Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense

Microscopic and lectin histochemical studies were performed using the cerebella of 33 natural cases of Solanum fastigiatum var. fastigiatum intoxication in the cattle from southern Brazil and 2 natural and 4 experimental cases of Solanum bonariense from Uruguay. The following biotinylated lectins were used in both cases: WGA, sWGA, BS-I, Con-A, RCA-I, DBA, and UEA-I, with the addition of LCA in S. fastigiatum poisoning cases. Histologically, the lesions consisted of fine vacuolization, distention of portions of the Purkinje cells, axonal spheroids measuring 14-50 m in the granular cell layer and adjacent white matter and, proliferation of the Bergmann?s glia. Lectin histochemistry revealed strong reactivity of stored material in Purkinje neurons with the lectins sWGA, Con-A, and LCA in S. fastigiatum cases. A similar pattern was found in S. bonariense cases with a most intense reactions to WGA, and less intense reaction to Con-A, whereas BS-I and RCA-I binding was absent to poor in these neurons in all the cases studied.Lectin reactivity in Purkinje cells between cases was independent of cell damage (from mild to severe loss of neurons). Both S. fastigiatum and S. bonariense have similar lectin binding,  suggesting a similar pathogenesis. Since comparable binding patterns have been described in animals poisoned with swainsonine-containing plants, perhaps the toxins in these plants contain related glycosidaseinhibiting toxins or inhibit glycoprotein and lysosomal metabolism through some related mechanism. The results of this study showed that in spontaneous poisoning by S. fastigiatum and S. bonariense in the cattle, the pattern of lectin binding is similar to those observed in S. fastigiatum experimental conditions.Fil: Sant’Ana, Fabiano J.F.. Universidade Federal de Santa Catarina; BrasilFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Nishida, Fabian. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Verdes, José M.. Universidad de la República. Facultad de Ciencias; UruguayFil: Moraña, Antonio. Universidad de la República; UruguayFil: Barros, Claudio S.L.. Universidad de la República; Urugua

Multi-segmented maxillary ortho-surgical treatment in Class II patients with maxillary protrusion and open bite: a concise systematic review

Introduction: Anterior open bite is the lack of vertical contact or negative overbite between the anterior teeth of the upper and lower arches when the posterior teeth are in occlusion. Its etiology is multifactorial, including heredity, oral habits, unfavorable growth patterns, and increased lymphatic tissue along with mouth breathing and functional oral matrices. The characteristics of individuals with anterior open bite include excessive gonial, mandibular, and occlusal plane angles, mandibular small body, and ramus increased lower anterior facial height, decreased upper anterior facial height, retrusive jaw, Class II tendency, divergent cephalometric planes, lingual position, and inadequate lip seal. Objective: To carry out a concise systematic review of multi-segmented maxillary ortho-surgical treatment in class II patients with maxillary protrusion and open bite. Methods: Experimental and clinical studies (case reports, retrospective, prospective and randomized) with qualitative and/or quantitative analysis were included, following the rules of the systematic review-PRISMA. A total of 289 articles were initially found and, after selection, 17 articles were used to compose this study. Results and conclusion: Significant improvement in anterior occlusion can be expected in most patients when maxillary or mandibular surgery is used for Class II open bite correction. However, there will be individual patients in whom there will be considerable post-treatment changes in the anteroposterior and vertical dimensions. Although individual morphology needs to be taken into account, it appears that both short-term and long-term stability are likely to be greater after Le Fort I surgery compared to bilateral sagittal split osteotomy

Orthognathic surgery in the mandibular advance: a systematic review

Introduction: Orthognathic surgery is a standardized procedure used to improve a patient's facial appearance and to correct maxillary and mandibular deformities resulting from malocclusions, disease or trauma. Bilateral sagittal osteotomy of the mandibular ramus is a technique widely used in orthognathic surgery to correct mandibular deformities. Mandibular advancement is a procedure with a high risk of skeletal recurrence, due to the difference between the proximal and distal bone segments. Objective: To carry out a systematic review of orthognathic surgery in the mandibular advance, focusing on the main challenges and importance. Methods: The present study was followed by a systematic literature review model - PRISMA rules. The quality of the studies was based on the GRADE instrument. The risk of bias was analyzed according to the Cochrane instrument. Results and Conclusion: Bilateral sagittal osteotomy is the most used technique in mandibular orthognathic surgery, allowing mandibular movements in the sagittal, vertical and transverse directions, with good results and few complications. However, this technique can cause mandibular advancements above 10 mm, strongly pointing to the placement of a bone graft in order to avoid defects and their complications. Therefore, in orthognathic surgery, bone grafting can accelerate bone formation

