The Psychophysiology of Intrusive Cognitions: Comparing Thought Suppression Vs Acceptance

Intrusive cognitions are a natural occurrence within our stream of consciousness, however, when they become repetitive, negative, distressing, and difficult to control, they may warrant clinical concern. Thought suppression is a common control strategy used to manage intrusive thoughts even though research suggests it may actually exacerbate the problem. Conversely, acceptance-based interventions have gained recent attention as an alternative strategy for managing distressing internal experiences. Only preliminary research has focused on the psycho- and neurophysiological bases of intrusive cognitions, and their relationship to cognitive control strategies. Evidence suggests that the anterior cingulate cortex (ACC) may be a brain region critically involved in this process. The present investigation compared the subjective, behavioral, and physiological effects of a thought suppression and acceptance strategy in a sample of university students with high or low obsessive-compulsive (OC) characteristics who were exposed to an emotion-evoking film clip. Participants were instructed either to suppress or accept any intrusive cognitions during a rest period after the film clip, while monitoring for the number of intrusions. Next, psychophysiological signals and reaction times were measured while participants performed a variant of the Stroop task. The commission of errors during a forced choice task generates an error-related negativity (ERN), which is believed to index activity in the ACC. Results showed that self-reported intrusions during the rest interval were greater for the acceptance group and the high-OC group. Correlations suggested that participants who reported more effort at suppression also indicated more distress about their thoughts, whereas those who reported more acceptance indicated less distress. During Stroop task errors, the ERN was apparent as a maximal frontal negativity, and was larger for the suppression group than the acceptance group at a frontal scalp site (Fz), but not a central scalp site (Cz). Correlations between self-reported intrusions at rest and ERN amplitude indicated that participants who reported fewer intrusions demonstrated enhanced ERNs, a marker for increased ACC activity. These findings may be interpreted as supporting the hypothesis that thought suppression is associated with increased ACC activity and greater self-reported discomfort with the intrusions

Suppress or accept? A pilot study to evaluate the effect of coping strategies on ERN amplitude among individuals with obsessive-compulsive traits

Intrusive thoughts are characteristic of psychological disorders; attempts to cope can become maladaptive perpetuating the problem (e.g., thought suppression), while others can provide long-term symptoms relief (e.g., acceptance). Although emerging research begins to explore the neural correlates of these strategies in healthy population, it is important to explore these strategies in populations more likely to naturally attempt to use such strategies (clinical symptoms). The present study explored if the use of cognitive strategies to manage intrusive cognitions would be differentially reflected in psychophysiological measures (i.e., error-related negativity) of individuals characterized by obsessive-compulsive symptoms -a group commonly associated with suppression efforts- relative to a low OC control. 67 participants with high and low OC symptoms were randomly assigned to cognitive strategy (suppression or acceptance). Participants watched an emotion-eliciting video clip and used the assigned cognitive strategy while performing the Stroop task. EEG data was collected. Consistent with well-established and emerging literature, ERN was enhanced in individuals with high OC symptoms and a marginal effect of thought-control strategy was observed, such that ERN amplitude was reduced in the suppression condition and greater for the acceptance condition. Uniquely, the study expanded on emerging literature by exploring whether the relationship between ERN and cognitive strategies was moderated by OC level. Although results were not conclusive, these preliminary findings represent an important first step to study effects of suppression and acceptance on the ERN in a sample characterized by clinically-relevant symptoms and overall encourage further exploration

Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography

The positional relationships among all of the visible organelles in a densely packed region of cytoplasm from an insulin secreting, cultured mammalian cell have been analyzed in three dimensions (3-D) at ≈6 nm resolution. Part of a fast frozen/freeze-substituted HIT-T15 cell that included a large portion of the Golgi ribbon was reconstructed in 3-D by electron tomography. The reconstructed volume (3.1 × 3.2 × 1.2 μm(3)) allowed sites of interaction between organelles, and between microtubules and organellar membranes, to be accurately defined in 3-D and quantitatively analyzed by spatial density analyses. Our data confirm that the Golgi in an interphase mammalian cell is a single, ribbon-like organelle composed of stacks of flattened cisternae punctuated by openings of various sizes [Rambourg, A., Clermont, Y., & Hermo, L. (1979) Am. J. Anat. 154, 455–476]. The data also show that the endoplasmic reticulum (ER) is a single continuous compartment that forms close contacts with mitochondria, multiple trans Golgi cisternae, and compartments of the endo-lysosomal system. This ER traverses the Golgi ribbon from one side to the other via cisternal openings. Microtubules form close, non-random associations with the cis Golgi, the ER, and endo-lysosomal compartments. Despite the dense packing of organelles in this Golgi region, ≈66% of the reconstructed volume is calculated to represent cytoplasmic matrix. We relate the intimacy of structural associations between organelles in the Golgi region, as quantified by spatial density analyses, to biochemical mechanisms for membrane trafficking and organellar communication in mammalian cells