Peningkatan Keterampilan Mempraktikkan Permainan Bola Voli Pada Mata Pelajaran Penjasorkes Melalui Pendekatan Model Rotating Trio Exchange Siswa Kelas VI SDN Keper Kecamatan Krembung

Berdasarkan hasil observasi di Kelas VI SDN Keper Kecamatan Krembung Kabupaten Sidoarjo, maka diperoleh data rendahnya hasil belajar pada mata pelajaran Pendidikan Jasmani Olahraga dan Kesehatan. Terbukti dari data mean skor 57,11 dan siswa yang dinyatakan tuntas belajar hanya 52,85% dengan standar ketuntasan minimal ditetapkan 75. Diharapkan dengan adanya penerapan pembelajaran kooperatif model Rotating Trio Exchange ini terjadi peningkatan hasil belajar Pendidikan Jasmani Olahraga dan Kesehatan, mean skor meningkat menjadi 75 atau lebih dan siswa yang dinyatakan tuntas belajar mencapai 75% dari keseluruhan jumlah siswa di Kelas VI. Penelitian ini merupakan penelitian tindakan kelas, yang dilaksanakan dalam 3 siklus, tiap siklus terdiri atas 1 pertemuan dan tiap pertemuan dilaksanakan selama 2 jam pelajaran (2 x 35 menit), dan setiap siklus terdiri atas tahap perencanaan, pelaksanaan tindakan, observasi dan refleksi. Adapun teknik pengumpulan data yang digunakan adalah tes, observasi, wawancara dan jurnal. Tujuan yang diharapkan dari penelitian ini adalah meningkatkan hasil belajar Meningkatkan keterampilan permainan bola voli pada mata pelajaran Pendidikan Jasmani Olahraga dan Kesehatan melalui pembelajaran kooperatif model Rotating Trio Exchange Siswa Kelas VI SDN Keper Kecamatan Krembung Kabupaten Sidoarjo. Hasil penelitian menunjukkan bahwa hasil belajar dapat ditingkatkan dengan pembelajaran kooperatif model Rotating Trio Exchange. Hal ini dapat dibuktikan dengan adanya kenaikan nilai rerata kelas dalam setiap siklusnya. Masing-masing siklus I sebesar 73,46, siklus II sebesar 78,85 dan siklus III 84,62. Selain itu peningkatan ini juga didukung dengan adanya peningkatan persentase ketuntasan belajar siswa yaitu pada siklus I sebesar 61,54%, siklus II meningkat menjadi 84,62% dan siklus III meningkat dengan pesat sebesar 96,15%

Respon Proliferasi, Diferensiasi dan Ekspresi C/EBPa Akibat Paparan Quercetin pada Kultur Preadiposit Tikus (Rattus Norvegicus) Strain Wistar Secara In Vitro

Introduction. Obesity is a metabolic syndrome as risk factor to the cardiovascular disease. Obesity occurs caused by adipocyte massive that is started by proliferation and differentiation of preadipocytes that involves transcription factors such as C/EBPα ,PPARγ and SREBP-1. Is known the TNF α and [Ca2+]i Increase when adiponectin decrease at the end of adipocyte differentiation. Therefore it is a deemed treat the obesity through the inhibition of adipocyte proliferation and differentiation using natural resources. Quercetin in due of the flavonoid found in apple,vegetables etc. The aim of this study is to prove the affectivity of quercetin to inhibit proliferation and differentiation of preadipocyte through inhibited of C/EBPα in rat preadipocytes culture method. Methods. Their study was a laboratory experimental. Quercetin was exposed to the preadipocytes human culture, after it was induced by differentiation stimulator the doses quercetin was grouped in: control, 50 µM (Q50), 125 µM (Q125) and 625 µM (Q625) groups. The amount of proliferation and differentiation was descriptive analyzed. The expression of C/EBPα, were identified by Immunocytochemistry and western blotting. Result. Quercetin 100 inhibited the rate of proliferation cell, while quersetin 625 inhibited differentiation in preadipocytes culture. There was a dose dependent to quersetin towards the decrease of PPARγ expression and C/EBPα expression in this preadipocytes culture. Conclusion. It is conclusion that quersetin inhibits proliferation and differentiation preadipocytes culture through the decrease of the expresion of C/EBP α

