Trispalvės našlaitės (Viola tricolor L.) kolekcinių pavyzdžių kaupimas – rūšies išsaugojimo bei vartojimo medicinoje galimybė

Trispalvė našlaitė (Viola tricolor L.) – jau senovėje gydymui vartotas vaistinis augalas. Jos preparatai slopina kvėpavimo organų uždegimą: bronchitą, tracheitą, laringotracheobronchitą, bronchų astmą, palengvina atsikosėjimą; gydo odos ligas: egzemas, seborėją. Liaudies medicinoje trispalvę našlaitę rekomenduojama vartoti esant nemigai, emocinei įtampai; prakaito, šlapimo, tulžies išsiskyrimui skatinti, skausmui malšinti; onkologijoje – pooperacinių navikų ataugimo profilaktikai. Lietuvoje mažėjant natūralių jos augimviečių skaičiui ir plotams, Kauno botanikos sodo Vaistinių augalų kolekciniame augyne našlaitės pradėtos auginti nuo 1994 m., o per 1999–2002 m. sukaupta 11 kolekcinių pavyzdžių iš keturių Respublikos rajonų, tirtas augalų prisitaikymas augti priemolio dirvoje. Natūraliose augimvietėse našlaitės nesikrūmija, išaugina 8–10 kartų mažiau šoninių stiebų ir jų antžeminė masė 20–70 kartų mažesnė. Jų šaknų sistema 1,7–2 karto silpnesnė negu auginamų augalų. Jie labiau kenčia nuo sausrų, jas naikina įvairios kitos žolės, augimvietės nuolat niokojamos žmonių bei gyvūnųWild pansy (Viola tricolor L.) has a history in folk medicine of helping respiratory problems such as bronchitis, asthma, and cold symptoms. The drugs and extracts are prepared from raw material of pansy; it is a component of some prepared antitussives, cholagogues, dermatological medicines, roborants and tonics, alteratives, and anti-phlebitis remedies. Wild pansy is indigenous to or naturalized in large parts of Europe and the Middle East as far as Central Asia, also found through the United States. In the Lithuanian flora wild pansy habitats areas have been fast reducing; this not only limits the availability of the reserves of medicinal raw materials for pharmacy and therapy needs but also causes a menace to survival of species. The reasons of reduction of natural habitats and areas of wild pansy are not only unfavorable meteorological conditions (including summer droughts) but also the competition of different herbs and irrational human activities. The opportunities of preservation of the species wild pansy need to be cultivated and the most exhaustive adaptation research should be performedKauno medicinos universitetasVytauto Didžiojo universiteta

Introduction of Monarda didyma L. in Lithuania and perspective use of raw materials

Biochemijos katedraBotanikos sodasLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

Purification of purple coneflower root hemagglutinin by affinity chromatography

Lecitins or hemagglutinins are non-immune origin glycoproteins, which can bind carbohydrate structures in specific and reversible manner. This particular feature enables lectin purification by affinity chromatography from complex protein samples. Proteins from Echinerea purpurea L. (Moench) roots haven't been investigated precisely, even though this herb is widely used in traditional medicine. Aim of experiment: To develop hemagglutinin from purple coneflower root purification by affinity chromatography. Experiment tasks: 1. Define carbohydrate affinity to hemagglutinins in purple coneflower root protein extracts; 2. Modify affinity matrix for lectin purification from purple coneflower root protein mixture; 3. Purity hemagglutinins from purple coneflower roots. Materials and methods: 1. To ascertain carbohydratte specificity of hemagglutinin component in crude purple coneflower root extract was determined by hemagglutination inhibition reaction in V bottomed microtiter plates. Twofold serial dilutions of crude protein extract were made and the sample was mixed and incubated for 1 h with equal volume of carbohydrate samples: 3 pentoses: D-arabinose, D-ribose, D-xylose, 5 hexoses and their derivates: D-galactose, D-mannose, D-glucose, Acetyl glucosamine, N-Acetyl glucosamine, 2 disaccharides D-lactose, D-maltose. [...]Botanikos sodasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVilniaus universitetas. Biotechnologijos institutasVytauto Didžiojo universiteta

Estraction conditions of proteins from Echinacea Purpurea L. (Moench) roots and leaves

