Genetic variation in the population of three Polish cattle breeds included into the programme of genetic resources protection and Holstein-Friesian breed, estimation on the basis of polymorphism of 24 microsatellite DNA sequences

The study focused on determining and characterizing genetic variation of three Polish cattle breeds [Whiteback (WB), Polish Red (PR) and Polish Black-and-White (PBW)] included in the programme of genetic resources conservation. The obtained results were related to the genetic variation within the Holstein-Friesian (PHF) breed of Black-and-White variety. Overall, 214 alleles were identified within four examined breeds, including 189 in WB, 178 in PR, 168 in PBW and 158 in PHF. Almost 13% of the identified alleles were the specific ones and the majority of them were determined within WB and PR cattle, 10 alleles in each breed. The greatest genetic distance of 0.013 was established between PR breed and PHF. PR and WB cattle were located in the same clad of neighbour-joining tree which proves their distinction from PBW and PHF cattle.Keywords: Local cattle breeds, genetic variation, microsatellite

Assessment of the genetic structure of Central European cattle breeds based on functional gene polymorphism

As many European cattle breeds are considered to be threatened with extinction, and existing genetic variability is increasingly at risk of being irretrievably lost, its assessment is crucial. The aim of the study was to assess the genetic variability of seven breeds of cattle in Central Europe on the basis of polymorphism at the loci of functional genes, encoding β-lactoglobulin (LGB), leptin (LEP), prolactin (PRL), oestrogen receptor alpha (ERα) and growth hormone receptor (GHR). The research was carried out on 290 individuals – 50 Polish White-backed (PW), 50 Lithuanian White-backed (LWB), 50 Polish Red (PR), 50 Lithuanian Red (LR), 22 Carpathian Brown (CB), 18 Ukrainian Grey (UG) and 50 Slovak Pinzgauer (PG). Gene polymorphism was determined by PCR-RFLP. The statistical indicators estimated, i.e. the frequency of alleles and genotypes, observed and expected heterozygosity (HO and HE), F-statistics, gene flow (Nm), and genetic distances, were used to characterize the genetic structure of these cattle breeds. The research demonstrated that the populations analysed have undergone a bottleneck process as a consequence of the rapid decline in the size of individual populations. Owing to the introduction of genetic resources conservation programmes, endangered populations can slowly be restored to a state of genetic balance. Keywords: Genetic variability, Functional genes, Central European cattle breeds, Gene polymorphism, Population genetic structur

Polymorphism of the Melanocortin 1 Receptor ( MC1R) Gene and its Role in Determining the Coat Colour of Central European Cattle Breeds

There are many genes responsible for the appearance of different coat colours, among which the melanocortin 1 receptor gene (MC1R) plays an important role. The aim of the study was to characterize genetic variation in Central European cattle breeds based on polymorphism of the MC1R gene and factors determining their coat colour. The study was conducted on 290 individuals of the following breeds: Polish White-Backed (PW), Lithuanian White-Backed (LW), Polish Red (PR), Lithuanian Red (LR), Carpathian Brown (CB), Ukrainian Grey (UG), and Slovak Pinzgau (SP). Polymorphism at the MC1R gene locus was analysed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using two restriction enzymes: Cfr10I and SsiI. The proportions of alleles and genotypes in the MC1R locus indicates a strong relationship between polymorphism and the coat colour of cattle: The ED allele proved to be characteristic for the breeds with a white-backed coat (PW and LW), while the dominant allele in the red breeds (PR and LR) was E+. It is noteworthy that coat colour in the SP population was determined only by the recessive e allele, which resulted in the formation of a separate clade in the phylogenetic tree