15 research outputs found
Application of the Pup1-K46 Marker to Evaluate Phosphorus-Deficient Tolerance in Lowland Rice Cultivars from Southern Thailand
Get PDFABSTRACT Phosphorus (P) deficiency severely limits rice production, which threats to global food security. Selecting rice cultivars tolerant to P deficiency is a sustainable approach for this issue. In rice, a gene-based marker Phosphate uptake 1 (Pup1-K46) has been used to evaluate P-deficient tolerance. Indisputably, the Pup1-K46 region is highly conserved in an upland rice ecotype. Its existence reflects to the tolerance of P deficiency. Unfortunately, the influence of Pup1-K46 and its application in lowland rice cultivars remains neglect. In this study, we investigated the Pup1-K46 locus among 61 lowland rice cultivars. Subsequently, several lowland rice cultivars with and without the Pup1 locus were grown under the 0.5x low P (0.25 mg/l) and high P (5 mg/l) Yoshida solution for three weeks to examine the P-deficient tolerance of rice seedlings based on the existence of Pup1-K46. The results showed that the low P solution reduced rice biomass up to 20 percent reduction with diverse degrees, depending on the rice cultivars. It markedly lowered the total P concentration but raised P use efficiency (PUE) in the shoot and root tissues. The reduction of shoot growth due to the low P availability in the Pup1 positive group was significantly lower than the Pup1 negative group. Additionally, the higher shoot PUE in the Pup1 positive group confirmed their growth performance against P deficiency. These suggest that the Pup1 locus contributes to P-deficient tolerance in lowland rice cultivars.Keywords: Pup1-K46, lowland rice, Phosphorus-deficient toleranc
Probing the Catalytic Roles of Arg548 and Gln552 in the Carboxyl Transferase Domain of the \u3cem\u3eRhizobium etli\u3c/em\u3e Pyruvate Carboxylase by Site-directed Mutagenesis
Get PDFThe roles of Arg548 and Gln552 residues in the active site of the carboxyl transferase domain of Rhizobium etli pyruvate carboxylase were investigated using site-directed mutagenesis. Mutation of Arg548 to alanine or glutamine resulted in the destabilization of the quaternary structure of the enzyme, suggesting that this residue has a structural role. Mutations R548K, Q552N, and Q552A resulted in a loss of the ability to catalyze pyruvate carboxylation, biotin-dependent decarboxylation of oxaloacetate, and the exchange of protons between pyruvate and water. These mutants retained the ability to catalyze reactions that occur at the active site of the biotin carboxylase domain, i.e., bicarbonate-dependent ATP cleavage and ADP phosphorylation by carbamoyl phosphate. The effects of oxamate on the catalysis in the biotin carboxylase domain by the R548K and Q552N mutants were similar to those on the catalysis of reactions by the wild-type enzyme. However, the presence of oxamate had no effect on the reactions catalyzed by the Q552A mutant. We propose that Arg548 and Gln552 facilitate the binding of pyruvate and the subsequent transfer of protons between pyruvate and biotin in the partial reaction catalyzed in the active site of the carboxyl transferase domain of Rhizobium etli pyruvate carboxylase
Synchronizing Nitrogen Fertilization and Planting Date to Improve Resource Use Efficiency, Productivity, and Profitability of Upland Rice
Get PDFSynchronizing nitrogen (N) fertilization with planting date (PD) could enhance resource use efficiency and profitability of upland rice (Oryza sativa L.) production in Thailand. The objective of the study was to assess upland rice responses to four N fertilization rates (NFRs) and three planting dates. Field experiments were conducted during two growing seasons under four NFRs, no N applied (N0), 30 (N30), 60 (N60), and 90 kg N ha−1 (N90), and NFR were applied at the initiation of tillering and panicle emergence stages. The planting dates selected were early (PD1), intermedium (PD2), and late planting (PD3) between September and December of each season. The NFRs and planting dates had a significant influence on N uptake, N use efficiency (NUE), crop water productivity, yield and yield attributes, and profitability of upland rice production. A linear relationship among NFRs, agronomic traits of upland rice, N uptake, and crop water productivity was observed, and a significant seasonal effect was indicated. Fertilization at N90 under PD2 enhanced yields, yield attributes, and grain yields, as well as crop water productivity by 56 and 105% during the second and first seasons, respectively. Grain N, total N, and straw N were increased by 159, 159, and 160%, and by 90, 114, and 153%, during the first