The development of a diatom-based transfer function along the Pacific coast of eastern Hokkaido, northern Japan—an aid in paleoseismic studies of the Kuril subduction zone

This paper provides a dataset to develop a diatom-based transfer function, which is applicable to paleoseismic studies at southwestern Kuril subduction zone, northern Japan. Modern diatom samples were collected from five transects from saltmarshes of Lakes Akkeshi and Onnetoh along the Pacific coast of eastern Hokkaido. The relationships between diatom species and environmental variables were elucidated by canonical correspondence analysis (CCA) and partial CCAs. Partial CCAs associated with Monte Carlo permutation tests show that elevation accounts for a significant portion of the total variance in the diatom data. Therefore, statistically significant transfer functions quantifying the relationship between modern diatom assemblages and elevation were developed using weighted averaging partial least squares and applied to fossil diatom assemblages from Lake Onnetoh. The reconstructed curve of elevations contains five emergence and four submergence events and the transfer functions calculated the amplitude of four of the emergence events to be at least 1 m. The results are consistent with paleoecological data produced by previous studies. If these events represent uplift associated with interplate earthquake and subsidence during an interseismic period along the Kuril subduction zone, transfer functions of eastern Hokkaido can contribute to reconstruction of the recurrence intervals and the amplitude of earthquakes

Structural effects of phosphate groups on apatite formation in a copolymer modified with Ca2+ in a simulated body fluid

Organic–inorganic composites are novel bone substitutes that can ameliorate the mismatch of Young\u27s moduli between natural bone and implanted ceramics. Phosphate groups contribute to the formation of apatite in a simulated body fluid (SBF) and the adhesion of osteoblast-like cells. Therefore, modification of a polymer with these functional groups is expected to enhance the ability of the organic–inorganic composite to bond with bone. Two phosphate groups have been used, phosphonic acid (–C–PO3H2) and phosphoric acid (–O–PO3H2). However, the effects of structural differences between these phosphate groups have not been clarified. In this study, the apatite formation of copolymers modified with Ca2+ and either –C–PO3H2 or –O–PO3H2 was examined. The mechanism of apatite formation is discussed based on analytical and computational approaches. The copolymers containing –O–PO3H2, but not those containing –C–PO3H2, formed apatite in the SBF, although both released similar amounts of Ca2+ into the SBF. Adsorption of HPO42− from –O–PO3H2 in the SBF following Ca2+ adsorption was confirmed by zeta-potential measurement and X-ray photoelectron spectroscopy. The measurement of the complex formation constant revealed that the –O–PO32−⋯Ca2+ complex was thermodynamically unstable enough to convert into CaHPO4, which was not the case with –C–PO32−⋯Ca2+. The formation of CaHPO4-based clusters was found to be a key factor for apatite nucleation. In conclusion, this study revealed that modification with –O–PO3H2 was more effective for enhancing apatite formation compared with –C–PO3H2

Immunohistochemical Localization of the Water Channels AQP4 and AQP5 in the Rat Pituitary Gland

The pituitary gland is composed of the adenohypophysis and neurohypophysis. The adenohypophysis contains endocrine cells, folliculo-stellate (FS) cells, and marginal layer cells, whereas the neurohypophysis mainly comprises axons and pituicytes. To understand the molecular nature of water transfer in the pituitary gland, we examined the immunohistochemical localization of the membrane water channels aquaporin-4 (AQP4) and AQP5 in rat tissue. Double immunofluorescence analysis of AQP4 and S100 protein, a known marker for FS cells, marginal layer cells, and pituicytes, clearly revealed that FS cells and marginal layer cells in the adenohypophysis and the pituicytes in pars nervosa are positive for AQP4. AQP5 was found to be localized at the apical membrane in some marginal layer cells surrounding the Rathke’s residual pouch, in which AQP4 was observed to be localized on the basolateral membranes. These results suggest the following possibilities: 1) FS cells especially require water for their functions and 2) transepithelial water transfer could occur between the lumen of Rathke’s residual pouch and the interstitial fluid in the adenohypophysis through the AQP4 and AQP5 channels in the marginal layer cells

Erosion and Sedimentation During the September 2015 Flooding of the Kinu River, Central Japan

