3 research outputs found
Mapping QTLs in maize testcrosses with different testers and soil acidity levels
Nas regiões tropicais os solos apresentam diferentes níveis de acidez. Assim, o estudo da herança dos caracteres de importância econômica no milho nas regiões tropicais é necessário para se delinear os programas de melhoramento para os diferentes níveis de acidez do solo. Atualmente, o estudo da arquitetura dos caracteres quantitativos tem sido realizado através do mapeamento de QTLs. Nos programas de melhoramento de milho, linhagens de populações de melhoramento são cruzadas com linhagens elites (testadores) e os testecrosses são utilizados para avaliar o potencial genético de cada linhagem para o desenvolvimento de híbridos. O objetivo deste estudo foi mapear QTLs em testecrosses avaliados sob diferentes níveis de acidez do solo. Duzentas e cinqüenta e seis plantas F2, obtidas do cruzamento das linhagens L 14-04B e L 08-05F, foram genotipadas com marcadores microssatélites para a construção de um mapa genético. As 256 plantas F2 foram autofecundadas e suas respectivas progênies F2:3 foram cruzadas com os testadores L 04-05F e L 02-03D. Os testecrosses foram avaliados em três tipos de solos: solo não ácido (SNA), solo de moderada acidez (SMA) e solo de alta acidez (SAA) em três anos agrícolas em Piracicaba, SP, em látices simples 16 x 16. Foram avaliados os caracteres: produção de grãos (PG), acamamento e quebramento de plantas (ACQ), prolificidade (PROL), alturas de planta (AP) e de espiga (AE), posição relativa de espiga (PRE), florescimento masculino (FM) e feminino (FF) e intervalo entre florescimentos (IF). O método de mapeamento por intervalo composto expandido para múltiplos ambientes foi utilizado para o mapeamento de QTLs e para detectar a interação QTL x acidez do solo. O número de QTLs mapeados diferiu de acordo com o testador utilizado; por exemplo, para PG foram mapeados 20 e 39 QTLs nos testecrosses da linhagem L 04-05F (TC1) e nos testecrosses da linhagem L 02-03D (TC2), respectivamente. Houve uma grande variação nas variâncias fenotípicas explicadas pelos QTLs; por exemplo, para PG houve uma variação de 0,01% a 5,29% e para AP houve uma variação de 0,01% a 13,54%. Foram mapeados QTLs em todos os cromossomos para a PG, ACQ e PROL; e para os outros caracteres foram mapeados QTLs na maioria dos cromossomos. A maioria dos QTLs mapeados para todos os caracteres interagiu com a acidez do solo. Por exemplo, para PG cerca de 80,00% dos QTLs mapeados apresentaram interação com a acidez do solo, enquanto que para os outros caracteres a porcentagem de QTLs que interagiu com a acidez do solo variou de 50,00% para FM a 93,03% para ACQ. O grande número de QTLs que interagiu com a acidez do solo é um sério desafio para a aplicação da seleção assistida por marcadores moleculares em programas de melhoramento de milho em regiões tropicais.In tropical maize cropping areas the soils present different levels of acidity. Thus, to study the inheritance of maize traits for these areas it is necessary to conduct experiments under different levels of soil acidity. Nowadays the architecture of the polygenic traits has been assessed by means of QTL mapping. Also, in applied breeding programs, experimental lines are crossed to elite lines (testers), and the testcrosses are used to assess their genetic potential for hybrid development. The objective of this research was to map QTLs in testcrosses evaluated under three levels of soil acidity. Two hundred and fifty six F2 plants, developed from the cross between the inbreds lines L14-04B and L08-05F, were genotyped with microsatellite markers to construct a genetic map. The 256 F2 plants were selfed and their respective F2:3 progenies were testcrossed to the testers L04-05F and L02-03D, and these testcrosses were evaluated in three types of soils: non-acid soil (NAS), moderate acitity soil (MAS) and high acidity soil (HAS) in three cropping seasons in Piracicaba, SP, in 16 x 16 simple lattices. The traits recorded were: grain yield (GY), plant lodging (PL), prolificacy (PRO), plant (PH) and ear heights (EH), ear placement (EP), days to anthesis (DA), days to silking (DS), and anthesis-silking interval (ASI). The composite interval mapping extended to multiple environment was used to map QTLs and to detect QTL x soil interaction. The number of QTLs mapped was different for each tester; for instance, for GY, 20 and 39 QTLs were mapped in the testcrosses with L04-05F and L02-03D, respectively. The range of the phenotypic variance explained by the QTLs was very large for all traits; for instance for GY the range was from 0.01% to 5.29% and for plant height it was from 0.01% to 13.54%. QTLs were mapped in all chromosomes for GY, PL, and PRO; and for the other traits QTLs were mapped in almost all chromosomes. Most of the QTLs mapped for all traits interacted significantly with soil acidity. For instance, for GY about 80.00% of the QTLs mapped interacted with soil acidity, whereas for the other traits the percentage of the QTLs that interacted with soil acidity ranged from 50.00% for DS to 93.03% to PL. The high number of the QTLs that interacted with soil acidity imposes a serious challenge for marker assisted selection in maize breeding programs for tropical regions
