ダイガク ジョセイ キョウショクイン ノ ケツエキ ソセイ ニアタエル 3カゲツカン ノ テイ キョウド ユウサンソセイ ジキュウ ウンドウ オ チュウシントシタ フィトネス プログラム ノ カイニュウ コウカ ニカンスル ツイセキ ホウコク

The purpose of the present report is to research the interventional influence of low intensity aerobics exerciseon the blood profile， especially total cholesterol， high－density lipoprotein（HDL）cholesterol and triglyicerides．The exercise intense was approximately 4090 heart rate maximum by Calbournen method． The subjects were thirtyfour voluntary female university staff and mean age was 36．6 years old． The intervention frequency was threetimes a week during three months． The original QoL（Quality Of Life）Questioner， personal exercise check，personal active quantity everyday were put into practice before and after the intervention． The following results were obtained． 1）The body fat percentage was elevated by O．8 per cent significantly． 2）The blood profile of lipids was of no significant difference between pre and post intervention． A little possibility existed， however， that total cholesterol value was enhanced． 3）There was no significant relationship between the active quantity／frequency and the difference in the blood profile in lipids． 4）Reduced group in total cholesterol was with higher total cholesterol value and higher high－density cholesterol value before the intervention than increased group in total cholesterol． Increased group in total cholesterol practiced weight training more frequently than reduced group in total cholesterol and was increased in high－density cholesterol value． 5）Increased group in triglycerides had more triglycerides before the intervention than decreased group in triglycerides． 6）Enhanced group in high－density cholesterol had the lower total cholesterol value before the intervention than reduced group in high－density cholesteroL The total cholesterol value of enhanced group in high－densitycholesterol was also enhanced． And enhanced group in high－density cholesterol had less frequency thanreduced group in high－density cholesterol in the habit of eating irregular meals．論考(Original

テニス キョウギ ニオケル スピード ジキュウセイ ヒョウカ ノタメノ フィールド テスト ニカンスル イチコウサツ

In recent years tennis rallies have increased in speed. Furthermore, several recent cases have been described in which mean blood lactate levels approached the onset of blood lactate accumulation (OBLA). Defining contemporary tennis as an intermittent higIn recent years tennis rallies have increased in speed Furthermore, several recent cases\u272】131 have beendescribed in which mean blood lactate levels approached the onset of blood lactate accumulation (OBLA).Defining contemporary tennis as an intermittent high intensity exercise, the authors aimed to determine a methodof evaluating the physical ability of speed endurance that tennis athletes are required to acquire at present. Thepurpose of this study was firstly to elucidate the optimum form of intermittent exercise for use as field test fortennis players, and secondly to clarify the relationships among speed endurance, ventilatory threshold (VT : %VChmax) and post-exercise blood lactate level.All subjects were tennis athletes (42 males and 34 females, ranging in age from 12 to 21 years) who competedat the university, regional (state), or national junior level. The physical tests evaluated were as follows :intermittent 5 m, 10 m and 40 m sprint runs, and intermittent 10 m shuttle runs, 10 m repeated double shuttle runsand intermittent 50 m change-of-direction runs. These exercises were performed as intermittent exercises andwere performed 10 times with 20-second intervals. Twelve-minute-runs, shuttle-stamina-test (10叩shuttle runsin 3 minutes), 10 sessions of intermittent pedaling (0.075% weight kp/5 second) with 20-second intervals, andtreadmill measurement of VT were also performed. Posトexercise blood lactate was sampled after the intermittentshuttle runs and the intermittent pedaling. Each series, composing 10 trials of the above-mentioned intermittentexercises, was divided into 4 phases : first phase (Isl trial), primal phase (2 to 4山trial), middle phase (5 to 7山trial), and final phase (81 to \u27th trial). The extent to which performance time in the final phase was prolonged incomparison to the previous phases was regarded an indicator of speed endurance

