Comparative analysis based on protein content in different vegetation periods of the raw material of (Baptisia australis(L.) R. Br ex Ait f. ) roots

Abstract

Introduction: Baptisia australis (L.) R. Br ex Ait f., is a perennial legume of the Fabaceae family. Roots of this plant accumulate biologically active substances. The main ones are alkaloids, flavonoids, saponins. For example, alkaloid sparteine gives anti-arrhythmic and flavonoids ̶ antiinflammatory effects. Roots also accumulate protein, but it was not quantified. In this research protein concentration was evaluated based on the different vegetation periods of the raw material of Baptisia australis (L.) R. Br ex Ait f. [1]. Materials and methods: Material - fresh Baptisia australis (L.) R. Br ex Ait f. roots collected in Vytautas Magnus University Botanical garden medical herbs section during different vegetation periods ̶ intense growth, butonization, intense flowering, seed maturity. Methods: protein extraction was made using phosphate buffer solution (PBSx10) prepared from Na2HPO4, NaH2PO4, NaCl and deionized water; protein precipitation using ammonium sulfate (AS); resuspension using PBSx1; spectrophotometric (at 595 nm) protein determination by Bradford method [2]; statistical analysis based on SPSS 17.0 and Microsoft Excel. [...]Lietuvos sveikatos mokslų universitetasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta