Lactate dehydrogenase as a marker of Plasmodium infection in malaria vector Anopheles

Lactate dehydrogenase (Ldh) electrophoresis showed the presence of #Plasmodium yoelii yoelii in #Anopheles stephensi and #An. gambiae. The Ldh appeared as an additional band (pLdh) whose activity was more intense with 3-acetyl pyridine adenine dinucleotide as coenzyme than with beta nicotinamide adenine dinucleotide. Several allelic forms occurred both in the vector and the host. The isoelectric point of Ldh, similar in the vector and host, differed from those of Ldh from mosquito and mouse. The presence of pLdh was detected from the 2nd to the 28th day of infection. The pLdh appeared to be proportional to the number of sporozoites present in infected salivary glands. However, pLdh was not found in salivary glands or midguts, but it was detected in the rest of the corresponding mosquito. The origin and use of pLdh as a marker of #Plasmodium in its vector is discussed. (Résumé d'auteur

Specific duplication and dorsoventrally asymmetric expression patterns of Cycloidea-like genes in zygomorphic species of Ranunculaceae.

Floral bilateral symmetry (zygomorphy) has evolved several times independently in angiosperms from radially symmetrical (actinomorphic) ancestral states. Homologs of the Antirrhinum majus Cycloidea gene (Cyc) have been shown to control floral symmetry in diverse groups in core eudicots. In the basal eudicot family Ranunculaceae, there is a single evolutionary transition from actinomorphy to zygomorphy in the stem lineage of the tribe Delphinieae. We characterized Cyc homologs in 18 genera of Ranunculaceae, including the four genera of Delphinieae, in a sampling that represents the floral morphological diversity of this tribe, and reconstructed the evolutionary history of this gene family in Ranunculaceae. Within each of the two RanaCyL (Ranunculaceae Cycloidea-like) lineages previously identified, an additional duplication possibly predating the emergence of the Delphinieae was found, resulting in up to four gene copies in zygomorphic species. Expression analyses indicate that the RanaCyL paralogs are expressed early in floral buds and that the duration of their expression varies between species and paralog class. At most one RanaCyL paralog was expressed during the late stages of floral development in the actinomorphic species studied whereas all paralogs from the zygomorphic species were expressed, composing a species-specific identity code for perianth organs. The contrasted asymmetric patterns of expression observed in the two zygomorphic species is discussed in relation to their distinct perianth architecture

Spontaneous HIV expression during suppressive ART is associated with the magnitude and function of HIV-specific CD4+ and CD8+ T cells.

Spontaneous transcription and translation of HIV can persist during suppressive antiretroviral therapy (ART). The quantity, phenotype, and biological relevance of this spontaneously "active" reservoir remain unclear. Using multiplexed single-cell RNAflow-fluorescence in situ hybridization (FISH), we detect active HIV transcription in 14/18 people with HIV on suppressive ART, with a median of 28/million CD4 <sup>+</sup> T cells. While these cells predominantly exhibit abortive transcription, p24-expressing cells are evident in 39% of participants. Phenotypically diverse, active reservoirs are enriched in central memory T cells and CCR6- and activation-marker-expressing cells. The magnitude of the active reservoir positively correlates with total HIV-specific CD4 <sup>+</sup> and CD8 <sup>+</sup> T cell responses and with multiple HIV-specific T cell clusters identified by unsupervised analysis. These associations are particularly strong with p24-expressing active reservoir cells. Single-cell vDNA sequencing shows that active reservoirs are largely dominated by defective proviruses. Our data suggest that these reservoirs maintain HIV-specific CD4 <sup>+</sup> and CD8 <sup>+</sup> T responses during suppressive ART

Sustained IFN signaling is associated with delayed development of SARS-CoV-2-specific immunity.

Plasma RNAemia, delayed antibody responses and inflammation predict COVID-19 outcomes, but the mechanisms underlying these immunovirological patterns are poorly understood. We profile 782 longitudinal plasma samples from 318 hospitalized patients with COVID-19. Integrated analysis using k-means reveals four patient clusters in a discovery cohort: mechanically ventilated critically-ill cases are subdivided into good prognosis and high-fatality clusters (reproduced in a validation cohort), while non-critical survivors segregate into high and low early antibody responders. Only the high-fatality cluster is enriched for transcriptomic signatures associated with COVID-19 severity, and each cluster has distinct RBD-specific antibody elicitation kinetics. Both critical and non-critical clusters with delayed antibody responses exhibit sustained IFN signatures, which negatively correlate with contemporaneous RBD-specific IgG levels and absolute SARS-CoV-2-specific B and CD4 <sup>+</sup> T cell frequencies. These data suggest that the "Interferon paradox" previously described in murine LCMV models is operative in COVID-19, with excessive IFN signaling delaying development of adaptive virus-specific immunity

Socio-economic assessement of farmers' vulnerability as water users subject to global change stressors in the hard rock area of southern India. The SHIVA ANR project

International audienceDemand for vulnerability assessments is growing in policy-making circles, to support the choice of appropriate measures and policies to reduce the vulnerability of water users and resources. Through the SHIVA ANR project, we are seeking a method to assess and map the vulnerability of farmers in southern India to both climate and socioeconomic changes, and secondly, to assess the costs and benefits associated with trends farmers' vulnerability in the medium and long-term. The project is focusing on southern India 's hard rock area, as in the geological context, both surface and ground water resources are naturally limited. We are also focusing on farming populations as these are the main water users in the area and rely exclusively on groundwater. The area covers southern India's semi-arid zone, where the rainfall gradient ranges from 600 mm to 1100 mm. Vulnerability is expected to vary according to local climatic conditions but also the socioeconomic characteristics of farming households. The SHIVA research team has been divided into six thematic groups in order to address the different scientific issues : downscaling the regional climate scenario, farm area projections, vulnerability assessments and quantification, vulnerability mapping, hydrological modelling and upscaling, and vulnerability impact assessements. Our approach is multidisciplinary to cater for for numerous inherent themes, and integrated to cater for vulnerability as a dynamic and multidimensional concept. The project 's first results after 10 months of research are presented below

An interactive interface for directing virtual humans

Research on virtual humans spans from body animation to speech generation. In many cases research systems are isolated software pieces and can only be used after a steep learning curve. A novel system is presented which integrates a large repertoire of virtual human research into one piece of user-friendly software, the virtual human director (VHD). This software achieves two important goals without any decrease in real-time performance or graphics quality. One goal is the integration of all the existing real-time virtual human technology from two research laboratories into open software architecture. The other one is to develop an intuitive user interface, which allows artists and directors to work with virtual human