ダイガクセイ ノ セイシンテキ ケンコウド ト ライフ スタイル トノ カンケイ

The life style of the university students has been varing with the rapid transition of social economy and the trasient variation of culture lately． And it was quite possible that such abrupt variations exerted an ill infuluence on the mental and phyical health and the social life of the students． It was so serious a problem that it was important to clear the actual state of the usual life style and the subjective state of health， and then to evidence the relationship between them． On the one side， it was well known that the factors affecting the mental health， besides the life style， were the demographic fact（sex distinction， age， social and economical state），biological charactaristic（physical condition， clinical history， physique， physical fitness and so forth）， creed，estimation of value and so on． However， little work was designed in the subject of university students to elucidate the factors corelated with the health status（GHQ：The General Health Questionnaire）． Therefore， the purpose of the present paper is to reveal the health status and the life style of the students in the Gakushuin university and to the throw some light on the remarkable factors related to the mental health status． The questionnaire was put into operation to the subject of the students taking alesson in physical practise（The health and sports science I，named in this university）late in november，1996． The subjects were the first－year students belonging to the department of literature or science． The recovered data were presupPosed to be the representative of the first－year students this university， because they were required to study the lesson in physical practise． The 28－item General Health Questionnaire was utilized in order to detect the status of mental health． In addition， several articles were supplemented on the life style， fundamental attribution， creed， estimation of value and so forth with reference to the preceding Papers．As the index of the physical charactaristic（physique）， height and weight and BMI（Body Mass Index：weight（kg）／Height（m）2）were measured under the regular physical checkup in April，1996． The outcome of the physical strength and exercise examination fixed in September yearly was applied to the charactaristic of physical fitness． The score of Harvard－Step－Test was applied to the index of the aerobic work capacity（ general physical endurance）． Medicine－ball－throw and Standing－broad－jump were measured to the index of the anaerobic work capacity（power）． Side－step was to the index of the agility． As a result of statistical analysis by means of the quantification theory type I， the followings were obtained． There were some sexual differences in the items of the life style corelated with the mental health status remarkably． In male， the great factors related to the GHQ score were “Harvard－Step－Test” that indicated the general physical endurance， irregularity of eating habbit”， evaluation of student life”，and ‘‘the frequency of intentional neglect of class” in order of intensity． That is to say， general phyisical endurance and living a regular life and a substantial student life were exeedingly concerned in the mental health status． In female，“evaluation of student life”in itself like“the school record”，was most important． And also greatly participated “anxiety about health” like sickly，“increase and decrease in body weight” and “gamble”“Irregularity of eating habbit”“hours of spare time”and “average hours of sleep”also contributed to the GHQ score

Display of both N- and C-terminal target fusion proteins on the Aspergillus oryzae cell surface using a chitin-binding module

A novel cell surface display system in Aspergillus oryzae was established by using a chitin-binding module (CBM) from Saccharomyces cerevisiae as an anchor protein. CBM was fused to the N or C terminus of green fluorescent protein (GFP) and the fusion proteins (GFP-CBM and CBM-GFP) were expressed using A. oryzae as a host. Western blotting and fluorescence microscopy analysis showed that both GFP-CBM and CBM-GFP were successfully expressed on the cell surface. In addition, cell surface display of triacylglycerol lipase from A. oryzae (tglA), while retaining its activity, was also successfully demonstrated using CBM as an anchor protein. The activity of tglA was significantly higher when tglA was fused to the C terminus than N terminus of CBM. Together, these results show that CBM used as a first anchor protein enables the fusion of both the N and/or C terminus of a target protein

ChIP-Seq Data Mining: Remarkable Differences in NRSF/REST Target Genes between Human ESC and ESC-Derived Neurons

