QUALITY OF CHILLED CANINE SEMEN IN TRIS-EGG YOLK EXTENDER SUPPLEMENTED WITH SERICIN

                                                                 ABSTRACTThe objective of this research was to evaluate the quality of chilled canine semen in Tris-egg yolk (TEY) extenders containing different concentrations of sericin. Semen were collected from four dogs by massage method. Canine semen was collected using sterile urine pots and evaluated. Sperm-rich fractions were pooled and divided into four equal aliquots, which were then diluted with TEY extenders supplemented with different concentrations of sericin (0%, 0.1%, 0.25%, 0.5%). The diluted semen aliquots were preserved at 4 ℃ in sterile centrifuge tubes and were then evaluated for spermatozoa motility, viability, plasma membrane integrity and acrosome integrity every 12 hours up to 72 h. The TEY extenders supplemented with 0.25% and 0.5% sericin resulted in higher spermatozoa motility and viability at 72 h compared to other TEY extenders (P0.05). The integrity of plasma membrane and acrosome of spermatozoa showed no significant differences among the groups extenders at 72 h. In conclusion, sericin in concentration of 0.25% and 0.5% were able to prevent the motility and viability of canine spermatozoa after storage for 72 h

PCS-4 In Vivo Embryo Production at Cipelang Livestock Embryo Centre

Livestock Embryo Center/Balai Embrio Ternak (BET) Cipelang is one of the government institution under the supervision of Directorate General of Livestock and Animal Health Services, Ministry of Agriculture of the Republic of Indonesia. BET Cipelang has the main task of carrying out the production, development, and distribution of livestock embryo for all regions of Indonesia. BET Cipelang produced embryos using two methods, there are in vivo and in vitro. The produced embryos will be transferred to the recipient cow using embryo transfer technique.                Embryo transfer biotechnology can be used to enhance genetic improvement and to increase marketing opportunities with purebred cattle. Because of their relatively low reproductive rate and long generation interval, embryo transfer is especially useful with cattle. The success of embryo transfer depends on factors associated with the embryo, the recipient or an interaction among the factors of the embryo and recipient [1].                 Embryo quality is one of the main factors for successful embryo production. In vivo embryo production has better quality than in vitro such as cell number, morphology, growth and development ability and after freezing.  The frequency of occurrence of chromosomal abnormalities in in vivo-derived embryos are lower than in vitro and the consequence is that only 30-40% of oocytes resulting from in vitro maturation develop into blastocysts after in vitro fertilization and the in vitro yield rate of embryos are lower than from in vivo [2]. Recently, there are no studies about the performance of in vivo embryo production at BET Cipelang. The aim of this study is to explore the quality of in vivo embryos produced by BET Cipelang

PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES

This study aimed to determine the effect of age on bulls’ productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, ≥ 10 yo with high semen rejection (≥10 HR), and ≥ 10 yo with low semen rejection (≥10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of ≥10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in ≥ 10 yo groups. The ≥10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of ≥10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in ≥10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen

Metode nephrosplenic entrapment reposition untuk penanganan kasus large colon displacement pada kuda

Large colon displacement merupakan perpindahan posisi kolon menjadi ke arah kiri disebut left dorsal displacement of colon (LDDC), atau ke arah kanan disebut right dorsal displacement of colon (RDDC). Perpindahan ini menyebabkan kolik pada kuda. Palpasi per rektal pada kuda G. Galunggung menunjukkan adanya perubahan konsistensi dan perpindahan lokasi kolon sehingga dugaan utama adalah terjadinya large colon displacement. Palpasi per rektal daerah nephrosplenic menunjukkan limpa pindah ke arah caudal di dinding kiri abdomen. Hal ini menunjukkan bahwa kuda G. Galunggung mengalami LDDC. Reposisi mekanis metode nephrosplenic entrapment reposition menggunakan katrol dilakukan untuk mengembalikan posisi kolon yang terperangkap di antara ginjal dan limpa setelah kuda G. Galunggung dibius terlebih dahulu. Konfirmasi secara palpasi per rektal menunjukan bahwa metode tersebut berhasil mengembalikan posisi kolon pada kasus kuda G. Galunggung. Setelah 3 hari perlakuan, kuda makan dan minum secara normal, konsitensi feses normal, serta tidak terjadi kolik maupun ambruk

REVIEW: SOLUTIONS FOR GRAND CHALLENGES IN GOAT AND SHEEP PRODUCTION INDUSTRY

Goats and sheep are valuable as they are a source of meat, milk, fleece, and other products. These livestock are also important both for agriculture and biomedical research. However, the efficient, sustainable, and profitable production of these small ruminants faces major obstacles. Hence, this review analyzes these major challenges specifically, their negative impacts on the industry, and suggests some science-based solutions to overcome them. Those challenged areas are education and training, research, translational research/biotechnology, goat and sheep health, and maintenance of an economically sustainable agribusiness. The suggested solutions include the effective teaching of goat and sheep science to the next generation and public empowerment, support for innovative and translational research, disease prevention and treatment, support for technology transfer, and development of sound agribusiness practices. This review is helpful particularly for scientists, students, and the goat and sheep producers. In general, these information on the current state of goat and sheep agriculture will also help the public to better understand and appreciate the challenges met and opportunities provided in small ruminant production enterprises