Variance component and parameter estimates.

<p>Variance component and parameter estimates.</p

Correlations between methods.

<p>Correlations between RTL measurements from different DNA extraction methods (PG: Gentra Puregene kit; SC: DNeasy spin columns; SP: DNeasy 96 well plate): Cattle, method-specific calibrator (A); Cattle, Puregene calibrator (B); Cattle, no calibrator (C); Sheep, method-specific calibrator (D); Sheep, no calibrator (E). Regression lines and their 95% confidence interval are shown in blue and grey, respectively, with red lines reflecting a hypothetically perfect correspondence (slope of one, intercept of zero).</p

DNA integrity gels.

<p>(A) Illustrative DNA Integrity gels with gel scores. Example integrity gels for (B) Holstein Friesian cattle and (C) Soay sheep. Individual samples (represented by numbers in image) that were extracted with different DNA extraction protocols. (PG: Gentra Puregene kit, SC: DNeasy spin columns, SP: DNeasy 96 well plate; GS: calibrator DNA (“golden sample”).</p

Raw RTL and Cq values.

<p>RTL or Cq values by DNA extraction method and qPCR plate for cattle (A-E) and sheep (F-I). RTL calculated with method specific (MS) calibrator (A + F), Puregene (PG) calibrator (B), no calibrator (C+G). Cq values for telomere reaction (D+H) and control gene <i>B2M</i> (E+I). Colours represent DNA extraction methods. White: Gentra Puregene, blue: DNeasy spin columns, orange: DNeasy 96 well plate.</p

Watson et al - Data

Data used in analyses presented in this paper. Please contact the corresponding author if you wish to use this data for anything other than exact replication of our our analyses. Note that individual sheep IDs have been scrambled and will not correspond to IDs in data associated with other publications using the same study system