4 research outputs found
Чинники ефективності антикризового управління суб'єктами господарювання в економіці України
У статті розглядаються проблеми формування підходів організації антикризового управління суб'єктами господарювання в економіці України. На ґрунті вітчизняного та зарубіжного досвіду й результатів власних досліджень автора запропоновано психологічний тип антикризового менеджера.
(The article is devoted to the problems of forming of approaches of organization of anticrisis management by the subjects of menage in the economy of Ukraine. On the base of domestic and foreign experience and results of own researches of author the psychological type of anticrisis manager is offered.
Aggregation-Induced Fabrication of Fluorescent Organic Nanorings: Selective Biosensing of Cysteine and Application to Molecular Logic Gate
Self-aggregation behavior in aqueous
medium of four naphthalimide
derivatives has exhibited substitution-dependent, unusual, aggregation
induced emission enhancement (AIEE) phenomena. Absorption, emission,
and time-resolved study initially indicated the formation of J-type
fluorescent organic nanoaggregates (FONs). Simultaneous applications
of infrared spectroscopy, theoretical studies, and dynamic light scattering
(DLS) measurements explored the underlying mechanism of such substitution-selective
aggregation of a chloro-naphthalimide organic molecule. Furthermore,
transmission electron microscopy (TEM) visually confirmed the formation
of ring like FONs with average size of 7.5–9.5 nm. Additionally,
naphthalimide FONs also exhibited selective and specific cysteine
amino acid sensing property. The specific behavior of NPCl aggregation
toward amino acids was also employed as a molecular logic gate in
information technology (IT)
Soft-Templated Room Temperature Fabrication of Nanoscale Lanthanum Phosphate: Synthesis, Photoluminescence, and Energy-Transfer Behavior
We herein report a simple and effective
soft template mediated
synthesis protocol for the room temperature preparation of highly
crystalline cerium (Ce<sup>3+</sup>) and terbium (Tb<sup>3+</sup>)
doped lanthanum phosphate (LaPO<sub>4</sub>) nanorods (NRs) and nanoflowers.
Anionic surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/<i>n</i>-alkane soft template was chosen since it forms stable
reverse micelles (RMs). Transmission electron microscopy (TEM) analysis
corroborated successful formation of LaPO<sub>4</sub>:Ce<sup>3+</sup>,Tb<sup>3+</sup> NRs having different aspect ratios (ranging from
2.8:1 to 7.6:1) under varying reaction conditions. The crystalline
nature of the nanomaterials (NMs) was ascertained by X-ray diffraction
(XRD), high-resolution transmission electron microscopy (HRTEM), and
small-area electron diffraction (SAED) studies. Energy-dispersive
X-ray (EDX) and Fourier transform infrared (FTIR) spectroscopy further
verified the elemental existence of the desirably synthesized nanostructures.
Experimental results from thermogravimetric-differential thermal analysis
(TG-DTA) revealed thermal stability of the NRs up to 800 °C.
The prepared NRs exhibit strong yellowish-green photoluminescence
(PL) at 544 nm (<sup>5</sup>D<sub>4</sub> → <sup>7</sup>F<sub>5</sub>) when excited at 270 nm. Furthermore, time-resolved decay
analysis along with steady-state PL measurements indicated an efficient
energy transfer (ET) phenomenon between Ce<sup>3+</sup> and Tb<sup>3+</sup> ions doped in LaPO<sub>4</sub> (LAP) host matrix
Understanding the Effect of Single Cysteine Mutations on Gold Nanoclusters as Studied by Spectroscopy and Density Functional Theory Modeling
Fluorescent metal
nanoclusters have generated considerable excitement
in nanobiotechnology, particularly in the applications of biolabeling,
targeted delivery, and biological sensing. The present work is an
experimental and computational study that aims to understand the effects
of protein environment on the synthesis and electronic properties
of gold nanoclusters. MPT63, a drug target of <i>Mycobacterium
tuberculosis</i>, was used as the template protein to synthesize,
for the first time, gold nanoclusters at a low micromolar concentration
of the protein. Two single cysteine mutants of MPT63, namely, MPT63Gly20Cys
(mutant I) and MPT63Gly40Cys (mutant II) were employed for this study.
The experimental results show that cysteine residues positioned in
two different regions of the protein induce varying electronic states
of the nanoclusters depending on the surrounding amino acids. A mixture
of five-atom and eight-atom clusters was generated for each mutant,
and the former was found to be predominant in both cases. Computational
studies, including density functional theory (DFT), frontier molecular
orbital (FMO), and natural bond orbital (NBO) calculations, validated
the experimental observations. The as-prepared protein-stabilized
nanoclusters were found to have applications in the imaging of live
cells