15 research outputs found
Fish schooling as a basis for vertical axis wind turbine farm design
Most wind farms consist of horizontal axis wind turbines (HAWTs) due to the
high power coefficient (mechanical power output divided by the power of the
free-stream air through the turbine cross-sectional area) of an isolated
turbine. However when in close proximity to neighbouring turbines, HAWTs suffer
from a reduced power coefficient. In contrast, previous research on vertical
axis wind turbines (VAWTs) suggests that closely-spaced VAWTs may experience
only small decreases (or even increases) in an individual turbine's power
coefficient when placed in close proximity to neighbours, thus yielding much
higher power outputs for a given area of land. A potential flow model of
inter-VAWT interactions is developed to investigate the effect of changes in
VAWT spatial arrangement on the array performance coefficient, which compares
the expected average power coefficient of turbines in an array to a
spatially-isolated turbine. A geometric arrangement based on the configuration
of shed vortices in the wake of schooling fish is shown to significantly
increase the array performance coefficient based upon an array of 16x16 wind
turbines. Results suggest increases in power output of over one order of
magnitude for a given area of land as compared to HAWTs.Comment: Submitted for publication in BioInspiration and Biomimetics. Note:
The technology described in this paper is protected under both US and
international pending patents filed by the California Institute of Technolog
Generation of Lytic Natural Killer 1.1+, Ly-49− Cells from Multipotential Murine Bone Marrow Progenitors in a Stroma-free Culture: Definition of Cytokine Requirements and Developmental Intermediates
We have developed a stroma-free culture system in which mouse marrow or thymus cells, known to be enriched for lymphoid progenitors, can be driven to generate natural killer (NK) cells. Culture of lineage marker (Lin)−, c-kit+, Sca2+, interleukin (IL)-2/15Rβ (CD122)− marrow cells in IL-6, IL-7, stem cell factor (SCF), and flt3 ligand (flt3-L) for 5–6 d followed by IL-15 alone for an additional 4–5 d expanded the starting population 30–40-fold and gave rise to a virtually pure population of NK1.1+, CD3− cells. Preculture in IL-6, IL-7, SCF, and flt3-L was necessary for inducing IL-15 responsiveness in the progenitors because the cells failed to significantly expand when cultured in IL-15 alone from the outset. Although culture of the sorted progenitors in IL-6, IL-7, SCF, and flt3-L for the entire 9–11-d culture period caused significant expansion, no lytic NK1.1+ cells were generated if IL-15 was not added, demonstrating a critical role for IL-15 in NK differentiation. Thus, two distinct populations of NK progenitors, IL-15 unresponsive and IL-15 responsive, have been defined. Similar results were obtained with Lin−, CD44+, CD25−, c-kit+ lymphoid progenitors obtained from adult thymus. The NK cells generated by this protocol lysed the NK-sensitive target YAC-1 and expressed markers of mature NK cells with the notable absence of Ly-49 major histocompatibility complex (MHC) receptors. However, despite the apparent lack of these inhibitory MHC receptors, the NK cells generated could distinguish MHC class I+ from class I− syngeneic targets, suggesting the existence of novel class I receptors