The purple coneflower (Echinacea purpurea L. (Moench)) has been known and used in traditional medicine for decades, but the interest of this plant pharmacological properties isn't decreasing. Previous studies showed hemagglutinating activity of ethanolic extracts from purple coneflower roots and leaves. Nevertheless, information about purple coneflower proteins and their hemagglutinating properties are still not defined. Aim of experiment: To determine the best extraction conditions with highest protein yield and highest hemagglutinating activity. Experiment tasks: 1. Extraction of proteins from coneflower roots and leaves. 2. Determination of extraction conditions for the highest protein yield. 3. Determination of extraction conditions for the highest hemagglutinating activity. Materials and methods: 1. Proteins were extracted out of coneflower leaves (ratio of herb material and extraction buffer - 1:10) and roots (ratio of herb material and extraction buffer - 1:6) in different conditions: A. At 20±2°C for 2 hours in 3 buffers with different pH (Written below); B. At 4±1°C temperature for 2 hours with 5% of protese inhibitor cocktail (Halt™, Thermo Scientific). C: At 4±1°C for 2 hours with 1% of polivynyl polypirolidone, 0,5% of dithiotrethiol and 0,5% of ethylenediaminetetraacetic acid. After extraction proteins were precipitated with 13.3% trichloracetic acid in acetone and 0.2% of β-mercaptoethanol. 2. Protein amount was measured by Bradford assay. 3. Determination of hemagglutinating activity: protein fractions were poured on suspension with 2% trypsin treated rabbit erythrocytes and incubated for 30 min at 20±2°C temperature. Buffers used for extraction: Acetic buffer pH=5.0, Phosphate buffered saline (PBS) pH=7.4, Tris-HCI buffer pH=8.0. [...]Botanikos sodasLietuvos agrarinių ir miškų mokslų centrasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta

Comparative analysis based on protein content in different vegetation periods of the raw material of (Baptisia australis(L.) R. Br ex Ait f. ) roots

Introduction: Baptisia australis (L.) R. Br ex Ait f., is a perennial legume of the Fabaceae family. Roots of this plant accumulate biologically active substances. The main ones are alkaloids, flavonoids, saponins. For example, alkaloid sparteine gives anti-arrhythmic and flavonoids ̶ antiinflammatory effects. Roots also accumulate protein, but it was not quantified. In this research protein concentration was evaluated based on the different vegetation periods of the raw material of Baptisia australis (L.) R. Br ex Ait f. [1]. Materials and methods: Material - fresh Baptisia australis (L.) R. Br ex Ait f. roots collected in Vytautas Magnus University Botanical garden medical herbs section during different vegetation periods ̶ intense growth, butonization, intense flowering, seed maturity. Methods: protein extraction was made using phosphate buffer solution (PBSx10) prepared from Na2HPO4, NaH2PO4, NaCl and deionized water; protein precipitation using ammonium sulfate (AS); resuspension using PBSx1; spectrophotometric (at 595 nm) protein determination by Bradford method [2]; statistical analysis based on SPSS 17.0 and Microsoft Excel. [...]Lietuvos sveikatos mokslų universitetasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta

Comparison of calcium oxalate crystals-raphides, and oxalic acid content in Chamerion angustifolium (L.) Holub during different vegetation phases

ISBN 978-9955-15-517-1Chamerion angustifolium (L.) Holub -willowherb is a flowering perennial plant of the Onagraceae family, which is very promising in medicine and pharmacy practice. Traditionally, willow herb is used in medicine to treat variety of ailments such as benigne prostate hyperplasia, mouth and skin ulcers, burns. One the most common diagnostic anatomical feature of the Chamerion angustifolium (L.) Holub herb is calcium oxalate crystals- raphides, that are formed from oxalic acid [1-4]. The aim of the experiment: to compare calcium oxalate crystals- raphides, and oxalic acid content in Chamerion angustifolium (L.) Holub herb during different vegetation phases - intensive growing, bud, massive blooming. Experiment tasks: 1. Determine calcium oxalate crystals - raphides, content in stalks and petiole of the willow herb during intensive growing, bud, massive blooming phases. 2. To asses oxalic acid concentration of the Chamerion angustifolium (L.) Holub herb in different vegetation phases (intensive growing, bud, massive blooming). 3. To compare raphides and oxalic acid content during willow herb vegetation. Material and methods: [...]Botanikos sodasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta

