314 research outputs found
Inferring and perturbing cell fate regulomes in human brain organoids
Self-organizing neural organoids grown from pluripotent stem cells(1-3) combined with single-cell genomic technologies provide opportunities to examine gene regulatory networks underlying human brain development. Here we acquire single-cell transcriptome and accessible chromatin data over a dense time course in human organoids covering neuroepithelial formation, patterning, brain regionalization and neurogenesis, and identify temporally dynamic and brain-region-specific regulatory regions. We developed Pando-a flexible framework that incorporates multi-omic data and predictions of transcription-factor-binding sites to infer a global gene regulatory network describing organoid development. We use pooled genetic perturbation with single-cell transcriptome readout to assess transcription factor requirement for cell fate and state regulation in organoids. We find that certain factors regulate the abundance of cell fates, whereas other factors affect neuronal cell states after differentiation. We show that the transcription factor GLI3 is required for cortical fate establishment in humans, recapitulating previous research performed in mammalian model systems. We measure transcriptome and chromatin accessibility in normal or GLI3-perturbed cells and identify two distinct GLI3 regulomes that are central to telencephalic fate decisions: one regulating dorsoventral patterning with HES4/5 as direct GLI3 targets, and one controlling ganglionic eminence diversification later in development. Together, we provide a framework for how human model systems and single-cell technologies can be leveraged to reconstruct human developmental biology
Durable remissions in TCF3-HLF positive acute lymphoblastic leukemia with blinatumomab and stem cell transplantation
First study of \eta_c, \eta(1760) and X(1835) production via \eta'\pi^+\pi^- final states in two-photon collisions
The invariant mass spectrum of the \eta' \pi^+ \pi^- final state produced in
two-photon collisions is obtained using a 673 fb^{-1} data sample collected in
the vicinity of the \Upsilon(4S) resonance with the Belle detector at the KEKB
asymmetric-energy e^+e^- collider. We observe a clear signal of the \eta_c and
measure its mass and width to be M(\eta_c)=(2982.7 +- 1.8(stat) +- 2.2(syst) +-
0.3(model)) MeV/c^2 and \Gamma(\eta_c) = (37.8^{+5.8}_{-5.3}(stat) +- 2.8(syst)
+- 1.4(model)) MeV/c^2. The third error is an uncertainty due to possible
interference between the \eta_c and a non-resonant component. We also report
the first evidence for \eta(1760) decay to \eta' \pi^+ \pi^-; we find two
solutions for its parameters, depending on the inclusion or not of the X(1835),
whose existence is of marginal significance in our data. From a fit to the mass
spectrum using coherent X(1835) and \eta(1760) resonant amplitudes, we set a
90% confidence level upper limit on the product \Gamma_{\gamma\gamma} \BR
(\eta' \pi^+ \pi^-) for the X(1835).Comment: 13 pages, 7 figures, submitted to PR
Evidence for B- -> Ds+ K- l- nubar and search for B- -> Ds*+ K- l- nubar
We report measurements of the decays B- -> Ds(*)+ K- l- nubar in a data
sample containing 657x10^6 BBbar pairs collected with the Belle detector at the
KEKB asymmetric-energy e+e- collider. We observe a signal with a significance
of 6 sigma for the combined Ds and Ds* modes and find the first evidence of the
B- -> Ds+ K- l- nubar decay with a significance of 3.4 sigma. We measure the
following branching fractions: BF(B- -> Ds+ K- l nubar) = (0.30 +/- 0.09(stat)
+0.11 -0.08(syst)) x 10^-3 and BF(B- -> Ds*+ K- l- nubar) = (0.59 +/-
0.12(stat) +/- 0.15(syst)) x 10^-3 and set an upper limit BF(B- -> Ds*+ K- l-
nubar) < 0.56 x 10^-3 at the 90% confidence level. We also present the first
measurement of the Ds+K- invariant mass distribution in these decays, which is
dominated by a prominent peak around 2.6 GeV/c^2.Comment: Submitted to Phys. Rev.
Measurements of the masses and widths of the and baryons
We present measurements of the masses and decay widths of the baryonic states
and using a data sample
corresponding to an integrated luminosity of 711 fb collected with the
Belle detector at the KEKB asymmetric-energy collider operating at
the resonance. We report the mass differences with respect to
the baryon MeV/, MeV/,
MeV/, MeV/, and the decay widths
MeV/,
MeV/,
MeV/,
MeV/,
where the first uncertainties are statistical and the second are systematic.
The isospin mass splittings are measured to be
MeV/ and
MeV/. These results are the most precise to date.Comment: 13 pages, 4 figures, Submitted to PRD(RC
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