Optimizing Collagenase, Fetal Bovine Serum, and Insulin to Isolate, Proliferate, and Differentiate Rat Preadipocyte in vitro

BackgroundThe aim of this study is to develop a method for optimal in vitro proliferation and differentiation of rat adipocyte. MethodsPreadipocyte were isolated from omentum of Rattus norvegicus Wistar using Collagenase type I dan II (Sigma). Cells were cultured in M199 culture containing either 0%, 8% or 10% Fetal Bovine Serum (FBS). Insulin were added into the media once the cells attached to the culture plate Proliferating and differentiated cells were counted and analysed by using Oil Red O dan Hematoxylen (HE) staining. Results This study demonstrated that adipocyte could be isolated using Collagenase type I but not type II. The addition of 10% FBSsignificantly increased the number of preadipocyte and differentiation of adipocyte more than those of 8% FBS and without FBS. The timing of FBS addition was best performed on day 8 using 10% FBS. Specific adipocyte staining using Oil Red O revealed thatthere were core lipids in mature adipocyte. ConclusionsCollagenase tipe I could be used to isolate preadipocyte cells. Supplementation of culture media with 8-10% FBS could enhance the in vitro proliferation of preadipocyte. Standard media M199 containing 10% FBS and insulin may provide an environment to differentiate preadipocyte specifically into adipocyte in vitro

Level of Circulating Endothelial Cells and Expression of Nuclear Factor Kappa Beta of Human’s Peripheral Blood Mononuclear Cells in Subjects with Certain Conditions

Aim: to detect the levels of CEC and expression of NFκB in the three groups of subjects with certain conditions. Methods: this study is an exploratory study using human peripheral blood samples. The study subjects comprised three groups, the group of 23 healthy people, a group of 35 people that has one or more risk factors for vascular disease and a group of 15 vascular disease patients (coronary heart disease, diabetes mellitus, stroke). CEC were isolated from peripheral blood mononuclear cells (PBMC). CEC level is identified through the measurement of CD45 and CD146 by flowcytometry method. NFκB expression is recognized by ELISA method (imgenex, USA). Results: the highest average levels of CEC were found in the sick group (28.6%). The highest average expression of NFκB (924.9495) is found in the group with risk factors. The lowest average expression of NFκB and CEC is found in the healthy group. Statistical analysis of ANOVA at the interval confidence of 95% shows a significant difference (p=0.00) levels of CEC and NFκB expression between the healthy group with the group with the risk of cardiovascular disease (CVD) and patients with known CVD. Conclusion: increase of level CEC and NFκB expression has a strong relationship with vascular disease and its risk factors. Key words: circulating endothelial cells (CEC), nuclear factor kappa beta (NFκB), peripheral blood mononuclear cell (PBMC), vascular disease

Study of some biochemical parameters in young men as effected by Ramadan Fasting

The effect of Ramadan fasting on some blood parameters i.e. serum glucose, urea, uric acid, lipids and proteins, were investigated on young men in Ar Rohmah Islamic dormitory. Nineteen normal and healthy students aging between 12-25 years, residing in the Islamic dormitory, voluntarily to participated in the study. Blood samples were obtained from the volunteers on the 1st and 26th day of Ramadan and analyzed for the aforementioned biochemical parameters. A non-significant effect of Ramadan fasting was observed on most of the parameters studied. However, serum urea, triglycerides, total cholesterol and LDL-cholesterol were reduced significantly (p0.05) but remained within the physiological limits. Decrease in blood urea has been attributed to the effect of at least protein and triglycerides intake to increase lipolytic effect. The reduction in serum cholesterol and LDL is a beneficial effect of Ramadan fasting. The results of the study indicated thatRamadan fasting is quite safe for normal healthy adults