The neuron-restrictive silencer factor (NRSF) is a zinc finger transcription factor that represses neuronal gene transcription in non-neuronal cells by binding to the consensus repressor element-1 (RE1) located in regulatory regions of target genes. NRSF silences the expression of a wide range of target genes involved in neuron-specific functions. Previous studies showed that aberrant regulation of NRSF plays a key role in the pathological process of human neurodegenerative diseases. However, a comprehensive set of NRSF target genes relevant to human neuronal functions has not yet been characterized. We performed genome-wide data mining from chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) datasets of NRSF binding sites in human embryonic stem cells (ESC) and the corresponding ESC-derived neurons, retrieved from the database of the ENCODE/HAIB project. Using bioinformatics tools such as Avadis NGS and MACS, we identified 2,172 NRSF target genes in ESC and 308 genes in ESC-derived neurons based on stringent criteria. Only 40 NRSF target genes overlapped between both data sets. According to motif analysis, binding regions showed an enrichment of the consensus RE1 sites in ESC, whereas they were mainly located in poorly defined non-RE1 sites in ESC-derived neurons. Molecular pathways of NRSF target genes were linked with various neuronal functions in ESC, such as neuroactive ligand-receptor interaction, CREB signaling, and axonal guidance signaling, while they were not directed to neuron-specific functions in ESC-derived neurons. Remarkable differences in ChIP-Seq-based NRSF target genes and pathways between ESC and ESC-derived neurons suggested that NRSF-mediated silencing of target genes is highly effective in human ESC but not in ESC-derived neurons

Pathway Analysis of ChIP-Seq-Based NRF1 Target Genes Suggests a Logical Hypothesis of their Involvement in the Pathogenesis of Neurodegenerative Diseases

This is an open access article published under the Creative Commons CC-BY-NC 3.0 license

RNA-Seq Data Mining: Downregulation of NeuroD6 Serves as a Possible Biomarker for Alzheimer’s Disease Brains

Alzheimer’s disease (AD) is the most common cause of dementia worldwide with no curative therapies currently available. Previously, global transcriptome analysis of AD brains by microarray failed to identify the set of consistently deregulated genes for biomarker development of AD. Therefore, the molecular pathogenesis of AD remains largely unknown. Whole RNA sequencing (RNA-Seq) is an innovative technology for the comprehensive transcriptome profiling on a genome-wide scale that overcomes several drawbacks of the microarray-based approach. To identify biomarker genes for AD, we analyzed a RNA-Seq dataset composed of the comprehensive transcriptome of autopsized AD brains derived from two independent cohorts. We identified the core set of 522 genes deregulated in AD brains shared between both, compared with normal control subjects. They included downregulation of neuronal differentiation 6 (NeuroD6), a basic helix-loop-helix (bHLH) transcription factor involved in neuronal development, differentiation, and survival in AD brains of both cohorts. We verified the results of RNA-Seq by analyzing three microarray datasets of AD brains different in brain regions, ethnicities, and microarray platforms. Thus, both RNA-Seq and microarray data analysis indicated consistent downregulation of NeuroD6 in AD brains. These results suggested that downregulation of NeuroD6 serves as a possible biomarker for AD brains

Development of a quality of work life scale for Japanese community pharmacists: a questionnaire survey mostly in large companies

Abstract Background Human resource management may become complex for community pharmacists owing to recent changes in work volume and content. Few studies have examined job satisfaction, well-being, and quality of work life (QWL) among community pharmacists in Japan. This study focused on QWL, a more comprehensive concept than job satisfaction, and aimed to develop the QWL questionnaire for Japanese community pharmacists (the QWLQ for JCP) and assess its reliability and validity. Methods A questionnaire survey was conducted among 2027 pharmacists who worked in pharmacies with the cooperation of 20 corporations running pharmacies. Collected data were subjected to principal component factor analysis with Promax rotation via SPSS Windows version 28. Results The factor analysis used data from 1966 pharmacists. In total, five significant components, which formed the basis of the QWLQ for JCP, were identified. These included “Influence of work on mind and body,” “Relationships with colleagues,” “Relationship with the boss,” “Meaning of existence in the workplace,” and “Pride in work.” Cronbach’s alpha, which expressed reliability, ranged from 0.585 to 0.854 for all the subscales. Conclusion The QWLQ for the JCP significantly explained the concept of QWL, which indicated that its validity was sufficient