and second seasons, respectively. Enhanced N efficiencies, including agronomic efficiency, recovery efficiency, partial factor productivity, and N harvest index, at varying NFRs were observed under PD2 during both seasons. Highly significant (p < 0.001) and positive associations were observed among agronomic attributes, N uptake, NUE, and crop water productivity of upland rice in correlation assessment. Profitability from grain yields was observed with N fertilization and N90 resulted in maximum profit under all the PDs. However, the highest marginal benefit-cost ratio was observed at N60 under PD2 during both seasons. The results suggest that the NFR of 90 kg N ha−1 and planting at the end of September or start of October would enhance resource use efficiency and productivity, and maximize profitability. Furthermore, long–term field investigations with a range of NFRs and adopting forecasting measures to adjust the planting date for upland rice are recommended
Beneficial Effects of Silicon Fertilizer on Growth and Physiological Responses in Oil Palm
No full textVigorous and well-established nursery seedlings are an important component of sustainable oil palm production. We postulated that Si fertilization at the seedling stage could help to achieve improved performance of oil palm seedlings leading to healthy and vigorous nursery establishment. In this study, we evaluated the growth and physiological responses of oil palm Tenera hybrid seedlings under three Si fertilization treatments and a control including (i) 0 g Ca2SiO4 (T0), (ii) 0.5 g Ca2SiO4 (T1), (iii) 3.5 g Ca2SiO4 (T2), and (iv) 7.0 g Ca2SiO4 (T3) per plant per month. Ca2SiO4 was used as the Si fertilizer source and was applied for four consecutive months. Nondestructive data including stem diameter, plant height, leaf length, photosynthetic rate, leaf angle, and leaf thickness and destructive data including leaf, stem, and root fresh weight and dry weight, as well as chlorophyll a, Si, and nitrogen contents, were recorded before treatment (0 DAT), as well as 60 (60 DAT) and 120 days after treatment (120 DAT). Results indicated that Si fertilization enhanced Si accumulation in oil palm seedlings, and maximum accumulation was observed in the aerial parts especially the leaves with the highest accumulation of 0.89 % dry weight at T3. Higher Si accumulation stimulated the growth of seedlings; a total fresh weight of 834.28 g and a total dry weight of 194.34 g were observed at T3. Chlorophyll a content (0.83 gm−2) and net photosynthetic rate (4.98 µM CO2·m−2·s−1) were also observed at T3. Leaf morphology was not significantly influenced under Si fertilization, whereas the nitrogen content of seedlings was significantly increased. Correlation analysis revealed a highly significant and positive association among Si accumulation, chlorophyll a content, photosynthetic rate, total fresh weight, total dry weight, and nitrogen content of seedlings, indicating that Si fertilization enhanced the performance of these attributes. On the basis of the research evidence, it was concluded that Si fertilization should be considered for improved nutrient management for oil palm seedling and nursery production
Impact of Nitrogen Application Rates on Upland Rice Performance, Planted under Varying Sowing Times
No full textApplication of suitable nitrogen (N) fertilizer application rate (NR) with respect to sowing time (ST) could help to maximize the performance and productivity of upland rice in Southern Thailand. The 2-year experiments were conducted in the sheds to evaluate the agronomic responses of the upland rice genotype, Dawk Pa–yawm, under various combinations of NR and ST between 2018–2019 and 2019–2020 aimed at obtaining sufficient research evidence for the improved design of long-term field trials in Southern Thailand. As with the initial research, four NR were applied as N0 with no applied N, 1.6 g N pot−1, 3.2 g N pot−1 and 4.8 g N pot−1, and experiments were grown under three ST including early (ST1), medium (ST2) and late sowing (ST3). Results from the experiments indicate that the application of 4.8 g N pot−1 resulted in maximum grain yield under all ST in both years. However, a maximum increase in grain yield was observed under ST2 by 54–101% in 2018–2019 and by 276–339% in 2019–2020. Maximum grain N uptake of 0.57 and 0.82 g pot−1 was also observed at NR 4.8 g N pot−1 under ST2 in both years, respectively. Application of NR 4.8 g N pot−1 resulted in the highest N agronomic efficiency (NAE), nitrogen use efficiency (NUE) and water use efficiency (WUE). However, the performance of yield and yield attributes, N uptake, N use efficiencies and WUE were declined