9 research outputs found

    Study of the interaction mechanism between hydrophilic thiol capped gold nanoparticles and melamine in aqueous medium

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    In the last years, intense efforts have been made in order to obtain colloidal-based systems capable of pointing out the presence of melamine in food samples. In this work, we reported about the recognition of melamine in aqueous solution, using gold nanoparticles stabilized with 3-mercapto-1-propanesulfonate (AuNPs-3MPS), with the aim of deepening how the recognition process works. AuNPs were synthesized using a wet chemical reduction method. The synthesized AuNPs-3MPS probe was fully characterized, before and after the recognition process, by both physicochemical (UV–vis, FT-IR, 1H-NMR, DLS and ζ-potential) and morphostructural techniques (AFM, HR-TEM). The chemical and electronic structure was also investigated by SR-XPS. The sensing method is based on the melamine-induced aggregation of AuNPs; the presence of melamine was successfully detected in the range of 2.5−500 ppm. The results achieved also demonstrate that negatively charged AuNPs-3MPS are potentially useful for determining melamine contents in aqueous solution. SR-XPS measurements allowed to understand interaction mechanism between the probe and the analyte. The presence of sulfonate groups allows a mutual interaction mediated by electrostatic bonds between nanoparticles surface thiols and positively charged amino groups of melamine molecules

    Guida ai prodotti tipici del Casentino. Itinerari fra cultura e tradizioni locali

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    Risultati finali Prin 2005. E' in discussione una traduzione in inglese da parte del Consorzio "Casentino Turismo"

    Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials

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    This paper presents the synthesis of silver nanoparticles (AgNPs) functionalized with fluorescent molecules, in particular with xanthene-based dyes, i.e., fluorescein isothiocyanate (FITC, λmax = 485 nm) and rhodamine B isothiocyanate (RITC, λmax = 555 nm). An in-depth characterization of the particle–dye systems, i.e., AgNPs–RITC and AgNPs–FITC, is presented to evaluate their chemical structure and optical properties due to the interaction between their plasmonic and absorption properties. UV–Vis spectroscopy and the dynamic light scattering (DLS) measurements confirmed the nanosize of the AgNPs–RITC and AgNPs–FITC. Synchrotron radiation X-ray photoelectron spectroscopy (SR-XPS) was used to study the chemical surface functionalization by structural characterization, confirming/examining the isothiocyanate–metal interaction. For AgNPs–RITC, in which the plasmonic and fluorescence peak are not superimposed, the transient dynamics of the dye fluorescence were also studied. Transient absorption measurements showed that by exciting the AgNPs–RITC sample at a wavelength corresponding to the AgNP plasmon resonance, it was possible to preferentially excite the RITC dye molecules attached to the surface of the NPs with respect to the free dye molecules in the solution. These results demonstrate how, by combining plasmonics and fluorescence, these AgNPs can be used as promising systems in biosensing and imaging applications

    BIOBLITZ 2013-2014 OASI DI SAN FELICE

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    The growing interest in emerging environmental crisis has increased the level of public attention and the willingness to take part in participatory science projects, commonly defined with the term \u201cCitizen Science\u201d. This phenomenon can be recorded globally in many national contexts, with a prevalence in anglophone and more industrialized countries. In the biodiversity sector, the growing demand for public involvement has been declined in many different ways, with solutions aimed at providing cognitive and participatory tools. Among these, one of the best known is the BioBlitz: a 24-hour event held in a specific place with the aim of listing as many living species as possible. This work presents the data collected during the first two BioBlitzes organized by the Maremma Natural History Museum in 2013 and 2014. Both were made in the area of the \u200b\u200b San Felice Oasis, near the terminal part of the San Leopoldo ditch. During the BioBlitz, data collected attested the presence of 627 different taxonomic entities, of which 527 were identified at the level of species and 12 at the level of subspecies. The rest were identified at higher taxonomic levels. Eleven alien species, one endemic species, 33 species protected by national and international laws and / or directives and 13 species at risk of extinction were identified
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