Genetic variation in the cellular response of Daphnia magna (Crustacea: Cladocera) to its bacterial parasite

Linking measures of immune function with infection, and ultimately, host and parasite fitness is a major goal in the field of ecological immunology. In this study, we tested for the presence and timing of a cellular immune response in the crustacean Daphnia magna following exposure to its sterilizing endoparasite Pasteuria ramosa. We found that D. magna possesses two cell types circulating in the haemolymph: a spherical one, which we call a granulocyte and an irregular-shaped amoeboid cell first described by Metchnikoff over 125 years ago. Daphnia magna mounts a strong cellular response (of the amoeboid cells) just a few hours after parasite exposure. We further tested for, and found, considerable genetic variation for the magnitude of this cellular response. These data fostered a heuristic model of resistance in this naturally coevolving host–parasite interaction. Specifically, the strongest cellular responses were found in the most susceptible hosts, indicating resistance is not always borne from a response that destroys invading parasites, but rather stems from mechanisms that prevent their initial entry. Thus, D. magna may have a two-stage defence—a genetically determined barrier to parasite establishment and a cellular response once establishment has begun

Holographic analysis of diffraction structure factors

We combine the theory of inside-source/inside-detector x-ray fluorescence holography and Kossel lines/x ray standing waves in kinematic approximation to directly obtain the phases of the diffraction structure factors. The influence of Kossel lines and standing waves on holography is also discussed. We obtain partial phase determination from experimental data obtaining the sign of the real part of the structure factor for several reciprocal lattice vectors of a vanadium crystal.Comment: 4 pages, 3 figures, submitte

Information and The Brukner-Zeilinger Interpretation of Quantum Mechanics: A Critical Investigation

In Brukner and Zeilinger's interpretation of quantum mechanics, information is introduced as the most fundamental notion and the finiteness of information is considered as an essential feature of quantum systems. They also define a new measure of information which is inherently different from the Shannon information and try to show that the latter is not useful in defining the information content in a quantum object. Here, we show that there are serious problems in their approach which make their efforts unsatisfactory. The finiteness of information does not explain how objective results appear in experiments and what an instantaneous change in the so-called information vector (or catalog of knowledge) really means during the measurement. On the other hand, Brukner and Zeilinger's definition of a new measure of information may lose its significance, when the spin measurement of an elementary system is treated realistically. Hence, the sum of the individual measures of information may not be a conserved value in real experiments.Comment: 20 pages, two figures, last version. Section 4 is replaced by a new argument. Other sections are improved. An appendix and new references are adde

Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)

<p>Abstract</p> <p>Background</p> <p>Anthracnose (<it>Colletotrichum </it><it>gloeosporioides</it>) is a major limiting factor in the production of yam (<it>Dioscorea </it>spp.) worldwide. Availability of high quality sequence information is necessary for designing molecular markers associated with resistance. However, very limited sequence information pertaining to yam is available at public genome databases. Therefore, this collaborative project was developed for genetic improvement and germplasm characterization of yams using molecular markers. The current investigation is focused on studying gene expression, by large scale generation of ESTs, from one susceptible (TDa 95-0310) and two resistant yam genotypes (TDa 87-01091, TDa 95-0328) challenged with the fungus. Total RNA was isolated from young leaves of resistant and susceptible genotypes and cDNA libraries were sequenced using Roche 454 technology.</p> <p>Results</p> <p>A total of 44,757 EST sequences were generated from the cDNA libraries of the resistant and susceptible genotypes. Greater than 56% of ESTs were annotated using MapMan Mercator tool and Blast2GO search tools. Gene annotations were used to characterize the transcriptome in yam and also perform a differential gene expression analysis between the resistant and susceptible EST datasets. Mining for SSRs in the ESTs revealed 1702 unique sequences containing SSRs and 1705 SSR markers were designed using those sequences.</p> <p>Conclusion</p> <p>We have developed a comprehensive annotated transcriptome data set in yam to enrich the EST information in public databases. cDNA libraries were constructed from anthracnose fungus challenged leaf tissues for transcriptome characterization, and differential gene expression analysis. Thus, it helped in identifying unique transcripts in each library for disease resistance. These EST resources provide the basis for future microarray development, marker validation, genetic linkage mapping and QTL analysis in <it>Dioscorea </it>species.</p

Empathy, engagement, entrainment: the interaction dynamics of aesthetic experience

