Isolation, characterization and optimization of bacterial isolate SARR1 for biodegradation of pretreated low density polyethylene

Accumulation of low-density polyethylene (LDPE) has caused a threat to the environment because of its stable and inert nature as it cannot be degraded easily by microorganisms. Its lightweight, low cost, strength, durability, and its various other applications, have led to the wide usage of the polymer, which is exerting a negative effect on both marine and land biota. The development of an eco-friendly or a promising strategy is needed to reduce LDPE waste from both land and water. In the present study, observations have been made to isolate highly efficient LDPE degrading bacteria. The response surface methodology (RSM) was used to predict the best optimization of media for the degradation of LDPE by isolate SARR1. The isolate SARR1 was selected through primary screening by weight loss method and secondary screening using CO2 evolution test, TTC and MATH Test. The isolate SARR1 showed 6.30 ± 0.25 g/L CO2 evolution. The microbial adhesion hydrophobicity (MATH) was observed during log phase (100 to 56.89 ± 0.97 %) and stationary phase (100 to 82.92 ± 1.24 %). An isolate SARR1 converted the TTC into red coloured insoluble triphenyl formazan (TPF) after incubation of 7 days. The isolated bacteria SARR1 showed 38.3 ± 1.27 % biodegradation efficiency in the pretreated LDPE strips at 37 °C and pH 7.0 under optimized conditions within 30 days of incubation. This bioremediation and biodegradation approach is eco-friendly and safe for the environment. The results of treatment with isolate SARR1 had a potential hope to degrade LDPE at higher rate than natural degradation. 

Seed storage protein phylogenetics of Indian wheat genotypes belong to Triticum aestivum, T. dicoccum and T. durum

17-27Genetic diversity analysis of eighty six Indian wheat genotypes was performed using sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) electrophoresis based on their total seed storage protein. A dendrogram was constructed using the genetic similarity coefficient matrix based on SDS-PAGE, which categorized these genotypes according to their electrophoretic patterns into eight different clusters. These eighty six genotypes studied here belong to three different species of wheat. Out of these eighty six genotypes, two genotypes belong to T. dicoccum; eleven belong to T. durum and the remaining seventy three belongs to T. aestivum. In the dendrogram, all the eleven genotypes of the species T. durum (A-9-30-1, HD-4672, RAJ-1555, PDW-233, PBW-34, HI-8381, PDW-291, WH-896, PDW-215, HI-8498 and MACS-2846) were grouped in one cluster. The pedigree analysis of genotypes showed similar parentage with the electrophoretic profiling of the different wheat genotypes. From this study, it has been concluded that the total seed storage protein profile of wheat can be used as a marker for genetic diversity

Phytoremediation technologies and their mechanism for removal of heavy metal from contaminated soil: An approach for a sustainable environment

The contamination of soils with heavy metals and its associated hazardous effects are a thrust area of today’s research. Rapid industrialization, emissions from automobiles, agricultural inputs, improper disposal of waste, etc., are the major causes of soil contamination with heavy metals. These contaminants not only contaminate soil but also groundwater, reducing agricultural land and hence food quality. These contaminants enter the food chain and have a severe effect on human health. It is important to remove these contaminants from the soil. Various economic and ecological strategies are required to restore the soils contaminated with heavy metals. Phytoremediation is an emerging technology that is non-invasive, cost-effective, and aesthetically pleasing. Many metal-binding proteins (MBPs) of the plants are significantly involved in the phytoremediation of heavy metals; the MBPs include metallothioneins; phytochelatins; metalloenzymes; metal-activated enzymes; and many metal storage proteins, carrier proteins, and channel proteins. Plants are genetically modified to enhance their phytoremediation capacity. In Arabidopsis, the expression of the mercuric ion-binding protein in Bacillus megaterium improves the metal accumulation capacity. The phytoremediation efficiency of plants is also enhanced when assisted with microorganisms, biochar, and/or chemicals. Removing heavy metals from agricultural land without challenging food security is almost impossible. As a result, crop selections with the ability to sequester heavy metals and provide food security are in high demand. This paper summarizes the role of plant proteins and plant–microbe interaction in remediating soils contaminated with heavy metals. Biotechnological approaches or genetic engineering can also be used to tackle the problem of heavy metal contamination