DEVELOPMENT OF SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS DETERMINATION OF ARTESUNATE AND CURCUMIN IN LIPOSOMAL FORMULATION

The present study is very expectant towards the development of co-encapsulated liposomal formulation. The assay method for artesunate has a challenge because it doesnot have a readily recognisable absorption chromophore needed for UV spectroscopy. Curcumin, a lipid soluble antioxidant, exhibits solvent and medium sensitive absorption and fluorescence properties. A simple and rapid method involving two reaction steps has been developed for assay of artesunate and curcumin combination in liposomal formulation. Stock solution of artesunate and curcumin were diluted to final concentration of 10 µg/ml. The UV scan of 10 µg/ml solution of artesunate and curcumin combinations showed the absorbtion maxima at 240 nm and 425 nm respectively. The coloured complex obeyed the Beer's law in the concentration range of 2-20 µg/ml. The above methods is rapid tool for routine analysis of artesunate and curcumin in novel carrier system. The recovery studies confirmed the accuracy and precision of the methods. Keywords: Artesunate, Curcumin, Liposomal

Formulation Development and Evaluation of Pravastatin-Loaded Nanogel for Hyperlipidemia Management

Hyperlipidemia is a crucial risk factor for the initiation and progression of atherosclerosis, ultimately leading to cardiovascular disease. The nanogel-based nanoplatform has emerged as an extremely promising drug delivery technology. Pravastatin Sodium (PS) is a cholesterol-lowering drug used to treat hyperlipidemia. This study aimed to fabricate Pravastatin-loaded nanogel for evaluation of its effect in hyperlipidemia treatment. Pravastatin-loaded chitosan nanoparticles (PS-CS-NPs) were prepared by the ionic gelation method; then, these prepared NPs were converted to nanogel by adding a specified amount of 5% poloxamer solution. Various parameters, including drug entrapment efficacy, in vitro drug release, and hemolytic activity of the developed and optimized formulation, were evaluated. The in vitro drug release of the nanogel formulation revealed the sustained release (59.63% in 24 h) of the drug. The drug excipients compatibility studies revealed no interaction between the drug and the screened excipients. Higher drug entrapment efficacy was observed. The hemolytic activity showed lesser toxicity in nanoformulation than the pure drug solution. These findings support the prospective use of orally administered pravastatin-loaded nanogel as an effective and safe nano delivery system in hyperlipidemia treatment

Formulation Development and Evaluation of Pravastatin-Loaded Nanogel for Hyperlipidemia Management.

Hyperlipidemia is a crucial risk factor for the initiation and progression of atherosclerosis, ultimately leading to cardiovascular disease. The nanogel-based nanoplatform has emerged as an extremely promising drug delivery technology. Pravastatin Sodium (PS) is a cholesterol-lowering drug used to treat hyperlipidemia. This study aimed to fabricate Pravastatin-loaded nanogel for evaluation of its effect in hyperlipidemia treatment. Pravastatin-loaded chitosan nanoparticles (PS-CS-NPs) were prepared by the ionic gelation method; then, these prepared NPs were converted to nanogel by adding a specified amount of 5% poloxamer solution. Various parameters, including drug entrapment efficacy, in vitro drug release, and hemolytic activity of the developed and optimized formulation, were evaluated. The in vitro drug release of the nanogel formulation revealed the sustained release (59.63% in 24 h) of the drug. The drug excipients compatibility studies revealed no interaction between the drug and the screened excipients. Higher drug entrapment efficacy was observed. The hemolytic activity showed lesser toxicity in nanoformulation than the pure drug solution. These findings support the prospective use of orally administered pravastatin-loaded nanogel as an effective and safe nano delivery system in hyperlipidemia treatment

Daksha: On Alert for High Energy Transients

We present Daksha, a proposed high energy transients mission for the study of electromagnetic counterparts of gravitational wave sources, and gamma ray bursts. Daksha will comprise of two satellites in low earth equatorial orbits, on opposite sides of earth. Each satellite will carry three types of detectors to cover the entire sky in an energy range from 1 keV to >1 MeV. Any transients detected on-board will be announced publicly within minutes of discovery. All photon data will be downloaded in ground station passes to obtain source positions, spectra, and light curves. In addition, Daksha will address a wide range of science cases including monitoring X-ray pulsars, studies of magnetars, solar flares, searches for fast radio burst counterparts, routine monitoring of bright persistent high energy sources, terrestrial gamma-ray flashes, and probing primordial black hole abundances through lensing. In this paper, we discuss the technical capabilities of Daksha, while the detailed science case is discussed in a separate paper.Comment: 9 pages, 3 figures, 1 table. Additional information about the mission is available at https://www.dakshasat.in

