Environmental and Economic Analysis of an Anaerobic Co-Digestion Power Plant Integrated with a Compost Plant

Italian power generation through anaerobic digestion (AD) has grown significantly between 2009 and 2016, becoming an important renewable energy resource for the country, also thanks to the generous incentives for produced electricity available in the last years. This work focuses on the economic and environmental issues of AD technology and proposes a technoeconomic analysis of investment profitability without government support. In particular, the analysis focuses on an AD power plant fed by zootechnical wastewater and agro-industrial residues coupled to a cogeneration (CHP) system and a digestate-composting plant that produces soil fertilizers. We aim to determine the economic profitability of such AD power plants fed by innerfarm biomass wastes, exploiting digestate as fertilizer, using the cogenerated heat and taking into account the externalities (environmental benefits). Environmental analysis was carried out via a life cycle analysis (LCA), and encompassing the production of biogas, heat/electricity and compost in the downstream process. The un-released environmental emissions were converted into economic benefits by means of a stepwise approach. The results indicate that integrating a compost plant with a biogas plant can significantly increase the carbon credits of the process. The results were evaluated by means of a sensitivity analysis, and they report an IRR in the range of 6%–9% according to the Italian legislative support mechanisms, and possibilities to increase revenues with the use of digestate as fertilizer. The results significantly improve when externalities are included

Rafoxanide sensitizes colorectal cancer cells to TRAIL-mediated apoptosis

Colorectal cancer (CRC) remains a leading causes of cancer-related death in the world, mainly due to the lack of effective treatment of advanced disease. TNF-related apoptosis-inducing ligand (TRAIL)-driven cell death, a crucial event in the control of tumor growth, selectively targets malignant rather than non-transformed cells. However, the fact that cancer cells, including CRC cells, are either intrinsically resistant or acquire resistance to TRAIL, represents a major hurdle to the use of TRAIL-based strategies in the clinic. Agents able to overcome CRC cell resistance to TRAIL have thus great therapeutic potential and many researchers are making efforts to identify TRAIL sensitizers. The anthelmintic drug rafoxanide has recently emerged as a potent anti-tumor molecule for different cancer types and we recently reported that rafoxanide restrained the proliferation of CRC cells, but not of normal colonic epithelial cells, both in vitro and in a preclinical model mimicking sporadic CRC. As these findings were linked with the induction of endoplasmic reticulum stress, a phenomenon involved in the regulation of various components of the TRAIL-driven apoptotic pathway, we sought to determine whether rafoxanide could restore the sensitivity of CRC cells to TRAIL. Our data show that rafoxanide acts as a selective TRAIL sensitizer in vitro and in a syngeneic experimental model of CRC, by decreasing the levels of c-FLIP and survivin, two key molecules conferring TRAIL resistance. Collectively, our data suggest that rafoxanide could potentially be deployed as an anti-cancer drug in the combinatorial approaches aimed at overcoming CRC cell resistance to TRAIL-based therapies

Dipotassium Glycyrrhizate Improves Intestinal Mucosal Healing by Modulating Extracellular Matrix Remodeling Genes and Restoring Epithelial Barrier Functions

Gut mucosal healing (MH) is considered a key therapeutic target and prognostic parameter in the management of inflammatory bowel disease (IBD). The dipotassium glycyrrhizate (DPG), a salt of the glycoconjugated triterpene glycyrrhizin, has been shown to inhibit the High Mobility Group Box 1 (HMGB1) protein, an allarmin strongly implicated in the pathogenesis of most inflammatory and auto-immune disorders. Here we discuss new insights on how DPG acts on MH comparing the acute phase and the recovery phase from experimental colitis in mice. We found that DPG strongly accelerates MH by differently regulating pro-inflammatory (CXCL1, CXCL3, CXCL5, PTGS2, IL-1β, IL-6, CCL12, CCL7) and wound healing (COL3A1, MMP9, VTN, PLAUR, SERPINE, CSF3, FGF2, FGF7, PLAT, TIMP1) genes as observed only during the recovery phase of colitis. Relevant issue is the identification of extracellular matrix (ECM) remodeling genes, VTN, and PLAUR, as crucial genes to achieve MH during DPG treatment. Furthermore, a noticeable recovery of intestinal epithelial barrier structural organization, wound repair ability, and functionality is observed in two human colorectal adenocarcinoma cell lines exposed to DPG during inflammation. Thus, our study identifies DPG as a potent tool for controlling intestinal inflammation and improving MH

