Stalled cerebral capillary blood flow in mouse models of essential thrombocythemia and polycythemia vera revealed by in vivo two-photon imaging

Essential thrombocythemia (ET) and polycythemia vera (PV) are myeloproliferative neoplasms (MPNs) that share the JAK2(V617F) mutation in hematopoietic stem cells, leading to excessive production of predominantly platelets in ET, and predominantly red blood cells (RBCs) in PV. The major cause of morbidity and mortality in PV and ET is thrombosis, including cerebrovascular occlusive disease. OBJECTIVES: To identify the effect of excessive blood cells on cerebral microcirculation in ET and PV. We used two-photon excited fluorescence microscopy to examine cerebral blood flow in transgenic mouse models that mimic MPNs. We found that flow was 'stalled' in an elevated fraction of brain capillaries in ET (18%), PV (27%), mixed MPN (14%) and secondary (non-MPN) erythrocytosis (27%) mice, as compared with controls (3%). The fraction of capillaries with stalled flow increased when the hematocrit value exceeded 55% in PV mice, and the majority of stalled vessels contained only stationary RBCs. In contrast, the majority of stalls in ET mice were caused by platelet aggregates. Stalls had a median persistence time of 0.5 and 1 h in ET and PV mice, respectively. Our findings shed new light on potential mechanisms of neurological problems in patients with MPNs

US EPA Design Competition for Sustainability: The Market Potential for Recycled Tire Material (semester?), IPRO 352: Tire Recycling for a Better Future IPRO 352 Midterm Report Sp05

- To create prototypes for real market applications that are successful - To create a business plan which confirms a business opportunity of using recycled tire material - To attract the attention of corporate and private investors and obtain funds to cDeliverables for IPRO 352: US EPA Design Competition for Sustainability: The Market Potential for Recycled Tire Material for the Spring 2005 semeste

Operative Techniken zum Organerhalt bei benignen Uretertumoren

- To create prototypes for real market applications that are successful - To create a business plan which confirms a business opportunity of using recycled tire material - To attract the attention of corporate and private investors and obtain funds to cDeliverables for IPRO 352: US EPA Design Competition for Sustainability: The Market Potential for Recycled Tire Material for the Spring 2005 semeste

US EPA Design Competition for Sustainability: The Market Potential for Recycled Tire Material (semester?), IPRO 352

- To create prototypes for real market applications that are successful - To create a business plan which confirms a business opportunity of using recycled tire material - To attract the attention of corporate and private investors and obtain funds to cDeliverables for IPRO 352: US EPA Design Competition for Sustainability: The Market Potential for Recycled Tire Material for the Spring 2005 semeste

US EPA Design Competition for Sustainability: The Market Potential for Recycled Tire Material (semester?), IPRO 352: Tire Recycling for a Better Future IPRO 352 Poster Sp05

- To create prototypes for real market applications that are successful - To create a business plan which confirms a business opportunity of using recycled tire material - To attract the attention of corporate and private investors and obtain funds to cDeliverables for IPRO 352: US EPA Design Competition for Sustainability: The Market Potential for Recycled Tire Material for the Spring 2005 semeste