Identification of a novel regulatory mechanism for the disease associated protein, uPAR

Expression quantitative trait loci (eQTLs), as determined through a series of statistical association studies collectively known as genome-wide association (GWA) studies, have provided us with a hypothesis free approach for the investigation into regulatory loci for disease and disease-associated proteins. This has led to the identification of multiple novel gene-disease interactions, especially in the field of respiratory medicine. This review describes the case study of a GWA approach in order to identify eQTLs for the soluble form of the urokinase plasminogen activator receptor (uPAR), a protein associated with obstructive respiratory disease. Molecular and cellular investigations based on the eQTLs identified for this GWA study has led to the identification of a novel regulatory mechanism with implications in the disease processes with which this protein is associated. This highlights the potential of eQTLs defined associations in the identification of novel mechanisms, with implications in disease.peer-reviewe

Symbolic Computation of Polynomial Conserved Densities, Generalized Symmetries, and Recursion Operators for Nonlinear Differential-Difference Equations

Algorithms for the symbolic computation of polynomial conserved densities, fluxes, generalized symmetries, and recursion operators for systems of nonlinear differential-difference equations are presented. In the algorithms we use discrete versions of the Fréchet and variational derivatives, as well as discrete Euler and homotopy operators. The algorithms are illustrated for prototypical nonlinear polynomial lattices, including the Kac-van Moerbeke (Volterra) and Toda lattices. Results are shown for the modified Volterra and Ablowitz-Ladik lattices

Flow interference between groups of three and four equispaced cylinders

This thesis is a study of the interference forces acting on one cylinder comprising a group of three and four cylinders when situated in a free stream flow. The spacing of the cylinders was such that the lines joining their axes formed an equilateral triangle, and a square respectively. The study is split into two parts, (a) potential flow over the groups of cylinders (b) real flow over the groups of cylinders. Bound with copies of the authors' published papers.~Bibliography: pages 132-137

Impact of three inactivated bovine viral diarrhoea virus vaccines on bulk milk p80 (NS3) ELISA test results in dairy herds

peer-reviewedBovine viral diarrhoea virus (BVDV) is endemic in many countries and vaccines are used as a component of control and eradication strategies. Surveillance programmes to detect exposure to BVDV often incorporate the use of bulk milk (BM) testing for antibodies against BVDV p80 (NS3), but vaccination can interfere with these results. The aim of this study was to evaluate whether BVDV vaccines would confound BM testing for specific antibodies in a nationally representative group of commercial dairy farms in the Republic of Ireland. A total of 256 commercial dairy herds were included in the statistical analysis. Quarterly BM or serum samples from selected weanling heifers (unvaccinated homeborn youngstock) were assessed by ELISA for antibodies against the BVDV p80 subunit and whole virus. Wilcoxon ranksum and receiver operating characteristic (ROC) analyses were used to examine differences among groups vaccinated with one of three commercially available inactivated BVDV vaccines. Two of the three vaccines showed evidence of interference with ELISA testing of BM samples. ROC analysis highlighted that one vaccine did not reduce the discriminatory power of the BVDV p80 ELISA for identification of herds with evidence of recent BVDV circulation, when compared with unvaccinated herds; thus, administration of this vaccine would allow uncomplicated interpretation of BM ELISA test results in vaccinated seropositive herds. Seasonal differences in BM antibody results were identified, suggesting that the latter half of lactation is the most suitable time for sampling dairy herds containing predominantly spring calving cows. The results of the present study are likely to prove useful in countries allowing vaccination during or following BVDV eradication, where BM testing is required as part of the surveillance strategy

Assessing Future Flood Risk and Developing Integrated Flood Risk Management Strategies: A Case Study from the UK Climate Change Risk Assessment

As Earth’s climate changes, individual nations must develop adaptation plans to respond to increasing or new climate risks. This study focuses on changing flood risk in England, UK, and examines the policy framework and actions that underpin England’s adaptation from a flood risk management (FRM) perspective. Specifically, the flood risk projections that fed into the UK’s Climate Change Risk Assessment were analysed alongside newly developed FRM adaptation portfolios that modified the flood risk projections to identify the potential of different measures to reduce Expected Annual Damages (EAD). The key findings indicate that: the range of EAD for all flood sources combined is projected to increase by 18–160% by the 2080s depending on the climate change, population growth and adaptation assumptions applied; adopting an enhanced adaptation approach presents an opportunity to manage much of the climate driven change in flood risk, particularly from river flooding; EAD from coastal flood risk shows the greatest increase relative to present day; and surface water flooding will become an increasingly more significant source of flood risk. Interpretation of the results in the context of the policy framework shows how greater coordination and integration of risk managers and interventions is required to improve adaptation planning

Development of lanthanum nickelate as a cathode for use in intermediate temperature solid oxide fuel cells

The performance of lanthanum nickelate, La2NiO4+δ (LNO), as a cathode in IT-SOFCs with the electrolyte cerium gadolinium oxide, Ce0.9Gd0.1O2−δ (CGO), has been investigated by AC impedance spectroscopy of symmetrical cells. A significant reduction in the area specific resistance (ASR) has been achieved with a layered cathode structure consisting of a thin compact LNO layer between the dense electrolyte and porous electrode. This decrease in ASR is believed to be a result of contact at the electrolyte/cathode boundary enhancing the oxygen ion transfer to the electrolyte. An ASR of 1.0 Ω cm2 at 700 °C was measured in a symmetrical cell with this layered structure, compared to an ASR of 7.4 Ω cm2 in a cell without the compact layer. In addition, further improvements were observed by enhancing the cell current collection and it is anticipated that a symmetrical cell consisting of a layered structure with adequate current collection would lower these ASR values further

EXAFS study of nickel exchanged into zeolite Y

EXAFS and near edge spectroscopy were used to monitor changes i n Ni coordination as a function of treatment conditions after aqueous exchange into zeolite Y. Our results suggest that after calcination and dehydration under the conditions of this study, major site occupancy for Ni appears to be in the tri-coordinate exchange sites , and not i n the hexagonal prisms as suggested by previous x-ray diffraction results

CLASH: accurate photometric redshifts with 14 HST bands in massive galaxy cluster cores

We present accurate photometric redshifts for galaxies observed by the Cluster Lensing And Supernova survey with Hubble (CLASH). CLASH observed 25 massive galaxy cluster cores with the Hubble Space Telescope in 16 filters spanning 0.2–1.7 μm. Photometry in such crowded fields is challenging. Compared to our previously released catalogues, we make several improvements to the photometry, including smaller apertures, intracluster light subtraction, point spread function matching and empirically measured uncertainties. We further improve the Bayesian photometric redshift estimates by adding a redder elliptical template and by inflating the photometric uncertainties of the brightest galaxies. The resulting photometric redshift accuracies are dz/(1+z) ∼ 0.8, 1.0 and 2.0 per cent for galaxies with I-band F814W AB magnitudes < 18, 20 and 23, respectively. These results are consistent with our expectations. They improve on our previously reported accuracies by a factor of 4 at the bright end and a factor of 2 at the faint end. Our new catalogue includes 1257 spectroscopic redshifts, including 382 confirmed cluster members. We also provide stellar mass estimates. Finally, we include lensing magnification estimates of background galaxies based on our public lens models. Our new catalogue of all 25 CLASH clusters is available via Mikulski Archive for Space Telescopes. The analysis techniques developed here will be useful in other surveys of crowded fields, including the Frontier Fields and surveys carried out with Javalambre-Physics of the Accelerated Universe Astrophysical Survey and James Webb Space Telescope