters of the equations describing 1H-NMR relaxation in porous systems with our experimental results, in order to describe how the presence of gel in soil affects 1H-NMR relaxation. Out of this knowledge, we proposed a method, which detects in situ the presence of mucilage in soil and characterizes several gel-specific parameters of the mucilage. Finally, we discussed the potential and limitations of 1H-NMR relaxometry for following natural swelling and shrinking processes of a natural biopolymer in soil

Processes governing development of ecotoxicity in clayey and silty soils incubated with olive mill wast water under different temperature and humidity conditions

Olive oil production generates olive mill wastewater (OMW) with a high content in nutrients and phenolic substances. Its application to soil could be a cost-effective solution for recycling. However, the degree of toxic effects of OMW on soil biota is widely unknown and has to be considered when searching for adisposal strategy. The objective of this study was to understand the degradation process of OMW organic matter and its influence on toxic effects as well as soil properties. We hypothesized that OMW toxicity decreases with degradation of its phenolic components. A higher soil biological activity was expected to increase degradation. We incubated a clayey soil and a silty soil with OMW for 60 days under conditions typical for this region in order to simulate the application during various seasons (winter, spring, summer dry, summer wet). Soil respiration, pH, electrical conductivity, total phenolic content as well as anion and cation content, specific ultraviolet absorbance at 254 nm and dissolved organic carbon were measured at ten points of time during incubation. Soils and methanolic soil extracts were tested for ecotoxicity using Lepidium sativum germination and Folsomia candida egg hatching rate. The degradation and transformation of OMW-organic matter was stronger under warm and humid conditions than under cold and dry conditions. It was furthermore enhanced in the clayey soil compared to the silty soil. Most severe ecotoxicological effects were found under summer dry conditions while spring as well as summer wet conditions led to a fast recovery of both germination and hatching. However, the silty soil did not recover to preapplication levels. In the clayey soil, germination parameter were higher than in control after around 30 days suggesting a fertilizing effect. Effects in methanol extracts were higher in all soils and climatic scenarios. Therefore, remobilization of OMW derived toxic compounds has to be considered on a long-term scale. Egg hatching as most sensitive life-cycle parameter of Folsomia candida showed also the same relation to climatic conditions and soil type but was more robust to OMW compared to Lepidium sativum. Environmental conditions as well as soil type are key factors determining degradation of OMW organic matter and OMW derived ecotoxicity. Therefore, spring application (warm and wet) of OMW seems to be a compromise with regard to OMW recycling, OMW occurrence in winter and farmer considerations

Law School Based Incubators and Access to Justice – Perspectives from Deans

At the end of February 2015, law professors, law deans, incubator staff and attorneys, and self-selected others gathered at California Western School of Law for the Second Annual Conference on Law School Incubators and Residency Programs. The incubators that are the subject of this article tend to focus on transition to law practice and access to justice, and some are also working to incorporate technology for the practice of law as a means of enhancing access to justice. As more law schools decide to host, sponsor or offer an incubator, and following our panel discussion at the February 2015 incubator conference, we thought it would be helpful to consider fundamental issues deans grapple with when asked to launch an incubator. This list may not be exhaustive, but it will certainly more quickly advance the conversation, and offers a roadmap of sorts for an incubator business plan

Law School Based Incubators and Access to Justice – Perspectives from Deans

At the end of February 2015, law professors, law deans, incubator staff and attorneys, and self-selected others gathered at California Western School of Law for the Second Annual Conference on Law School Incubators and Residency Programs. The incubators that are the subject of this article tend to focus on transition to law practice and access to justice, and some are also working to incorporate technology for the practice of law as a means of enhancing access to justice. As more law schools decide to host, sponsor or offer an incubator, and following our panel discussion at the February 2015 incubator conference, we thought it would be helpful to consider fundamental issues deans grapple with when asked to launch an incubator. This list may not be exhaustive, but it will certainly more quickly advance the conversation, and offers a roadmap of sorts for an incubator business plan

How does particulate organic matter (POM) swelling affect soil -water interactions and soil structural stability on different scales?

Particulate organic matter (POM), root mucilage and synthetic polymers are swellable polymeric substances (“hydrogels”) which form a three-dimensional polymer network between soil particles. On the one hand, hydrogels can alter soil hydrological properties via their strong influence on water holding capacity and soil wettability. On the other hand, it has been recently shown that the presence of swollen hydrogel structures between soil particles can significantly contribute to soil structural stability. However, until now, only model polymer hydrogels have been used, and the findings still need to be transferred to soils which contain natural swellable organic substances. In this study, we investigated how the swelling of different POM fractions in soil contributes to soil-water-hydrogel interactions and to soil structural stability on different scales. We assumed that the swelling of easily available inter-aggregate POM (frPOM) and occluded intra-aggregate POM (iPOM) differ in their contribution to soil structural stability. For this purpose, we investigated the structural stability and soil-water interactions of a silty sand soil in a 2x2 nested design comprising tilled and non-tilled as well as compost-fertilized and non-fertilized sub-treatments. POM fractions were isolated by soil density fractionation and subsequently characterized for their swelling and water binding properties. Soil-water interactions in terms of water distribution and water mobility were assessed by one- and two-dimensional 1H-NMR relaxometry and pulsed-field-gradient (PFG) NMR. Results from 1H‑NMR measurements were linked with soil structural stability measurements conducted on the micro- and macroscale using soil rheology, wet sieving and crushing tests. On the micro- and macroscale, soil structural stability was higher for compost-fertilized samples than for non-fertilized with different effects of tillage. This was especially related to the presence of frPOM- and iPOM-associated water which revealed a significantly higher viscosity than mineral pore water. On the microscale, frPOM showed the highest contribution to soil structural stability, whereas iPOM predominantly stabilized the soil structure on the macroscale. The relationships suggest that the spatial location and hence the swellability of organic structures in soil could explain the nature of hydrogel-induced soil structural stability