Evaluation of phenolic compounds, total flavonoids and radical scavenging activity in Lepidium sativum Linn., Lepidium latifolium L. herb collected in different vegetation phases

Biochemijos katedraBotanikos sodasLietuvos sveikatos mokslų universitetasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta

Determination of antimicrobial activity of raw material from Leonurus cardiaca L. and Leonurus sibiricus L

Background: Leonurus cardiaca L. and Leonurus sibiricus L. – are herbaceous perennial plants in the Lamiaceae family. Various preparations of Leonurus L. herb have different antimicrobial activity [1,2]. In this study used medicinal raw: Leonurus sibiricus L. and Leonurus cardiaca L. obtained from Kaunas Botanical Garden of Vytautas Magnus University, Leonurus cardiaca L. - Mažeikiai district, Leonurus sibiricus L. – Mexico. Materials and methods: Leonurus L. medicinal raw was extracted by different solvents. The extraction method of ethanolic and methanolic extracts – percolation. The aqueous extract preparation - dry herb material was added into distilled water and the water was heated until it started boil. Antibacterial activity of ethanolic and methanolic extracts were performed by agar dilution in Petri dishes, aqueous using agar diffusion method. The minimum inhibitory concentration (MIC) was established. Results: Aqueous extracts inhibited Enterococcus faecalis and Candida albicans, methanolic and ethanolic - Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus, Bacillus subtillis, Proteus vulgaris. All of methanolic and ethanolic extracts showed the least MIC value against Bacillus cereus. The lowest MIC value was determined of Leonurus cardiaca L ethanolic extract (Kaunas Botanical Garden of Vytautas Magnus University) - 4,25 mg/mL against Staphylococcus aureus and methanolic extract of Leonurus cardiaca L. (Mažeikiai district) - 5 mg/mL against Klebsiella pneumoniae. Conclusions: Methanolic and ethanolic extracts of Leonurus cardiaca L. showed better antimicrobial activity than methanolic and ethanolic extracts of Leonurus sibiricus L. Plants habitat did not had the significant effect on antimicrobial activityLietuvos sveikatos mokslų universitetas. Medicinos akademijaLietuvos sveikatos mokslų universitetas. Veterinarijos akademija. Mikrobiologijos ir virusologijos institutasVytauto Didžiojo universiteta

Hemagglutinin from Echinacea purpurea L. root separation and identification

[no. VNS17/045]Lectins or hemagglutinins are non-immune origin glycoproteins, which can bind carbohydrate structures in specific and reversible manner. They have big potential for their therapeutical applications such as immunomodulatory, anticancer antibacterial and other activities. Hemagglutinins from Echinarea purpurea L. (Moench) roots haven’t been investigated. The aim of the experiment: To purify and identify hemagglutinins from Echinarea purpurea L. (Moench) roots. Experiment tasks: 1. Purify hemagglutinins from purple coneflower roots; 2. Identify hemagglutinating glycoproteins. Materials and methods: 1. Affinity column with immobilized D-(+)-mannose ligands was equilibrated and unbound proteins were washed out with phosphate buffer saline (PBS) pH 7,4. Hemagglutinins were eluted out of the column with 0.2 M lactose solution in PBS. Following hemagglutinin fraction was collected and checked for hemagglutinating activity. 2. Hemagglutinating active fraction was separated by polyacrylamide gel electrophoresis and immunoblotted for glycosylated proteins. 3. Glycosylated protein bands were identified by liquid chromatography tandem mass spectrometry by sunflower genome database search. Results: 1. Hemagglutinating active proteins from purple coneflower root were separated from non-active proteins and collected into one fraction. 2. Lysin motive (LysM) peptidoglycan binding domain was identified after database search with identity score equal 182.0. Conclusions: 1. Affinity chromatography method was suitable for separation of hemagglutinating active proteins from purple coneflower root crude protein extract. 2. Identified glycoprotein – LysM domain was responsible for the hemagglutinating activityBotanikos sodasLietuvos agrarinių ir miškų mokslų centro Sodininkystės ir daržininkystės institutasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta

Augalų anatomijos ir morfologijos paveikslų rinkinys : metodinė priemonė

Botanikos sodasKauno medicinos universitetasVytauto Didžiojo universiteta