Science with the Daksha High Energy Transients Mission

We present the science case for the proposed Daksha high energy transients mission. Daksha will comprise of two satellites covering the entire sky from 1~keV to $>1$~MeV. The primary objectives of the mission are to discover and characterize electromagnetic counterparts to gravitational wave source; and to study Gamma Ray Bursts (GRBs). Daksha is a versatile all-sky monitor that can address a wide variety of science cases. With its broadband spectral response, high sensitivity, and continuous all-sky coverage, it will discover fainter and rarer sources than any other existing or proposed mission. Daksha can make key strides in GRB research with polarization studies, prompt soft spectroscopy, and fine time-resolved spectral studies. Daksha will provide continuous monitoring of X-ray pulsars. It will detect magnetar outbursts and high energy counterparts to Fast Radio Bursts. Using Earth occultation to measure source fluxes, the two satellites together will obtain daily flux measurements of bright hard X-ray sources including active galactic nuclei, X-ray binaries, and slow transients like Novae. Correlation studies between the two satellites can be used to probe primordial black holes through lensing. Daksha will have a set of detectors continuously pointing towards the Sun, providing excellent hard X-ray monitoring data. Closer to home, the high sensitivity and time resolution of Daksha can be leveraged for the characterization of Terrestrial Gamma-ray Flashes.Comment: 19 pages, 7 figures. Submitted to ApJ. More details about the mission at https://www.dakshasat.in

Prospects of measuring Gamma-ray Burst Polarisation with the Daksha mission

The proposed Daksha mission comprises of a pair of highly sensitive space telescopes for detecting and characterising high-energy transients such as electromagnetic counterparts of gravitational wave events and gamma-ray bursts (GRBs). Along with spectral and timing analysis, Daksha can also undertake polarisation studies of these transients, providing data crucial for understanding the source geometry and physical processes governing high-energy emission. Each Daksha satellite will have 340 pixelated Cadmium Zinc Telluride (CZT) detectors arranged in a quasi-hemispherical configuration without any field-of-view collimation (open detectors). These CZT detectors are good polarimeters in the energy range 100 -- 400 keV, and their ability to measure polarisation has been successfully demonstrated by the Cadmium Zinc Telluride Imager (CZTI) onboard AstroSat. Here we demonstrate the hard X-ray polarisation measurement capabilities of Daksha and estimate the polarisation measurement sensitivity (in terms of the Minimum Detectable Polarisation: MDP) using extensive simulations. We find that Daksha will have MDP of~$30\%$ for a fluence threshold of $10^{-4}$ erg cm$^2$ (in 10 -- 1000 keV). We estimate that with this sensitivity, if GRBs are highly polarised, Daksha can measure the polarisation of about five GRBs per year.Comment: Submitted to Journal of Astronomical Telescopes, Instruments, and Systems (JATIS

Daksha: On Alert for High Energy Transients

We present Daksha, a proposed high energy transients mission for the study of electromagnetic counterparts of gravitational wave sources, and gamma ray bursts. Daksha will comprise of two satellites in low earth equatorial orbits, on opposite sides of earth. Each satellite will carry three types of detectors to cover the entire sky in an energy range from 1 keV to >1 MeV. Any transients detected on-board will be announced publicly within minutes of discovery. All photon data will be downloaded in ground station passes to obtain source positions, spectra, and light curves. In addition, Daksha will address a wide range of science cases including monitoring X-ray pulsars, studies of magnetars, solar flares, searches for fast radio burst counterparts, routine monitoring of bright persistent high energy sources, terrestrial gamma-ray flashes, and probing primordial black hole abundances through lensing. In this paper, we discuss the technical capabilities of Daksha, while the detailed science case is discussed in a separate paper