Genetic analysis of kernel oil content in tropical maize with design III and QTL mapping
Oil content and grain yield in maize are negatively correlated, and so far the development of high-oil high-yielding hybrids has not been accomplished. Then a fully understand of the inheritance of the kernel oil content is necessary to implement a breeding program to improve both traits simultaneously. Conventional and molecular marker analyses of the design III were carried out from a reference population developed from two tropical inbred lines divergent for kernel oil content. The results showed that additive variance was quite larger than the dominance variance, and the heritability coefficient was very high. Sixteen QTL were mapped, they were not evenly distributed along the chromosomes, and accounted for 30.91% of the genetic variance. The average level of dominance computed from both conventional and QTL analysis was partial dominance. The overall results indicated that the additive effects were more important than the dominance effects, the latter were not unidirectional and then heterosis could not be exploited in crosses. Most of the favorable alleles of the QTL were in the high-oil parental inbred, which could be transferred to other inbreds via marker-assisted backcross selection. Our results coupled with reported information indicated that the development of high-oil hybrids with acceptable yields could be accomplished by using marker-assisted selection involving oil content, grain yield and its components. Finally, to exploit the xenia effect to increase even more the oil content, these hybrids should be used in the Top Cross((TM)) procedure.Conselho Nacional de Desenvolvimento Cientifico e TecnologicoConselho Nacional de Desenvolvimento Cientifico e Tecnologico [CNPq-308499/2006-9]Department of Genetics at the Agriculture College Luiz de Queiroz/University of Sao PauloDepartment of Genetics at the Agriculture College "Luiz de Queiroz"/University of Sao Paul
Water scarcity in Brazil: part 1—regionalization of the AWARE model characterization factors
Purpose
This paper presents the regionalized water scarcity characterization factors (CFs) of the available water remaining (AWARE) model, which was found by a previous study, on the water scarcity in Brazil, to be the most indicative characterization model for the water-scarce regions in Brazil. We used the national database and hydrographic delimitations defined by the National Water Agency (Agência Nacional de Águas — ANA) to generate the regionalized AWARE BR CFs.
Methods
The CFs were regionalized by hydrographic delimitations used by ANA: (i) State Hydrographic Units (SHU) and (ii) Hydrographic Regions (HR). These AWARE BR CFs were compared with the factors originally proposed by WULCA (2018) and with the Scarcity Index used by ANA to identify the scarcest regions in the country. Finally, the AWARE and AWARE BR factors were applied to a case study of Brazilian melons, evaluating the regionalization effects on the results of water scarcity analysis.
Results and discussion
The AWARE BR CFs demonstrate most consistency with the regions recognized by ANA to have water scarcity problems, such as the semiarid region. Approximately 12% of the SHUs exhibited maximum water scarcity (CF = 100) during the entire year, while 11% presented minimum scarcity factors (CF = 0.1). The comparison of hydrologic data from ANA with those from WaterGAP indicated that water availability was overestimated in WaterGAP, while demand was underestimated in different basins. The comparison of AWARE BR CFs with ANA Scarcity Index values indicated more similarity (smaller residual error) than the comparison of AWARE BR CFs with AWARE. The case study regarding the impact of water scarcity on melons showed a significant difference between characterization factors and, consequently, in the values of impact.
Conclusions
AWARE BR factors generated with national characterization data are adapted to the different regions of Brazil, exhibiting higher sensitivity to the semiarid region. This regionalization provided a more accurate representation of the scarcity in smaller basins located in larger basins, characterized by large climate variation.info:eu-repo/semantics/publishedVersio