Tarqiatu maharatil kitabah bistiratijiah Think Talk Write fii fasli 'ashir bi MAS Darun Najah Pidie

يعتقد الباحث على الملاحظة أن المعلم في MAS Darun Najah Pidie يعلم اللغة العربية دون استخدام الطريقة الخاصة أو الوسائل الجذابة ويقتصر بحفظ النصوص. لذلك يشعر الطلبة ولم يكونوا قادرين على يعتبر أفكارهم والكتابة اللغة العربية. وأيضا يشعرون بملل أثناء تعلم ولاتكون عندهم الحماسة والدوافع القوية لثورة الكتابة وتطبيق على تعبير عن المعرفة في اللغة العربية. وأما أغراض التأليف في هذه الرسالة فهي التعرف على استعمال استراتيجية Think Talk Write يؤثر الطلبة لترقية مهارة الكتابة لدى الطلاب MAS Darun Najah Pidie ويستعمل الباحث نتيجة الماؤوية والمعدلة لتحليل بيانات الاستيجابة وتحليل ت- test لحصول على التأثير.وأما منهج البحث الذي استعمله الباحث في هذه الرسالة فهو منهج تجرببية بالتصميمات التمهيدية One group pre-test, post-test design)). ولجمع البيانات استعمل الباحث دليل الإختبار ت- (t-test). وأخذ الباحث العينة هي في الفصل الأول ععدهم 30 الطلبة. وأما نتائج المحصولة فهي إنّ استراتيجية Think Talk Write تأثير لترقية مهارة الكتابة وتأثيرها كبيرة (large)

Peran Puerarin Terhadap Aktivitas Intra Dan Ekstraseluler Pada Kultur Human Umbilical Vein Endothelial Cells (HUVECs) Pasca Induksi Leptin

Beberapa penelitian terkini menyebutkan bahwa leptin merupakan salah satu penyebab disfungsi endotel yang merupakan salah satu penyebab aterogenesis. Antioksidan puerarin diduga memiliki kemampuan untuk mencegah mekanisme aterogenesis yang distimulasi oleh beberapa sitokin. Berdasarkan hal tersebut, maka tujuan penelitian ini adalah membuktikan dan mengetahui potensi puerarin untuk menghambat ekspresi dan aktivitas intra dan ekstraseluler VCAM-1, PPAR-γ, SOD dan H2O2, apoptosis dan nekrosis pada kultur Human Umbilical Vein Endothelial Cells (HUVECs) yang diinduksi 25 ng ml-1 leptin. Penelitian ini mempergunakan sel kultur primer HUVECs yang dibagi menjadi empat kelompok perlakuan, yaitu kelompok 0 ng ml-1 dan 0 μM puerarin, kelompok sel yang diinduksi 25 ng ml-1 leptin selama 12 jam, kelompok induksi puerarin 5, 25, 200 dan 525 μM puerarin selama enam jam tanpa leptin, kelompok induksi leptin dan puerarin dengan konsentrasi 5, 25, 200 dan 525 μM selama enam jam. Aktivitas VCAM-1 dan PPAR-γ diketahui dengan analisis imunositokimia, metode ELISA digunakan untuk analisis aktivitas SOD dan H2O2. Apoptosis dan nekrosis sel dianalisis setelah HUVECs diberi penanda BrdU selama 20 jam. Data dianalisis dengan analisis satu jalur (ANOVA) dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa induksi 25 ng ml-1 dapat meningkatkan ekspresi VCAM-1 (2,68 ± 0,15)% dibandingkan dengan perlakuan 0 ng ml-1 (0,54 ± 0,15)%. Perlakuan induksi puerarin 5, 25, 200, 525 μM memberikan dampak negatif terhadap ekspresi VCAM-1 meskipun pengaruh ini tidak signifikan. Puerarin dapat menekan apoptosis dan nekrosis sel, 525 μM puerarin secara efektif dapat menekan ekspresi PPAR-γ. Puerarin tidak memberikan dampak yang signifikan terhadap aktivitas ekstraseluler berdasarkan hasil analisis aktivitas SOD dan H2O2

