Camperdown Hemoglobin Associated With β° Thalassemia In A Brazilian Child

We report the coexistence of Hb Camperdown [β 104 (G6) Arg → Ser] and β°-thalassemia [β39 (Gln → stop codon)] in a nine-month-old Brazilian boy. He had a relatively more severe hypochromic and microcytic anemia in comparison to his mother's β-thalassemia trait. His Hb Camperdown heterozygous father was clinically and hematologically normal. To our knowledge, this is the first description of an association of β°-thalassemia with Hb Camperdown. Copyright by the Brazilian Society of Genetics.283394396Araújo, A.S., Silva, W.A., Leao, S.A., Bandeira, F.C., Petrou, M., Modell, B., Zago, M.A., A different molecular pattern of β-thalassemia mutations in Northeast of Brazil (2003) Hemoglobin, 27, pp. 211-217Amone, A., X-ray diffraction study of binding of 2,3-diphosphoglycerate to human deoxyhemoglobin (1972) Nature, 237, pp. 146-149Bertuzzo, C.S., Sonati, M.F., Costa, F.F., Hematological phenotype and the type of β thalassemia mutation in Brazil (1997) Braz J Genet, 20, pp. 319-321Bianco, I., Graziani, B., Carboni, C., Genetic patterns in thalassemia intermedia (constitutional microcytic anemia). Familial hematological and biosynthetic studies (1977) Hum Hered, 27, pp. 257-272Blouquit, Y., Lacombe, C., Arous, N., Le Qurrec, A., Branconnier, F., Bonhomme, J., Soummer, A.M., Galacteros, F., Seven new cases of hemoglobin Camperdown alpha 2 beta 2 104 (G6) ARG → SER found in Malta, Sicily and Tunisia (1984) Hemoglobin, 8, pp. 613-619Chang, J.C., Kan, Y.W., β°-thalassemia, a nonsense mutation in man (1979) Proc Natl Acad Sci USA, 76, pp. 2886-2889Clarke, G.M., Higgins, T.N., Laboratory investigation of hemoglobinopathies and thalassemias: Review and update (2000) Clin Chem, 46, pp. 1284-1290Fonseca, S.F., Kerbauy, J., Escrivçao, C., Figueiredo, M.S., Cançado, R., Arruda, V.R., Saad, S.T.O., Costa, F.F., Genetic analysis of beta-thalassemia major and beta-thalassemia intermedia in Brazil (1998) Hemoglobin, 22, pp. 197-207Grignoli, C.R.E., Carvalho, M.H., Kimura, E.M., Sonati, M.F., Arruda, V.R., Saad, S.T.O., Costa, F.F., β°-thalassemia resulting from a novel mutation: β66/u → stop codon (2000) Eur J Haematol, 64, pp. 137-138Kimura, E.M., Grignoli, C.R.E., Pinheiro, V.R.P., Costa, F.F., Sonati, M.F., Thalassemia intermedia as a result of heterozygosis for β°-thallassemia and αααanti3.7/αα genotype in a Brazilian patient (2003) Braz J Med Biol Res, 36, pp. 699-701Kister, J., Barbadjian, J., Blouquit, Y., Bohn, B., Galacteros, F., Poyart, C., Inhibition of oxygen-linked anion binding in Hb Camperdown [α2β2 104 (G6) ARG → SER] (1989) Hemoglobin, 13, pp. 567-578Miranda, S.R.P., Kimura, E.M., Teixeira, R.C., Bertuzzo, C.S., Ramalho, A.A., Saad, S.T.O., Costa, F.F., Hb Camperdown [α2β2 104 (G6) ARG → SER] identified by DNA analysis in a Brazilian family (1996) Hemoglobin, 20, pp. 147-153Old, J.M., Screening arid genetic diagnosis of haemoglobin disorders (2003) Blood Rev, 17, pp. 43-53Olivieri, N.F., The β-thalassemias (1999) N Engl J Med, 341, pp. 99-109Thein, S.L., Genetic insights into the clinical diversity of beta thalassaemia (2004) Br J Haematol, 124, pp. 264-274Weatherall, D.J., Clegg, J.B., Inherited haemoglobin disorders: An increasing global health problem (2001) Bull World Health Organ, 79, pp. 704-712Wilkinson, T., Chua, C.G., Carrell, R.W., Robin, H., Exner, T., Lee, K.M., Kronenberg, H., Haemoglobin Camperdown β 104(G6) Arginine leads to serine (1975) Biochim Biophys Acta, 393, pp. 195-200Zago, M.A., Costa, F.F., Hereditary hemoglobin disorders in Brazil (1985) Trans R Soc Trop Med Hyg, 79, pp. 385-38

COMPORTAMENTO DA INGESTÃO EM BOVINOS (RUMINANTES) EM PASTAGEM DE CAPIM Brachiaria decumbens NA REGIÃO CENTRO-OESTE DO BRASIL

An experiment has been carried out aiming to evaluate the ingestion behavior of young heifers, heifers and cows in pastures of Brachiaria decumbens, in the system of continuous stocking with three categories representing the treatments, each one with ten repetitions. The experimental period  was of 30 days, being 20 for adaptation of the experimental animals and 10 for three evaluations with duration of 24 hours each, in intervals of five days. The results of the Tukey test demonstrated that the cows grazed during more time (12 hours) than the young heifers (0.5 hours) and the heifers (10.25 hours). Statistic differences were not observed among the animals for the rumination time (7.25, 7.39 and 7.40, respectivety). The cows displayed larger bite rate (41.30) than the heifers and the young heifers. This result suggest that there was a compensatory mechanism between the times of grazing and the bite rates, so that the animals with more nutritional requirement regulated the amount of ingested forage, incrementing the grazing time.Foi conduzido um experimento com o objetivo de avaliar o comportamento na ingestão de bezerras, novilhas e vacas leiteiras em pastagens de Brachiaria decumbens, no sistema de lotação contínua com taxa de lotação variável. O delineamento experimental foi inteiramente casualizado, com três categorias animais representando os tratamentos, cada um com dez repetições. O período experimental foi de 30 dias, sendo 20 para adaptação dos animais e 10 para avaliações, que consistiram de três, com duração de 24 horas cada, em intervalos de cinco dias. O teste de Tukey demonstrou que as vacas pastejaram mais tempo (12 horas) que as novilhas (10,50 horas) e as bezerras (10,25 horas). Não foi observada diferença estatística entre os animais para o tempo de ruminação (7,25, 7,39 e 7,40, horas respectivamente). A taxa de bocados diurnos foi maior para as vacas do que para os novilhos e bezerros. Os resultados sugerem que houve um mecanismo de compensação entre os tempos de pastejo e as taxas de bocado, de modo que os animais mais exigentes pudessem regular a quantidade de forragem ingerida aumentando o tempo de pastejo