161 research outputs found
Tecnologia de produção de vinagre de mel.
Com a perspectiva de diversificação dos produtos derivados do mel, este trabalho teve como finalidade avaliar a produção de vinagre de mel de abelhas africanizadas (Apis mellifera L.). A produção do vinagre foi realizada através da fermentação acética de hidromel com teor alcoólico de 8% (v/v), obtido a partir de um mosto com 17,11% (m/v) de açúcares totais. A fermentação acética foi realizada pelo método rápido, em fermentador vertical com capacidade de 15 litros, utilizando vinagre forte não pasteurizado como inóculo, por 72 horas. Durante esta fermentação foram monitoradas as temperaturas interna (fermentador) e ambiente. O vinagre obtido apresentou acidez em torno de 9% e teor alcoólico em torno de 1% (v/v). O rendimento da fermentação acética foi entre 91,2 e 97,17%. O produto final foi diluído de acordo com a legislação brasileira (4,0% de acidez) e analisado sensorialmente, demonstrando ser de boa aceitabilidade Os teores de álcool etílico, ácido acético, resíduo mineral fixo e resíduo seco a 105oC foram de 0,32%; 4,2%; 0,05% e 1,76%, respectivamente. A análise microscópica revelou ausência de sujidades, larvas e parasitas.bitstream/CPAP-2009-09/57063/1/BP86.pd
Incorporation of proteins in large pore SBA-15.
Ordered mesoporous silica (OMS) SBA-15 has morphological properties useful for many applications, including high surface area, hydrothermal mechanical stability and selective adjustable pore size. These properties make the SBA-15 interesting for many applications such as catalysis, big molecules selection and protein encapsulation. Usually, a pore diameter of 10 to 12 nm is obtained in a conventional synthesis process with Pluronic 123 template
Inhibition of human transthyretin aggregation by non-steroidal anti-inflammatory compounds: a structural and thermodynamic analysis
Transthyretin (TTR) is a homotetrameric protein that circulates in plasma and cerebral spinal fluid (CSF) whose aggregation into amyloid fibrils has been associated with at least two different amyloid diseases: senile systemic amyloidosis (SSA) and familial amyloid polyneuropathy (FAP). In SSA aggregates are composed of WT-TTR, while in FAP more than 100 already-described variants have been found in deposits. Until now, TTR-related diseases have been untreatable, although a new drug called Tafamidis has been approved only in Europe to specifically treat V30M patients. Thus, new strategies are still necessary to treat FAP caused by other variants of TTR. TTR has two channels in the dimer interface that bind to the hormone thyroxin and that have been used to accommodate anti-amyloidogenic compounds. These compounds stabilize the tetramers, rendering TTR less amyloidogenic. Here, we investigated the effects of three non-steroidal anti-inflammatory compounds-sulindac (SUL), indomethacin (IND) and lumiracoxib (LUM)-as tetramer stabilizers and aggregation inhibitors. WT-TTR and the very aggressive TTR variant L55P were used as models. These compounds were able to stabilize TTR against high hydrostatic pressure (HHP), increasing the ΔGf by several kcal. They were also effective in inhibiting WT-TTR and L55P acid- or HHP-induced aggregation; in particular, LUM and IND were very effective, inhibiting almost 100% of the aggregation of both proteins under certain conditions. The species formed when aggregation was performed in the presence of these compounds were much less toxic to cells in culture. The crystal structures of WT-TTR bound to the three compounds were solved at high resolution, allowing the identification of the relevant protein:drug interactions. We discuss here the ligand-binding features of LUM, IND and SUL to TTR, emphasizing the critical interactions that render the protein more stable and less amyloidogenic.CAPESCNPqInstituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem (INBEB)FAPERJFAPES
Corrigendum: Quantifying the Responses of Three Bacillus cereus Strains in Isothermal Conditions and During Spray Drying of Different Carrier Agents
Parasite and egg burden, hepatic collagen and histologic pattern of liver granulomas in selection III high and low antibody responder mice infected with Schistosoma mansoni
Coleta de lavado gástrico para diagnóstico de tuberculose pulmonar infantil: revisão sistemática
Depression and sickness behavior are Janus-faced responses to shared inflammatory pathways
It is of considerable translational importance whether depression is a form or a consequence of sickness behavior. Sickness behavior is a behavioral complex induced by infections and immune trauma and mediated by pro-inflammatory cytokines. It is an adaptive response that enhances recovery by conserving energy to combat acute inflammation. There are considerable phenomenological similarities between sickness behavior and depression, for example, behavioral inhibition, anorexia and weight loss, and melancholic (anhedonia), physio-somatic (fatigue, hyperalgesia, malaise), anxiety and neurocognitive symptoms. In clinical depression, however, a transition occurs to sensitization of immuno-inflammatory pathways, progressive damage by oxidative and nitrosative stress to lipids, proteins, and DNA, and autoimmune responses directed against self-epitopes. The latter mechanisms are the substrate of a neuroprogressive process, whereby multiple depressive episodes cause neural tissue damage and consequent functional and cognitive sequelae. Thus, shared immuno-inflammatory pathways underpin the physiology of sickness behavior and the pathophysiology of clinical depression explaining their partially overlapping phenomenology. Inflammation may provoke a Janus-faced response with a good, acute side, generating protective inflammation through sickness behavior and a bad, chronic side, for example, clinical depression, a lifelong disorder with positive feedback loops between (neuro)inflammation and (neuro)degenerative processes following less well defined triggers
Administration of M. leprae Hsp65 Interferes with the Murine Lupus Progression
The heat shock protein [Hsp] family guides several steps during protein synthesis, are abundant in prokaryotic and eukaryotic cells, and are highly conserved during evolution. The Hsp60 family is involved in assembly and transport of proteins, and is expressed at very high levels during autoimmunity or autoinflammatory phenomena. Here, the pathophysiological role of the wild type [WT] and the point mutated K409A recombinant Hsp65 of M. leprae in an animal model of Systemic Lupus Erythematosus [SLE] was evaluated in vivo using the genetically homogeneous [NZBxNZW]F1 mice. Anti-DNA and anti-Hsp65 antibodies responsiveness was individually measured during the animal's life span, and the mean survival time [MST] was determined. The treatment with WT abbreviates the MST in 46%, when compared to non-treated mice [p<0.001]. An increase in the IgG2a/IgG1 anti-DNA antibodies ratio was also observed in animals injected with the WT Hsp65. Incubation of BALB/c macrophages with F1 serum from WT treated mice resulted in acute cell necrosis; treatment of these cells with serum from K409A treated mice did not cause any toxic effect. Moreover, the involvement of WT correlates with age and is dose-dependent. Our data suggest that Hsp65 may be a central molecule intervening in the progression of the SLE, and that the point mutated K409A recombinant immunogenic molecule, that counteracts the deleterious effect of WT, may act mitigating and delaying the development of SLE in treated mice. This study gives new insights into the general biological role of Hsp and the significant impact of environmental factors during the pathogenesis of this autoimmune process
Os limites da abordagem clássica dos acidentes de trabalho: o caso do setor extrativista vegetal em Minas Gerais
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