Ortho-surgical procedure involving mandible, maxilla, mento and associations: a systematic review

Introduction: Orthognathic surgery (OS) consists of the surgical procedure that aims to correct deformities of the bones of the maxilla and mandible. The records of the first surgeries for the correction of dentofacial deformities date from the mid-nineteenth century and were initially limited to mandibular surgeries. The malocclusion has as one of the primary etiological factors the facial growth pattern. Angle's statement already said that the only possibility of correcting true dentofacial deformities was the combination of orthodontics with surgery, and the importance of the combined effort of these two distinct areas in the approach to dentofacial disharmony in patients with problems was recognized skeletal. Objective: This study aimed to review the literature on OS involving mandible, maxilla, mento and associations. Methods: Clinical studies with qualitative and/or quantitative analysis were included, following the rules of the systematic review-PRISMA. Results: A total of 107 articles were found involving “orthognathic surgery". A total of 47 articles were evaluated in full, and 33 were included and discussed in this study. Individuals with class III dentofacial deformities are those that normally present greater aesthetic and functional impact, and for this reason, are the ones that more frequently seek treatment. However, some studies have shown a tendency for complications to occur in older patients submitted to orthognathic surgeries. Mandibular surgical procedures with maxillary segmentation and combining three types of osteotomies should be carefully planned and trained to reduce the occurrence of complications. The surgeon, orthodontist, and all staff involved should be focused on avoiding complications during all phases of treatment. Conclusion: It was concluded that there was an increase in the cases of OS in the last years, and with homogeneous samples between the masculine and feminine genres, and the advances in maxillary surgery corresponded to the greater number of surgical treatments

Ortho-surgical treatment in Class II skeletal patients with mandibular retrognathism: a concise systematic review

Introduction: In the setting of class II malocclusion corrections, the union of orthodontic and surgical procedures was developed. Bilateral sagittal split osteotomy is standard protocol for correcting mandibular retrognathism. Class II dental malocclusion with deep bite needs to be treated early by orthodontics. Thus, the commonly used technique combines BSSO for mandibular advancement and recoil genioplasty to correct the resulting chin protrusion. Objective: To carry out a systematic review of the main considerations of ortho-surgical treatments in class II patients with mandibular retrognathism. Methods: Experimental and clinical studies (case reports, retrospective, prospective and randomized) with qualitative and/or quantitative analysis were included, following the rules of the systematic review-PRISMA. Results and Conclusion: A total of 128 articles was found involving class II malocclusion and ortho-surgical treatments. After, a total of 64 articles were fully evaluated and 24 were included and discussed in this study. A meta-analysis study evaluated the best functional appliance improving mandibular length in individuals with retrognathism. Sander Bite Jumping reported the greatest increase in mandibular length, with 3.40 mm. Another meta-analysis study compared dental, skeletal, and aesthetic outcomes between orthodontic camouflage and orthodontic-surgical treatment in patients with Class II skeletal malocclusion and retrognathic mandible with anterior growth. The difference between treatments was not statistically significant regarding SNA angle, linear measure of the lower lip to the Ricketts aesthetic line, convexity of the skeletal profile or soft tissue profile excluding the nose. In contrast, orthodontic-surgical treatment was more effective to the ANB, SNB and ML/NSL angles and the soft tissue profile including the nose

Main approaches of three-dimensional (3D) imaging examinations in the assessment, treatment, and follow-up of patients with skeletal Class III: a concise systematic review

Introduction: Class III malocclusion presents with several skeletal and dental factors, highlighting the large or protruding jaw, retrusive maxilla, protrusive mandibular dentition, retrusive maxillary dentition, or combinations. The importance of evaluating class III malocclusions, as well as their treatments, through three-dimensional (3D) images are highlighted. Objective: To present a concise systematic review of the approach of 3D imaging exams to conduct class III malocclusion treatments, as well as to follow up after orthognathic surgery. Methods: Clinical studies with qualitative and/or quantitative analysis were included, following the rules of the systematic review-PRISMA. Results and Conclusion: A total of 105 articles was found involving class III malocclusion and ortho-surgical treatments guided by three-dimensional (3D) images. A total of 44 articles were fully evaluated and 16 were included and discussed in this study. Positional, structural, and volumetric condylar changes after orthognathic surgery in skeletal Class III patients have been well elucidated using cone-beam computed tomography. In addition, the virtual surgical planning and the surgical splint manufactured in CAD/CAM facilitate treatment planning and offer an accurate surgical result in orthognathic surgery. The application of pre-engineered bone guidance splints allows the treatment of craniofacial deformities with precision. Therefore, the three-dimensional (3D) tool allows for the accuracy of treatments and planning of orthognathic surgery, as well as the best postoperative follow-up

