1,349 research outputs found
Solvated dissipative electro-elastic network model of hydrated proteins
Elastic netwok models coarse grain proteins into a network of residue beads
connected by springs. We add dissipative dynamics to this mechanical system by
applying overdamped Langevin equations of motion to normal-mode vibrations of
the network. In addition, the network is made heterogeneous and softened at the
protein surface by accounting for hydration of the ionized residues. Solvation
changes the network Hessian in two ways. Diagonal solvation terms soften the
spring constants and off-diagonal dipole-dipole terms correlate displacements
of the ionized residues. The model is used to formulate the response functions
of the electrostatic potential and electric field appearing in theories of
redox reactions and spectroscopy. We also formulate the dielectric response of
the protein and find that solvation of the surface ionized residues leads to a
slow relaxation peak in the dielectric loss spectrum, about two orders of
magnitude slower than the main peak of protein relaxation. Finally, the
solvated network is used to formulate the allosteric response of the protein to
ion binding. The global thermodynamics of ion binding is not strongly affected
by the network solvation, but it dramatically enhances conformational changes
in response to placing a charge at the active site of the protein
Drivers and potential predictability of summer time North Atlantic polar front jet variability
The variability of the North Atlantic polar front jet stream is crucial in determining summer weather around the North Atlantic basin. Recent extreme summers in western Europe and North America have highlighted the need for greater understanding of this variability, in order to aid seasonal forecasting and mitigate societal, environmental and economic impacts. Here we find that simple linear regression and composite models based on a few predictable factors are able to explain up to 40% of summertime jet stream speed and latitude variability from 1955 onwards. Regression models covering the earlier part of the twentieth century are much less effective, presumably due to decreased availability of data, and increased uncertainty in observational reanalyses. Sea surface temperature (SST) forcings impact predominantly on jet speed, whereas solar and cryospheric forcings appear to influence jet latitude. The cryospheric associations come from the previous autumn, suggesting the survival of an ice-induced signal through the winter season, whereas solar influences lead jet variability by a few years. Wavelet coherence analysis identifies that associations fluctuate over the study period but it is not clear whether this is just internal variability or actual non-stationarity. Finally we identify areas for future research
To blame? The effects of moralized feedback on implicit racial bias
Implicit bias training (IBT) is now frequently provided by employers, in order to raise awareness of the problems related to implicit biases, and of how to safeguard against discrimination that may result. However, as Atewologun et al (2018) have noted, there is very little systematicity in IBT, and there are many unknowns about what constitutes good IBT. One important issue concerns the tone of information provided regarding implicit bias. This paper engages this question, focusing in particular on the observation that much bias training is delivered in exculpatory tone, emphasising that individuals are not to blame for possessing implicit biases. Normative guidance around IBT exhorts
practitioners to adopt this strategy (Moss-Racusin et al 2014). However, existing evidence about the effects of moralized feedback about implicit bias is equivocal (Legault et al 2011; Czopp et al 2006). Through a series of studies, culminating in an experiment with a pre-registered analysis plan, we develop a paradigm for evaluating the impact of moralized feedback on participants’ implicit racial bias scores. We also conducted exploratory analyses of the impact on their moods, and behavioural intentions. Our results indicated that an exculpatory tone, rather than a blaming or neutral tone, did not make participants less resistant to changing their attitudes and behaviours. In fact, participants in the blame condition had significantly stronger explicit intentions to change future behaviour than those in the ‘no feedback’ condition (see experiment 3). These results indicate that considerations of
efficacy do not support the need for implicit bias feedback to be exculpatory. We tease out the implications of these findings, and directions for future research
In situ consolidation of thermoplastic prepreg tape using automated tape placement technology: Potential and possibilities
The key parameters of the in-situ consolidation of carbon fibre reinforced poly-ether-etherketone
(AS4-CF/PEEK) by automated tape placement (ATP) process were investigated by
manufacturing of continuous rings and by laying tape onto pre-consolidated laminates. In
order to establish and understand correlations between the process parameters and
mechanical properties, a number of parametric studies were performed by manufacturing and
testing the interlaminar shear strength, single lap shear strength and fracture toughness
samples. The main process parameters investigated were the compaction force, tape laying
speed and tool temperature. To achieve a uniform heat distribution across the thermoplastic
tape, a new nozzle was designed. Baseline samples were also manufactured using the
autoclave process to provide a comparison for the ATP composites produced. Optical
microscopy was used for investigating the microstructure of composites compared. It was
found that increasing the tool temperature reduced the temperature gradient between the
incoming tape and substrate, resulting in better lap-shear strength and fracture toughness
properties
Simple Statistical Probabilistic Forecasts of the winter NAO
The variability of the North Atlantic Oscillation (NAO) is a key aspect of Northern Hemisphere atmospheric circulation and has a profound impact upon the weather of the surrounding landmasses. Recent success with dynamical forecasts predicting the winter NAO at lead times of a few months has the potential to deliver great socioeconomic impacts. Here, a linear regression model is found to provide skillful predictions of the winter NAO based on a limited number of statistical predictors. Identified predictors include El Niño, Arctic sea ice, Atlantic SSTs, and tropical rainfall. These statistical models can show significant skill when used to make out-of-sample forecasts, and the method is extended to produce probabilistic predictions of the winter NAO. The statistical hindcasts can achieve similar levels of skill to state-of-the-art dynamical forecast models, although out-of-sample predictions are less skillful, albeit over a small period. Forecasts over a longer out-of-sample period suggest there is true skill in the statistical models, comparable with that of dynamical forecasting models. They can be used both to help evaluate and to offer insight into the sources of predictability and limitations of dynamical models
A rhoptry antigen of Plasmodium falciparum is protective in Saimiri monkeys
A non-polymorphic antigen associated with the rhoptry organelles of Plasmodium falciparum has been purified by immuno-affinity chromatography. The antigen, RAP-1 (rhoptry associated protein-1). which is defined by monoclonal antibodies which inhibit parasite growth in vitro, is a multi-component antigen consisting of four major proteins of 80, 65, 42 and 40 kDa and two minor proteins of 77 and 70 kDa. These proteins were electro-eluted from preparative sodium dodecyl sulphate polyacrylamide gels and protected Saimiri sciureus monkeys from a lethal blood-stage infection of P. falciparum malaria. Sera from the protected animals recognized only proteins of the RAP-1 antigen when used to probe a Western blot of total parasite protein extract, confirming that RAP-1 is responsible for eliciting the protective immune respons
Electric field inside a "Rossky cavity" in uniformly polarized water
Electric field produced inside a solute by a uniformly polarized liquid is
strongly affected by dipolar polarization of the liquid at the interface. We
show, by numerical simulations, that the electric "cavity" field inside a
hydrated non-polar solute does not follow the predictions of standard Maxwell's
electrostatics of dielectrics. Instead, the field inside the solute tends, with
increasing solute size, to the limit predicted by the Lorentz virtual cavity.
The standard paradigm fails because of its reliance on the surface charge
density at the dielectric interface determined by the boundary conditions of
the Maxwell dielectric. The interface of a polar liquid instead carries a
preferential in-plane orientation of the surface dipoles thus producing
virtually no surface charge. The resulting boundary conditions for
electrostatic problems differ from the traditional recipes, affecting the
microscopic and macroscopic fields based on them. We show that relatively small
differences in cavity fields propagate into significant differences in the
dielectric constant of an ideal mixture. The slope of the dielectric increment
of the mixture versus the solute concentration depends strongly on which
polarization scenario at the interface is realized. A much steeper slope found
in the case of Lorentz polarization also implies a higher free energy penalty
for polarizing such mixtures.Comment: 9 pages, 8 figure
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