in late sowing (ST3). Significant positive association among yield, yield attributes, N uptake and WUE indicated that an increase in NR up to 4.8 g N pot−1 improved the performance of Dawk Pa–yawm. The results suggest that the application of 4.8 g N pot−1 (90 kg N ha−1) for upland rice being grown during September (ST2) would enhance N use efficiencies, WUE and ultimately improve the yield of upland rice. However, field investigations for current study should be considered prior to general recommendations. Moreover, based on the findings of this study, the importance of variable climatic conditions in the field, and the variability in genotypic response to utilize available N and soil moisture, authors suggest considering more levels of NR and intervals for ST with a greater number of upland rice genotypes to observe variations in field experiments for the precise optimization of NR according to ST
Beneficial Effects of Silicon Fertilizer on Growth and Physiological Responses in Oil Palm
No full textVigorous and well-established nursery seedlings are an important component of sustainable oil palm production. We postulated that Si fertilization at the seedling stage could help to achieve improved performance of oil palm seedlings leading to healthy and vigorous nursery establishment. In this study, we evaluated the growth and physiological responses of oil palm Tenera hybrid seedlings under three Si fertilization treatments and a control including (i) 0 g Ca2SiO4 (T0), (ii) 0.5 g Ca2SiO4 (T1), (iii) 3.5 g Ca2SiO4 (T2), and (iv) 7.0 g Ca2SiO4 (T3) per plant per month. Ca2SiO4 was used as the Si fertilizer source and was applied for four consecutive months. Nondestructive data including stem diameter, plant height, leaf length, photosynthetic rate, leaf angle, and leaf thickness and destructive data including leaf, stem, and root fresh weight and dry weight, as well as chlorophyll a, Si, and nitrogen contents, were recorded before treatment (0 DAT), as well as 60 (60 DAT) and 120 days after treatment (120 DAT). Results indicated that Si fertilization enhanced Si accumulation in oil palm seedlings, and maximum accumulation was observed in the aerial parts especially the leaves with the highest accumulation of 0.89 % dry weight at T3. Higher Si accumulation stimulated the growth of seedlings; a total fresh weight of 834.28 g and a total dry weight of 194.34 g were observed at T3. Chlorophyll a content (0.83 gm−2) and net photosynthetic rate (4.98 µM CO2·m−2·s−1) were also observed at T3. Leaf morphology was not significantly influenced under Si fertilization, whereas the nitrogen content of seedlings was significantly increased. Correlation analysis revealed a highly significant and positive association among Si accumulation, chlorophyll a content, photosynthetic rate, total fresh weight, total dry weight, and nitrogen content of seedlings, indicating that Si fertilization enhanced the performance of these attributes. On the basis of the research evidence, it was concluded that Si fertilization should be considered for improved nutrient management for oil palm seedling and nursery production
Impact of Nitrogen Application Rates on Upland Rice Performance, Planted under Varying Sowing Times
No full textApplication of suitable nitrogen (N) fertilizer application rate (NR) with respect to sowing time (ST) could help to maximize the performance and productivity of upland rice in Southern Thailand. The 2-year experiments were conducted in the sheds to evaluate the agronomic responses of the upland rice genotype, Dawk Pa–yawm, under various combinations of NR and ST between 2018–2019 and 2019–2020 aimed at obtaining sufficient research evidence for the improved design of long-term field trials in Southern Thailand. As with the initial research, four NR were applied as N0 with no applied N, 1.6 g N pot−1, 3.2 g N pot−1 and 4.8 g N pot−1, and experiments were grown under three ST including early (ST1), medium (ST2) and late sowing (ST3). Results from the experiments indicate that the application of 4.8 g N pot−1 resulted in maximum grain yield under all ST in both years. However, a maximum increase in grain yield was observed under ST2 by 54–101% in 2018–2019 and by 276–339% in 2019–2020. Maximum grain N uptake of 0.57 and 0.82 g pot−1 was also observed at NR 4.8 g N pot−1 under ST2 in both years, respectively. Application of NR 4.8 g N pot−1 resulted in the highest N agronomic efficiency (NAE), nitrogen use efficiency (NUE) and water use efficiency (WUE). However, the performance of yield and yield attributes, N uptake, N use efficiencies and WUE were declined in late sowing (ST3). Significant positive association among