Whole exome sequencing highlights rare variants in CTCF, DNMT1, DNMT3A, EZH2 and SUV39H1 as associated with FSHD

Introduction: Despite the progress made in the study of Facioscapulohumeral Dystrophy (FSHD), the wide heterogeneity of disease complicates its diagnosis and the genotype-phenotype correlation among patients and within families. In this context, the present work employed Whole Exome Sequencing (WES) to investigate known and unknown genetic contributors that may be involved in FSHD and may represent potential disease modifiers, even in presence of a D4Z4 Reduced Allele (DRA).Methods: A cohort of 126 patients with clinical signs of FSHD were included in the study, which were characterized by D4Z4 sizing, methylation analysis and WES. Specific protocols were employed for D4Z4 sizing and methylation analysis, whereas the Illumina® Next-Seq 550 system was utilized for WES. The study included both patients with a DRA compatible with FSHD diagnosis and patients with longer D4Z4 alleles. In case of patients harboring relevant variants from WES, the molecular analysis was extended to the family members.Results: The WES data analysis highlighted 20 relevant variants, among which 14 were located in known genetic modifiers (SMCHD1, DNMT3B and LRIF1) and 6 in candidate genes (CTCF, DNMT1, DNMT3A, EZH2 and SUV39H1). Most of them were found together with a permissive short (4–7 RU) or borderline/long DRA (8–20 RU), supporting the possibility that different genes can contribute to disease heterogeneity in presence of a FSHD permissive background. The segregation and methylation analysis among family members, together with clinical findings, provided a more comprehensive picture of patients.Discussion: Our results support FSHD pathomechanism being complex with a multigenic contribution by several known (SMCHD1, DNMT3B, LRIF1) and possibly other candidate genes (CTCF, DNMT1, DNMT3A, EZH2, SUV39H1) to disease penetrance and expressivity. Our results further emphasize the importance of extending the analysis of molecular findings within the proband’s family, with the purpose of providing a broader framework for understanding single cases and allowing finer genotype-phenotype correlations in FSHD-affected families

Comparative Environmental Assessment of the Iron Fertilisers’ Production: Fe-Biochelate versus Fe-EDDHA

In response to tackling the environmental consequences of fertiliser production, biofertilisers from organic sources are strongly promoted in line with circular economy and maximising resource use. Despite the outstanding potential of bio-based fertilisers for the sustainable development of the agricultural sector, an environmental investigation of these fertilisers is required to replace synthesised fertilisers. Considering the importance of iron as a plant micronutrient and the scientific gap in the environmental assessment of relevant fertilisers, iron-based fertilisers produced in EU and US geographical zones are selected as a case study in this paper. Therefore, this study examines the environmental performance of two iron-based fertilisers (Fe-biochelate and Fe-EDDHA) by the life cycle assessment (LCA) methodology. The LCA model has been implemented in Simapro software by the ecoinvent database and ReCipe 2016 method considering 1 kg iron content as a functional unit. The results revealed that the Fe-biochelate reduced impacts (69–82%) on all relevant categories, including global warming (69%), terrestrial ecotoxicity (82%), and fossil resource scarcity (77%) in comparison with Fe-EDDHA. Soymeal and acetic acid were the main stressors identified in Fe-biochelate production, while phenol, ethylenediamine and glyoxal were the most significant contributors to the impact categories related to Fe-EDDHA. As a result, Fe-biochelate can be considered a more eco-friendly alternative to Fe-EDDHA

Life Cycle Assessment and Environmental Valuation of Biochar Production: Two Case Studies in Belgium

