Influence of multiple cleaning on the detection capabilities of ISFET structures

The method of cleaning the ISFET structures after application of a biological substance was developed. There are few references in the literature to cleaning methods of this type of structure for biological applications, but they are relatively complex and difficult to automate. We decided to use resources commonly available in technological laboratories and methods that could be relatively easily automated, which would enable the full potential of ISFET transistors to be used. During the experiments, both acetone and deionized water were tested. The cleaning method was modified and it was checked whether it is possible to use such a method on one transistor more than once and how it affects the transistor's detection capabilities. We managed to obtain an effective method of cleaning ISFETs from biological substances. This method does not allow for obtaining exactly the same state as the original state of the transistor, but it ensures its correct operation and determining the influence of the tested biological substance on the transistor based on the results

Animal Sciences 52.indb

Abstract: Carbon synthetized by RF PACVD method enhances the activity of antioxidants. The aim of the presented study was to evaluate the reductive activity of antioxidants in the presence of carbon manufactured by Radio Frequency Plasma Activated Chemical Vapor Deposition method (RF PACVD). 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was employed to estimate antioxidants reductive potential. Based on the experimental results, the antioxidant activity of carbon colloid was not con rmed. Nevertheless the reductive activity of antioxidants measured in time manner, in the presence of carbon, was signi cantly elevated comparing to the antioxidants alone. The presented results suggest that the surface of carbon synthetized by RF PACVD method suspended in water, creates more friendly reductive environment for antioxidants with hydroxyl groups (ascorbic acid -AA and butylated hydroxyanisole -BHA) then with sulfhydryl groups (glutathione -GSH)