yield, yield attributes, N uptake and WUE indicated that an increase in NR up to 4.8 g N pot−1 improved the performance of Dawk Pa–yawm. The results suggest that the application of 4.8 g N pot−1 (90 kg N ha−1) for upland rice being grown during September (ST2) would enhance N use efficiencies, WUE and ultimately improve the yield of upland rice. However, field investigations for current study should be considered prior to general recommendations. Moreover, based on the findings of this study, the importance of variable climatic conditions in the field, and the variability in genotypic response to utilize available N and soil moisture, authors suggest considering more levels of NR and intervals for ST with a greater number of upland rice genotypes to observe variations in field experiments for the precise optimization of NR according to ST
Responses of Lowland Rice Genotypes under Terminal Water Stress and Identification of Drought Tolerance to Stabilize Rice Productivity in Southern Thailand
No full textLowland rice is an important cereal crop that plays a key role in the food security and the economy of Thailand. Terminal water stress (TWS) in rainfed lowland areas poses threats to rice productivity due to stress occurrence at terminal crop stages and extreme sensitivity of rice to TWS. A two-year study was conducted to characterize the performance of yield and yield attributes of twelve Thai lowland rice genotypes under TWS, to identify stress-tolerant genotypes using stress response indices and to identify promising stress indices which are correlated with grain yield (GY) under well-watered (WW) and TWS conditions for their use as rapid identifiers in a rice crop breeding program for enhancing drought stress tolerance. Measurements were recorded under WW and TWS conditions. Highly significant variations were observed amongst assessed genotypes for their yield productivity responses. According to stress response indices, genotypes were categorized into stress-tolerant and stress susceptible genotypes. Genotypes Hom Pathum, Sang Yod, Dum Ja and Pathum Thani-1 were found highly stress tolerant and relatively high yielding; genotypes Look Pla and Lep Nok were stress tolerant, whereas genotypes Chor Lung, Hom Nang Kaew and Hom Chan were moderately tolerant genotypes. Hence, stress-tolerant genotypes could be potentially used for cultivation under rainfed and water-limited conditions, where TWS is predicted particularly in southern Thailand to stabilize rice productivity. Stress tolerance indices, including stress tolerance index (STI), geometric mean productivity (GMP), mean productivity index (MPRO) and harmonic mean index (MHAR), indicated strong and positive associations with GY under WW and TWS; thus, these indices could be used to indicate stress tolerance in rice crop breeding program aimed at a rapid screening of lowland rice genotypes for stress tolerance
Potential Impacts of Water Stress on Rice Biomass Composition and Feedstock Availability for Bioenergy Production
No full textBioenergy from rice biomass feedstock is considered one of the potential clean energy resources and several small biomass-based powerplants have been established in rice–growing areas of Thailand. Rice production is significantly affected by drought occurrence which results in declined biomass production and quality. The impact of water stress (WS) was evaluated on six rice cultivars for biomass quality, production and bioenergy potential. Rice cultivars were experimented on in the field under well–watered (WW) and WS conditions. Data for biomass contributing parameters were collected at harvest whereas rice biomass samples were analyzed for proximate and lignocellulosic contents. Results indicated that WS negatively influenced crop performance resulting in 11–41% declined biomass yield (BY). Stability assessment indicated that cultivars Hom Pathum and Dum Ja were stress–tolerant as they exhibited smaller reductions by 11% in their BY under WS. Statistics for proximate components indicated a significant negative impact influencing biomass quality as ash contents of Hom Chan, Dum Ja and RD-15 were increased by 4–29%. Lignocellulosic analysis indicated, an increase in lignin contents of Hom Nang Kaew, Hom Pathum, Dum Ja and RD–15 ranging 7–39%. Reduced biomass production resulted in a 10–42% reduction in bioenergy potential (E). Results proved that cultivation of stress-susceptible cultivars or farmer’s choice and occurrence of WS during crop growth will reduce biomass production, biomass feedstock availability to biomass-based powerplants and affect powerplant’s conversion efficiency resulting in declined bioenergy production
