Estimating the Functional Consequences of Intra-Specific Trichome Variation of Tomato, \u3ci\u3eSolanum lycopersicum\u3c/i\u3e on the Specialist Herbivore, \u3ci\u3eManduca sexta\u3c/i\u3e

Chapter 1: Plants have a wide array of defenses against herbivores and hence they must confer trade-offs between growth and defense related traits. By using Solanum as a model genus, the major plant defense fitness trade-off hypotheses are reviewed in this chapter along with their assumptions and examples. Chapter 2: This chapter provides an overview of the enormous intra-specific diversity in trichome morphology in tomato, Solanum lycopersicum. Also, the trichome sub-types are analyzed for the variations in density and dimensions in 10 varieties of tomato by using desktop scanning electron microscope. Chapter 3: This chapter examines the functional consequences of intra-specific diversity of trichome traits in tomato on the feeding, growth, and development of Solanaceae specialist herbivore, Manduca sexta. Chapter 4: This chapter compares the trichome efficiency of three different methods used to extract trichomes from plant leaf surfaces and provides pros and cons for each of them using 5 plant species that vary in their trichomes. Chapter 5: This chapter provides the overview of major findings of my research and suggests the future directions in the field of interactions between plant physical defenses and the insect herbivores

Thin Film Nanofibrous Composite Electrospun Membrane for Separation of Salts

Ph.DDOCTOR OF PHILOSOPH

Surface modification of electrospun poly(vinylidene fluoride) nanofibrous microfiltration membrane

Master'sMASTER OF ENGINEERIN

Diabetes mellitus and odontogenic infections: a life threatening combination in Ludwig's angina

Background: The present study was a prospective study which was aimed to assess the risk factors, microbiological profile, management strategies of Ludwig’s angina patients and its association with odontogenic infections and diabetes mellitus. Methods: The study population consisted of 40 patients of Ludwig’s angina those presented to and were managed in the department of ENT, Government Medical College, Amritsar, Punjab, India, from January 2022 to May 2023. All the patients exhibiting clinical symptoms and signs of Ludwig’s angina were examined and a detailed history of the duration of each symptom, present and past illness, dental infection, throat infection, diabetes mellitus was taken. Results: It was observed that in 85% cases of Ludwig’s angina history of dental infection was present. History of diabetes mellitus was present in 50% cases followed by tooth extraction (10%). Streptococcus viridans was found in 22.5% patients, Staphylococcus aureus (12.5%) and no growth was seen in 52.5% cases. Major co-morbidity was diabetes mellitus (50%), followed by HIV and HCV infections in 15% patients. Surgical drainage was performed in 90% cases including tracheostomy in 12.5% cases rest 10% patients were treated medically. Conclusions: Uncontrolled diabetes mellitus, delayed treatment of odontogenic infection complicates the management of Ludwig’s angina and leads to life threatening complications. Ludwig’s angina should be actively treated as a surgical and medical emergency in which incision and drainage is required as early as possible. Tracheostomy should be considered as an emergency measure to relieve the respiratory obstruction

Morphological characterization of intraspecific variation for trichome traits in tomato (Solanum lycopersicum)

Trichomes, the hairlike protuberances in plants, have been well known to act as the first line of defense against herbivores, and abiotic stresses, along with other structural defenses such as spines, thorns, and waxes. We previously reported the tremendous variation in trichome traits among different wild and cultivated Solanum species and demonstrated that trichomes types and density are traditionally miscalculated and often misnamed. However, intraspecific variation in trichome traits is poorly understood, although this has implications for stress tolerance and resistance breeding programs in economically important crop species and can also mediate ecological interactions at multiple trophic levels in their wild congeners. In this study, using tomato as a model, we characterized the trichomes from 10 commonly grown varieties using a minimal sample prep desktop scanning electron microscopy, and followed up with estimating their dimensions across the varieties and trichome types. We hypothesized that although trichome number may vary, the varieties will have similar trichome types, based on current literature. Our results show that there is significant variation for trichome number as well as dimensions of trichome types among these varieties. Furthermore, when we separately analyzed the number and dimensions of commonly found glandular and non-glandular trichomes, the results were consistent with broad assessment of trichomes, showing consistent variation among varieties, suggesting that trichome studies should not be limited to basic classification into glandular and non-glandular, and should accommodate the sub-types and their dimensions

Revisiting plant defense-fitness trade-off hypotheses using Solanum as a model genus