Short-term soil response under plastic mulching in strawberry cultivation

Plastic mulches (PM) are known for increasing soil temperature and retaining soil moisture, suppressing weeds and avoiding ground contact of on-soil growing products. Thus, the use of PM in agriculture has been significantly increased in the last years, with important economic benefits for the farmers. Most studies dealing with PM emphasize the positive effects of this management, yet recent reports have shown that the use of PM is linked to a decrease of SOM content, soil erosion, soil contamination with plastic residues and in some cases to a high mycotoxin production by soil fungi. This questions the sustainability of the intensive use of PM in agriculture. The aim of this study was to assess the short-term modification of soil physicochemical and microbial parameters under PM, compared to no-mulch (NM). The experiment was conducted in a 2016-planted strawberry field. For each management (PM vs. NM) five plots were selected. Cultivation of strawberry in both PM and NM was done in a ridge-furrow system with subsurface irrigation. Samples were collected prior to the planting (T0) and successively at two (T1) and four months (T2) after planting. Different depths were sampled in the ridges (0-10, 10-30 and 30-60 cm) and in the furrows (0-10 and 10-40 cm). The analysis of the quantity and quality of soil organic matter is ongoing and comprised soil physicochemical analysis: pH, electrical conductivity, water content, bulk density and stability, organic C and N and density fractionation. Moreover, soil microbiology was studied via soil microbial carbon and mycotoxin occurrence as indicator of fungal stress. Additionally, temperature, humidity and pH of soil were daily recorded using an in-field installed measuring station. The continuously recorded environmental data showed differences in the temperature patterns between PM and NM, with highest average temperature under plastic. As well, pH and humidity data indicated differences between the treatments

Tajixanthonhydrat und dessen Verwendung zur Behandlung von Tumorerkrankungen

Es werden Tajixanthonhydrat sowie Tajixanthonhydrat-Derivate der allgemeinen Formel I $F1 beschrieben sowie ein Verfahren zu ihrer Herstellung. Tajixanthonhydrat besitzt u. a. eine ausgeprägte Biofilm-inhibierende Wirkung gegen Staphylococcus epidermidis

Biological Surface Coating and Molting Inhibition as Mechanisms of TiO2 Nanoparticle Toxicity in Daphnia magna

The production and use of nanoparticles (NP) has steadily increased within the last decade; however, knowledge about risks of NP to human health and ecosystems is still scarce. Common knowledge concerning NP effects on freshwater organisms is largely limited to standard short-term (≤48 h) toxicity tests, which lack both NP fate characterization and an understanding of the mechanisms underlying toxicity. Employing slightly longer exposure times (72 to 96 h), we found that suspensions of nanosized (∼100 nm initial mean diameter) titanium dioxide (nTiO2) led to toxicity in Daphnia magna at nominal concentrations of 3.8 (72-h EC50) and 0.73 mg/L (96-h EC50). However, nTiO2 disappeared quickly from the ISO-medium water phase, resulting in toxicity levels as low as 0.24 mg/L (96-h EC50) based on measured concentrations. Moreover, we showed that nTiO2 (∼100 nm) is significantly more toxic than non-nanosized TiO2 (∼200 nm) prepared from the same stock suspension. Most importantly, we hypothesized a mechanistic chain of events for nTiO2 toxicity in D. magna that involves the coating of the organism surface with nTiO2 combined with a molting disruption. Neonate D. magna (≤6 h) exposed to 2 mg/L nTiO2 exhibited a “biological surface coating” that disappeared within 36 h, during which the first molting was successfully managed by 100% of the exposed organisms. Continued exposure up to 96 h led to a renewed formation of the surface coating and significantly reduced the molting rate to 10%, resulting in 90% mortality. Because coating of aquatic organisms by manmade NP might be ubiquitous in nature, this form of physical NP toxicity might result in widespread negative impacts on environmental health

Historical Irrigated Meadows at the River Queich, Rhineland-Palatinate

For centuries the meadows at the river Queich in southern Rhineland-Palatinate were irrigated to enhance the quantity and quality of hay. With the introduction of fertili-zers irrigation was stopped in most areas but partly reactivated within the last few years. Today we find a small-scaled mosaic of (non-) irrigated and (un-) fertilized meadows. The Institute for Environmental Sciences Landau at the University of Koblenz - Landau conducts interdisciplinary research on the meadows to investigate the effects of irrigation and the interactions between the involved terrestrial and aquatic systems