A recent version of the view that aesthetic experience is based in empathy as inner imitation explains aesthetic experience as the automatic simulation of actions, emotions, and bodily sensations depicted in an artwork by motor neurons in the brain. Criticizing the simulation theory for committing to an erroneous concept of empathy and failing to distinguish regular from aesthetic experiences of art, I advance an alternative, dynamic approach and claim that aesthetic experience is enacted and skillful, based in the recognition of others’ experiences as distinct from one’s own. In combining insights from mainly psychology, phenomenology, and cognitive science, the dynamic approach aims to explain the emergence of aesthetic experience in terms of the reciprocal interaction between viewer and artwork. I argue that aesthetic experience emerges by participatory sense-making and revolves around movement as a means for creating meaning. While entrainment merely plays a preparatory part in this, aesthetic engagement constitutes the phenomenological side of coupling to an artwork and provides the context for exploration, and eventually for moving, seeing, and feeling with art. I submit that aesthetic experience emerges from bodily and emotional engagement with works of art via the complementary processes of the perception–action and motion–emotion loops. The former involves the embodied visual exploration of an artwork in physical space, and progressively structures and organizes visual experience by way of perceptual feedback from body movements made in response to the artwork. The latter concerns the movement qualities and shapes of implicit and explicit bodily responses to an artwork that cue emotion and thereby modulate over-all affect and attitude. The two processes cause the viewer to bodily and emotionally move with and be moved by individual works of art, and consequently to recognize another psychological orientation than her own, which explains how art can cause feelings of insight or awe and disclose aspects of life that are unfamiliar or novel to the viewer

Mass Calibration and Cosmological Analysis of the SPT-SZ Galaxy Cluster Sample Using Velocity Dispersion σv\sigma_v and X-ray YXY_\textrm{X} Measurements

We present a velocity dispersion-based mass calibration of the South Pole Telescope Sunyaev-Zel'dovich effect survey (SPT-SZ) galaxy cluster sample. Using a homogeneously selected sample of 100 cluster candidates from 720 deg2 of the survey along with 63 velocity dispersion ($\sigma_v$) and 16 X-ray Yx measurements of sample clusters, we simultaneously calibrate the mass-observable relation and constrain cosmological parameters. The calibrations using $\sigma_v$ and Yx are consistent at the $0.6\sigma$ level, with the $\sigma_v$ calibration preferring ~16% higher masses. We use the full cluster dataset to measure $\sigma_8(\Omega_ m/0.27)^{0.3}=0.809\pm0.036$. The SPT cluster abundance is lower than preferred by either the WMAP9 or Planck+WMAP9 polarization (WP) data, but assuming the sum of the neutrino masses is $\sum m_\nu=0.06$ eV, we find the datasets to be consistent at the 1.0$\sigma$ level for WMAP9 and 1.5$\sigma$ for Planck+WP. Allowing for larger $\sum m_\nu$ further reconciles the results. When we combine the cluster and Planck+WP datasets with BAO and SNIa, the preferred cluster masses are $1.9\sigma$ higher than the Yx calibration and $0.8\sigma$ higher than the $\sigma_v$ calibration. Given the scale of these shifts (~44% and ~23% in mass, respectively), we execute a goodness of fit test; it reveals no tension, indicating that the best-fit model provides an adequate description of the data. Using the multi-probe dataset, we measure $\Omega_ m=0.299\pm0.009$ and $\sigma_8=0.829\pm0.011$. Within a $\nu$CDM model we find $\sum m_\nu = 0.148\pm0.081$ eV. We present a consistency test of the cosmic growth rate. Allowing both the growth index $\gamma$ and the dark energy equation of state parameter $w$ to vary, we find $\gamma=0.73\pm0.28$ and $w=-1.007\pm0.065$, demonstrating that the expansion and the growth histories are consistent with a LCDM model ($\gamma=0.55; \,w=-1$).Comment: Accepted by ApJ (v2 is accepted version); 17 pages, 6 figure

Analysis of Sunyaev-Zel'dovich Effect Mass-Observable Relations using South Pole Telescope Observations of an X-ray Selected Sample of Low Mass Galaxy Clusters and Groups