Plants possess physical and chemical defenses which have been found to deter herbivores that feed and oviposit on them. Despite having wide variety of defenses which can be constitutive or induced, plants are attacked and damaged by insects associated with different mouthparts and feeding habits. Since these defenses are costly, trade-offs for growth and defense traits play an important role in warding off the herbivores, with consequences for plant and herbivore growth, development and fitness. Solanum is a diverse and rich genus comprising of over 1,500 species with economic and ecological importance. Although a large number of studies on Solanum species with different herbivores have been carried out to understand plant defenses and herbivore counter defenses, they have primarily focused on pairwise interactions, and a few species of economic and ecological importance. Therefore, a detailed and updated understanding of the integrated defense system (sum of total defenses and trade-offs) is still lacking. Through this review, we take a closer look at the most common plant defense hypotheses, their assumptions and trade-offs and also a comprehensive evaluation of studies that use the genus Solanum as their host plant, and their generalist and specialist herbivores from different feeding guilds. Overall, review emphasizes on using ubiquitous Solanum genus and working toward building an integrated model which can predict defense-fitness-trade-offs in various systems with maximum accuracy and minimum deviations from realistic results

Revisiting plant defense-fitness trade-off hypotheses using Solanum as a model genus

Plants possess physical and chemical defenses which have been found to deter herbivores that feed and oviposit on them. Despite having wide variety of defenses which can be constitutive or induced, plants are attacked and damaged by insects associated with different mouthparts and feeding habits. Since these defenses are costly, trade-offs for growth and defense traits play an important role in warding off the herbivores, with consequences for plant and herbivore growth, development and fitness. Solanum is a diverse and rich genus comprising of over 1,500 species with economic and ecological importance. Although a large number of studies on Solanum species with different herbivores have been carried out to understand plant defenses and herbivore counter defenses, they have primarily focused on pairwise interactions, and a few species of economic and ecological importance. Therefore, a detailed and updated understanding of the integrated defense system (sum of total defenses and trade-offs) is still lacking. Through this review, we take a closer look at the most common plant defense hypotheses, their assumptions and trade-offs and also a comprehensive evaluation of studies that use the genus Solanum as their host plant, and their generalist and specialist herbivores from different feeding guilds. Overall, review emphasizes on using ubiquitous Solanum genus and working toward building an integrated model which can predict defense-fitness-trade-offs in various systems with maximum accuracy and minimum deviations from realistic results

Table_1_Revisiting plant defense-fitness trade-off hypotheses using Solanum as a model genus.docx

Plants possess physical and chemical defenses which have been found to deter herbivores that feed and oviposit on them. Despite having wide variety of defenses which can be constitutive or induced, plants are attacked and damaged by insects associated with different mouthparts and feeding habits. Since these defenses are costly, trade-offs for growth and defense traits play an important role in warding off the herbivores, with consequences for plant and herbivore growth, development and fitness. Solanum is a diverse and rich genus comprising of over 1,500 species with economic and ecological importance. Although a large number of studies on Solanum species with different herbivores have been carried out to understand plant defenses and herbivore counter defenses, they have primarily focused on pairwise interactions, and a few species of economic and ecological importance. Therefore, a detailed and updated understanding of the integrated defense system (sum of total defenses and trade-offs) is still lacking. Through this review, we take a closer look at the most common plant defense hypotheses, their assumptions and trade-offs and also a comprehensive evaluation of studies that use the genus Solanum as their host plant, and their generalist and specialist herbivores from different feeding guilds. Overall, review emphasizes on using ubiquitous Solanum genus and working toward building an integrated model which can predict defense-fitness-trade-offs in various systems with maximum accuracy and minimum deviations from realistic results.</p

Can trap color affect arthropod community attraction in agroecosystems? A test using yellow vane and colorless traps

Vane trapping is one of the most effective methods for sampling flower-visiting arthropods. Despite its importance in pollinator studies, the effects of trap color on the abundance and richness of pollinators are less understood. To test this, we conducted a 3-season field experiment over 2 years with two types of vane traps: yellow and colorless. We set up twelve traps each in three field sites within the Lower Rio Grande Valley in south Texas, planted with Vigna unguiculata, Crotalaria juncea, Raphanus raphanistrum, and Sorghum drummondii. At each site, six colorless vane and six yellow vane traps were placed equidistant from each other. The experiment was replicated three times across three seasons, first during the pre-flowering season, when the crops were in full bloom, and when there was no crop on the field. In total, we collected 1912 insects, out of which 76.7% were pollinators. Generalized Linear Regression analyses showed that yellow traps consistently attracted significantly more arthropods and pollinators, but these differences were also season dependent. Furthermore, we noticed that Hymenoptera, followed by Coleoptera, were the most prevalent orders in both the yellow vane and colorless vane traps. Interestingly, although there was no significant difference in species richness of the arthropods in the yellow and colorless vane traps, our results suggest that trap color plays a significant role in capturing pollinators, including non-target arthropods. Our data add another line of evidence suggesting that trap color should be accounted for designing experiments that estimate pollinator and arthropod community diversity