Ratio of EPC and CEC as Endotel Dysfunction Predictor in High Risk Group with Farmingham Risk Score 10 Years Category

Research background. Circulating Endothelial Cells (CEC) is a reflection of endothelial damage or endothelial stress, increasing of CEC amount depend on endothelial mechanism, edothelial adhesivity damage and cellular apoptosis as a result of decreasing sitoskleleton function. If higher exposure affects the increasing of CEC amount,VEGF other growth factor mediators will be reflected as endothelial stress manifestation which roles in the increasing of re-population and Endothelial Progenitor Cells (EPC) differentiation. Endothelial Progenitor Cells is a mononuclear cell (a part of stem cell) that could change to be mature endothel and roles in re-edothelialisation and neovascularisation. This research aimed to investigate the ratio of EPC : CEC in risk group through Framingham Risk Score (FRS) 10 years approach as endothelial dysfunction predictor. Research method and result. There were 55 research subjects whom taken by FRS scoring and devided into some risk groups and two control groups. They were control group I (health) and control group II (sick). Base blood was taken to every each of them to analyze their EPC and CEC with Flowcytometry. EPC was analyzed by CD34 Per CP Santa Cruz SC-19621 and CD 133 FITC (fluorescein isothiocyanate) Bioss bs-0395R-FITCmarker.WhileCEC was analyzed CD45 FITC Biolegend 202205 dan CD 146 PE Biolegend 134704 marker. Result showed, there was significant ratio differences of EPC : CEC in those six groups which was proven by p-value 0.032< ? (0.05). The higher ratio was in high risk group (139.06). Conclusion. Research showed that EPC amount was increase related to the increasing of high risk level according to FRS 10 years, but its increasing did not followed by its ability to homing in injury area as role part in re-endothelialisation process. It found that EPC amount was higher in high risk group than in low risk group

The Role Of Lycopene to Nuclear Factor Kappa Beta (Nf-κB) Activities and Intracellular Cell Adhesion Molecule-1 (Icam-1) Expressions on Leptin-Induced Endothelial Cell

There is adipocytokine dysregulation on obesity such as amplified leptin, and reduced adiponectin at serum levels . Leptin has been proved to increase oxidative stress in endothelial cell. The adipocyte functions as an important secretory organ via nuclear factor-κ B (NF-κB) releasing a number of bioactive molecules such as leptin. Lycopene, an antioxidant, is presumed having the ability to block the atherogenesis mechanism, which is stimulated a proinflamatory cytokine and adhesion molecules ICAM-1 by NF-κB. Therefore, the aim of this research was to prove and to determine whether lycopene could decrease the NF-κBandICAM-1 expressionin Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by 500 ng/mL leptin. In vitro study used primary culture of the HUVECs were devided in to 7 groups, there were (1) 0 ng/mL leptin and 0 μM lycopene, (2) induced by 500 ng/mL leptin for 12 hours, (3) induced by leptin and lycopene with concentration 10; 25; 40; 55 and 75 μM for 12 hours. Then the identification of NF-κB was applied by using imunocytochemistry compared with ELISA procedure on cell endothel culture lysate and ICAM-1 expression was measured by using RT PCR. It was showed that lycopene 25 μM decreased NF-κB level and ICAM-1 expression significantly in Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by leptin 500 ng/mL.Leptin was increased NF-κB and ICAM-1 expression in HUVECs culture and was decreased by lycopene. Optimum dose of lycopene is 25 μM