(Abridged) We use 95, 150, and 220GHz observations from the SPT to examine the SZE signatures of a sample of 46 X-ray selected groups and clusters drawn from ~6 deg^2 of the XMM-BCS. These systems extend to redshift z=1.02, have characteristic masses ~3x lower than clusters detected directly in the SPT data and probe the SZE signal to the lowest X-ray luminosities (>10^42 erg s^-1) yet. We develop an analysis tool that combines the SZE information for the full ensemble of X-ray-selected clusters. Using X-ray luminosity as a mass proxy, we extract selection-bias corrected constraints on the SZE significance- and Y_500-mass relations. The SZE significance- mass relation is in good agreement with an extrapolation of the relation obtained from high mass clusters. However, the fit to the Y_500-mass relation at low masses, while in good agreement with the extrapolation from high mass SPT clusters, is in tension at 2.8 sigma with the constraints from the Planck sample. We examine the tension with the Planck relation, discussing sample differences and biases that could contribute. We also present an analysis of the radio galaxy point source population in this ensemble of X-ray selected systems. We find 18 of our systems have 843 MHz SUMSS sources within 2 arcmin of the X-ray centre, and three of these are also detected at significance >4 by SPT. Of these three, two are associated with the group brightest cluster galaxies, and the third is likely an unassociated quasar candidate. We examine the impact of these point sources on our SZE scaling relation analyses and find no evidence of biases. We also examine the impact of dusty galaxies using constraints from the 220 GHz data. The stacked sample provides 2.8$\sigma$ significant evidence of dusty galaxy flux, which would correspond to an average underestimate of the SPT Y_500 signal that is (17+-9) per cent in this sample of low mass systems.Comment: 15 pages, 7 figure

Cosmological Constraints from Galaxy Clusters in the 2500 square-degree SPT-SZ Survey

(abridged) We present cosmological constraints obtained from galaxy clusters identified by their Sunyaev-Zel'dovich effect signature in the 2500 square degree South Pole Telescope Sunyaev Zel'dovich survey. We consider the 377 cluster candidates identified at z>0.25 with a detection significance greater than five, corresponding to the 95% purity threshold for the survey. We compute constraints on cosmological models using the measured cluster abundance as a function of mass and redshift. We include additional constraints from multi-wavelength observations, including Chandra X-ray data for 82 clusters and a weak lensing-based prior on the normalization of the mass-observable scaling relations. Assuming a LCDM cosmology, where the species-summed neutrino mass has the minimum allowed value (mnu = 0.06 eV) from neutrino oscillation experiments, we combine the cluster data with a prior on H0 and find sigma_8 = 0.797+-0.031 and Omega_m = 0.289+-0.042, with the parameter combination sigma_8(Omega_m/0.27)^0.3 = 0.784+-0.039. These results are in good agreement with constraints from the CMB from SPT, WMAP, and Planck, as well as with constraints from other cluster datasets. Adding mnu as a free parameter, we find mnu = 0.14+-0.08 eV when combining the SPT cluster data with Planck CMB data and BAO data, consistent with the minimum allowed value. Finally, we consider a cosmology where mnu and N_eff are fixed to the LCDM values, but the dark energy equation of state parameter w is free. Using the SPT cluster data in combination with an H0 prior, we measure w = -1.28+-0.31, a constraint consistent with the LCDM cosmological model and derived from the combination of growth of structure and geometry. When combined with primarily geometrical constraints from Planck CMB, H0, BAO and SNe, adding the SPT cluster data improves the w constraint from the geometrical data alone by 14%, to w = -1.023+-0.042

Constraints on the CMB Temperature Evolution using Multi-Band Measurements of the Sunyaev Zel'dovich Effect with the South Pole Telescope

The adiabatic evolution of the temperature of the cosmic microwave background (CMB) is a key prediction of standard cosmology. We study deviations from the expected adiabatic evolution of the CMB temperature of the form $T(z) =T_0(1+z)^{1-\alpha}$ using measurements of the spectrum of the Sunyaev Zel'dovich Effect with the South Pole Telescope (SPT). We present a method for using the ratio of the Sunyaev Zel'dovich signal measured at 95 and 150 GHz in the SPT data to constrain the temperature of the CMB. We demonstrate that this approach provides unbiased results using mock observations of clusters from a new set of hydrodynamical simulations. We apply this method to a sample of 158 SPT-selected clusters, spanning the redshift range $0.05 < z < 1.35$, and measure $\alpha = 0.017^{+0.030}_{-0.028}$, consistent with the standard model prediction of $\alpha=0$. In combination with other published results, we constrain $\alpha = 0.011 \pm 0.016$, an improvement of $\sim 20\%$ over published constraints. This measurement also provides a strong constraint on the effective equation of state in models of decaying dark energy $w_\mathrm{eff} = -0.987^{+0.016}_{-0.017}$.Comment: Submitted to MNRAS Letter