Parâmetros hematológicos e bioquímicos de ratos submetidos à dieta hipoprotéica e hipercalórica

A má-nutrição é um problema de saúde pública que ainda assola crianças e adultos no mundo inteiro, principalmente em países em desenvolvimento. A carência de proteínas, causando Kwashiorkor, é o tipo de má-nutrição mais prevalente, pois fontes de alimentos protéicos, geralmente, são mais onerosas. Uma dieta hipoprotéica causa alterações metabólicas num animal em intensidades diretamente proporcionais ao nível de depleção de proteínas, bem como o tempo em que o indivíduo permanece sob o estado de subnutrição. Nesse sentido, propõe-se avaliar a severidade de uma dieta hipoprotéica a 4% para ratos jovens. Utilizam-se 30 ratos Wistar (90 dias de idade), divididos em grupo controle (15) e experimental (15). O GC recebeu dieta nor0moprotéica, enquanto o GE recebeu dieta com 4% de teor de proteínas, ambos durante 12 semanas. No fi nal do experimento, sangue foi coletado para realização de hemograma e dosagem de atividade de fosfatase alcalina, alanina aminotrasferase, além da concentração de proteínas totais e frações, colesterol total, triglicerídeos, uréia, ácido úrico, T3, T4 e aminoácidos plasmáticos. Os animais do GE demonstraram menor atividade defosfatase alcalina no sangue, anemia microcítica normocrômica, hipoproteinemia, hipoglobulinemia, reduação na concentração plasmática de triglicerídeos, aumento da concentração plasmática de T3 e T4 e diminuição da concentração plasmática dos seguintes aminoácidos: metionina, fenilalanina, valina, leucina e isoleucina

THE MOTIVATIONS FOR THE USE OF ILLICIT SUBSTANCES: A STUDY IN A THERAPEUTIC COMMUNITY IN MANAUS, BRAZIL

ABSTRACT: The abuse of illicit drugs has become a public health problem in Brazil. Anti-drug laws were instituted with the intention of minimizing drug trafficking and, consequently, the use of these substances. The objective of this study was to identify the motivations that led them to the use of illicit substances in a group of male residents of a therapeutic community in Manaus. This work corresponds to a qualitative and exploratory research. The access to the field was through the possession of the Term of Consent, made available by the Fazenda da Esperança therapeutic community.  Five men residents agreed to participate. For data collection, a semi-structured interview was used, which followed a previously elaborated script, which contained key questions for discussion and treatment, and data analysis, using Bardin's Content Analysis technique. The present study proved that the most critical factors as motivation for substance use are colleagues and family conflict. It is well established that friends who use drugs are more likely to use drugs. From the interviewees' statements, it was found that friends not only provide immediate access to substances, but also shape drug use behavior and help shape positive beliefs and attitudes toward drug use. Substance use by friends is also likely to influence perceptions of how normative substance use is among peers.   Keywords: Drugs; Therapeutic Community; Treatment.   RESUMO: O consumo abusivo de drogas ilícitas tornou-se um problema de saúde pública no Brasil. Leis e decretos “antidrogas” foram instituídos com a intenção de minimizar o tráfico destas e, consequentemente, a utilização dessas substâncias. A partir disso, o objetivo deste trabalho foi identificar as motivações que os levaram ao uso de substâncias ilícitas em um grupo de residentes do gênero masculino de uma comunidade terapêutica em Manaus. Este trabalho corresponde a uma pesquisa qualitativa com caráter exploratório. O acesso ao campo se deu através da posse do Termo de Anuência, disponibilizado pela comunidade terapêutica Fazenda da Esperança e ao adentrar a comunidade, a pesquisadora se fez presente nos espaços de convivência para o convite dos possíveis participantes, que no total, foram cinco homens residentes da comunidade.   Para a coleta dos dados, foi utilizada a entrevista semiestruturada, que seguiu um roteiro previamente elaborado, que continha questões chave para discussão e para tratamento e a análise de dados, foi utilizado a técnica de Análise de Conteúdo, de Bardin. O presente estudo comprovou que os fatores mais importantes como motivação para o uso de substâncias estão os colegas, o conflito familiar. De fato, está bem estabelecido que têm amigos que usam drogas têm maior probabilidade de usar drogas. Verificou-se, pelas falas dos entrevistados, que os amigos não apenas fornecem acesso imediato a substâncias, mas também modelam o comportamento de uso de drogas e ajudam a moldar crenças e atitudes positivas em relação ao uso de drogas. O uso de substâncias dos amigos também provavelmente influenciará as percepções de como o uso normativo de substâncias é entre os pares.   Palavras-chave: Drogas; Comunidade Terapêutica; Tratamento