It is unclear whether the production of biochar is economically feasible. As a consequence, firms do not often invest in biochar production plants. However, biochar production and application might be desirable from a societal perspective as it might entail net environmental benefits. Hence, the aim of this work has been to assess and monetize the environmental impacts of biochar production systems so that the environmental aspects can be integrated with the economic and social ones later on to quantify the total return for society. Therefore, a life cycle analysis (LCA) has been performed for two potential biochar production systems in Belgium based on two different feedstocks: (i) willow and (ii) pig manure. First, the environmental impacts of the two biochar production systems are assessed from a life cycle perspective, assuming one ton of biochar as the functional unit. Therefore, LCA using SimaPro software has been performed both on the midpoint and endpoint level. Biochar production from willow achieves better results compared to biochar from pig manure for all environmental impact categories considered. In a second step, monetary valuation has been applied to the LCA results in order to weigh environmental benefits against environmental costs using the Ecotax, Ecovalue, and Stepwise approach. Consequently, sensitivity analysis investigates the impact of variation in NPK savings and byproducts of the biochar production process on monetized life cycle assessment results. As a result, it is suggested that biochar production from willow is preferred to biochar production from pig manure from an environmental point of view. In future research, those monetized environmental impacts will be integrated within existing techno-economic models that calculate the financial viability from an investor’s point of view, so that the total return for society can be quantified and the preferred biochar production system from a societal point of view can be identified

Techno-Economic Modeling of Biomass Pellet Routes: Feasibility in Italy

Wood and agricultural biomass pellets boost the potential as bio-fuels toward power production in tertiary and residential sectors. The production of pellets, however, is a multi-stage process where the supply-processing phases and the overall energy input strongly depend on the characteristics of the input biomass. In this paper, we describe the key features of the market for pellets in Italy, including national production and consumption data, production costs and prices, the available energy conversion systems, and the current regulatory issues. Moreover, we outline the main technical, economic, and end-user barriers that should be addressed in order to foster the growth of Italian pellet production. Additionally, we propose a methodology to evaluate the profitability of the pellet production chain, by assessing the investment and operation costs as a function of the quality of the raw biomass. The approach is applied to a real case study of a small firm producing wooden frames along with dry wood chips as the main by-product, which can be utilized subsequently for pellet production. Moreover, in order to optimize the size of the pellet production plant, further biomass was purchased from the market, including wood pruning and agricultural residues, wood chips from forestry, and uncontaminated residues of wood processing firms. A sensitivity analysis of the main technical and economic parameters (including the cost and quality of raw material, pellet market value, investment and operational costs, and plant lifetime) indicated that the biomass market price considerably affects the profitability of pellet production plants, particularly where the biomass has a high moisture content. Therefore, a 20% increase in the price of biomass with a high moisture content leads to a 60% fall in profitability index, turning it into negative one. This is due in particular to the costs of pre-treatment and drying of biomass, as well as to the lower energy content of wet biomass. As a result, the use of forestry residues with high moisture and high ash content, high costs of collection/transport, and high costs of pre-treatment and drying is not financially competitive

FUS Alters circRNA Metabolism in Human Motor Neurons Carrying the ALS-Linked P525L Mutation

Deregulation of RNA metabolism has emerged as one of the key events leading to the degeneration of motor neurons (MNs) in Amyotrophic Lateral Sclerosis (ALS) disease. Indeed, mutations on RNA-binding proteins (RBPs) or on proteins involved in aspects of RNA metabolism account for the majority of familiar forms of ALS. In particular, the impact of the ALS-linked mutations of the RBP FUS on many aspects of RNA-related processes has been vastly investigated. FUS plays a pivotal role in splicing regulation and its mutations severely alter the exon composition of transcripts coding for proteins involved in neurogenesis, axon guidance, and synaptic activity. In this study, by using in vitro-derived human MNs, we investigate the effect of the P525L FUS mutation on non-canonical splicing events that leads to the formation of circular RNAs (circRNAs). We observed altered levels of circRNAs in FUSP525L MNs and a preferential binding of the mutant protein to introns flanking downregulated circRNAs and containing inverted Alu repeats. For a subset of circRNAs, FUSP525L also impacts their nuclear/cytoplasmic partitioning, confirming its involvement in different processes of RNA metabolism. Finally, we assess the potential of cytoplasmic circRNAs to act as miRNA sponges, with possible implications in ALS pathogenesis