Erosional and sedimentary features associated with flooding have been documented in both modern and past cases. However, only a few studies have demonstrated the relationship between these features and the corresponding hydraulic conditions that produced them, making it difficult to evaluate the magnitude of paleo-flooding. This study describes the characteristics associated with inundation depth and flow direction, as well as the erosional and sedimentary features resulting from the disastrous flooding of the Kinu River, central Japan, in September 2015. Water levels rose rapidly due to heavy rainfall that eventually overtopped, and subsequently breached, a levee in Joso City, causing destructive flooding on the surrounding floodplain. Distinctive erosional features are found next to the breached levee, while depositional features, such as a sandy crevasse-splay deposit are found further away from the breach. The deposit can be divided into three units based on sedimentary facies. The vertical and lateral changes of these sedimentary facies may be the result of temporal and spatial changes associated with flow during the single flooding event. These observations and quantitative data provide information that can be used to reveal the paleohydrology of flood deposits in the stratigraphic record, leading to improved mitigation of future flooding disasters

Analysis of whole Y-chromosome sequences reveals the Japanese population history in the Jomon period

UTokyo FOCUS Press releases "Archaeological mystery solved with modern genetics : Y chromosomes reveal population boom and bust in ancient Japan" https://www.u-tokyo.ac.jp/focus/en/press/z0508_00056.htm

Specific growth inhibitors of Ralstonia solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, and Clavibacter michiganensis subsp. michiganensis

Plant pathogenic bacteria cause huge yield losses in crops globally. Therefore, finding effective bactericides to these pathogens is an immediate challenge. In this study, we sought compounds that specifically inhibit the growth of Ralstonia solanacearum. As a result, we identified one promising compound, 1-(4-bromophenyl)-6-methoxy-2,3,4,9-tetrahydro-1H-β-carboline, which inhibited the growth of R. solanacearum (Rs1002) from a pilot library of 376 chemicals provided from RIKEN. We further obtained its structural analogues and assessed their ability to inhibit Rs1002 growth. Then we identified five compounds, named ralhibitins A to E, that specifically inhibit growth of Rs1002 at >5 μg/ml final concentration. The most effective compounds, ralhibitins A, C, and E completely inhibited the growth of Rs1002 at 1.25 μg/ml. In addition, ralhibitins A to E inhibited growth of Xanthomonas oryzae pv. oryzae but not the other bacteria tested at a final concentration of 10 μg/ml. Whereas, ralhibitin E, besides inhibiting R. solanacearum and X. oryzae pv. oryzae, completely inhibited the growth of X. campestris pv. campestris and the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis at 10 μg/ml. Growth inhibition by these compounds was stable at pH 6–9 and after autoclaving. Because Rs1002 grew in the culture medium in which ralhibitins were incubated with the ralhibitin-insensitive bacteria, the unaffected bacteria may be able to inactivate the inhibitory effect of ralhibitins. These results suggest that ralhibitins might be potential lead compounds for the specific control of phytopathogenic bacteria

Minimal stratigraphic evidence for coseismic coastal subsidence during 2000 yr of megathrust earthquakes at the central Cascadia subduction zone

Lithology and microfossil biostratigraphy beneath the marshes of a central Oregon estuary limit geophysical models of Cascadia megathrust rupture during successive earthquakes by ruling out >0.5 m of coseismic coastal subsidence for the past 2000 yr. Although the stratigraphy in cores and outcrops includes as many as 12 peat-mud contacts, like those commonly inferred to record subsidence during megathrust earthquakes, mapping, qualitative diatom analysis, foraminiferal transfer function analysis, and 14C dating of the contacts failed to confirm that any contacts formed through subsidence during great earthquakes. Based on the youngest peat-mud contact’s distinctness, >400 m distribution, ~0.6 m depth, and overlying probable tsunami deposit, we attribute it to the great 1700 CE Cascadia earthquake and(or) its accompanying tsunami. Minimal changes in diatom assemblages from below the contact to above its probable tsunami deposit suggest that the lower of several foraminiferal transfer function reconstructions of coseismic subsidence across the contact (0.1–0.5 m) is most accurate. The more limited stratigraphic extent and minimal changes in lithology, foraminifera, and(or) diatom assemblages across the other 11 peat-mud contacts are insufficient to distinguish them from contacts formed through small, gradual, or localized changes in tide levels during river floods, storm surges, and gradual sea-level rise. Although no data preclude any contacts from being synchronous with a megathrust earthquake, the evidence is equally consistent with all contacts recording relative sea-level changes below the ~0.5 m detection threshold for distinguishing coseismic from nonseismic changes