Impact of black plum (Vitex doniana) leaf meal on blood biochemistry, hormone and cholesterol level of West African dwarf goat-bucks

Fifteen (15) West African Dwarf (WAD) goat-bucks with average initial weights of 10±0.53kg were used in an experiment to investigate the effect of varying levels of Vitex doniana leaf meal on the blood profiles, hormone and cholesterol level of WAD goat-bucks. The animals were randomly assigned to five dietary treatments consisting of graded levels of Vitex doniana in a Completely Randomized Design experiment which lasted twenty-eight (28) days. At the end of the feeding trial, blood samples were collected from the jugular veins of the animal for the determination of haematological and serum biochemical characteristics using standard laboratory methods. The Vitex doniana leaf had a crude protein of 8.53, crude fibre of 7.15, crude fat of 6.44 and ash of 8.26 percent and were moderately high in flavonoids 20.82, phenols 96.14, saponin 6.48mg/100g with slight presence of alkaloids, tannins, terpenoids, steroids and anthraquinones. The effects of graded levels of Vitex doniana leaf meal on haematology of WAD goat-bucks reveals that red blood cell (RBC) significantly (p<0.05) increase with concurrent increase in Vitex doniana leaf meal whereas the haemoglobin (Hb) and packed cell volume (PCV) reduced with the addition of the leaf meal. Other parameters were not significantly (p>0.05) different from the control, although it varies with increase in the leaf meal addition. The serum biochemistry of West African dwarf bucks showed that total protein (TP), albumin (ALB), urea and glucose significantly increased with increase in Vitex doniana leaf meal. Alkaline phosphatase (ALP), Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) was significantly (p<0.05) influenced by the increase in Vitex doniana leaf meal inclusion. The ALT and AST were not significantly (p>0.05) different from the control in treatment D. Phytochemical results shows Vitex doniana has high content of phenol, flavonoid and saponin; it significantly increase total cholesterol and low density lipoprotein with a reduction in high density lipoprotein with 20g/kg feed while triglyceride and high density lipoprotein were high with 10g/kg feed. It significantly reduced luteinizing hormone while causing an increase in follicle stimulating hormone, testosterone and prolactin with 15g/kg. The administration of 15g inclusion level of Vitex doniana leaf meal increases the reproductive performance while administration of 10g inclusion level helped to lower the cholesterol level in WAD bucks with no conspicuous adverse effects and their health status was not compromised. All the haematological and serum biochemical characteristics of the bucks were within the normal/ standard blood ranges for apparently healthy bucks. The study therefore concludes that farmers can supplement feed with up to 20% Vitex doniana leaf meal in formulated diets meant for goats, without fear of compromising haematopoietic processes. Keywords: Blood, Feed, leaf-meal, WAD goat-buck

DNA from Plant leaf Extracts: A Review for Emerging and Promising Novel Green Corrosion Inhibitors.

With growing global awareness and concern for environmental protection through the use of less hazardous and environmentally-friendly extracts of plant origin, there has been a plethora of green corrosion inhibitors research with far reaching contributions to the science of corrosion prevention and control. Attention has increasingly turned towards green corrosion inhibitors, compounds of natural origin with anti-oxidant activity towards metals and their alloys. Green inhibitors have been investigated for their corrosion and adsorption properties with good results. The findings from these research works provide evidence of the adsorption behavior of green inhibitors which was confirmed by the adsorption isotherms that were proposed. Adsorption is the first step of any surface reaction and since corrosion is a surface phenomenon the effectiveness of green corrosion inhibitors is related to their ability to adsorb on metal surfaces. This review proposes the potential of plant dna as an emerging and promising novel inhibitor for mild steel. It begins with a list of plants that have been used in studies to determine corrosion inhibition properties and moves on to establish the adsorption behavior of bio macromolecules; protein, polysaccharides (chitosan) and dna. It reviews studies and investigation of dna interaction and adsorption on inorganic surfaces before focusing on the use of salmon (fish) sperm dna and calf thymus gland dna as green corrosion inhibitors for mild steel. It concludes that plant dna is a promising candidate for green corrosion inhibitor given the similarity between the plant and animal dna structure and function, and the fact that the use of plant is more environmentally sustainable than animal-based produc

Organ weight, serum biochemistry and gut microbial counts of Thryonomys swinderianus fed diet supplemented with Vernonia amygdalina leaf meal

The study investigated the effect of Vernonia amygdalina (VA) leaf supplementation on organ weight and gut microbial count of Thryonomys  swinderianus. Thirty-six weaner grasscutters of mixed sexes were allotted randomly to six treatments (A, B, C, D, E, and F) in a completely randomized design for a nutritional trial of 8 weeks. At the end of the experiment, caecum, kidney, liver, lungs, intestine and the heart were excised  to determine their weights and the intestinal/caecal contents and scrapings were obtained to determine the fungi and bacteria population. Data collected were analyzed using one-way Analysis of Variance (ANOVA). The VA supplementation had significant effect (p<0.05) on relative organs with a range of 0.47 –0.60%, 0.40 – 0.62%, 1.20 – 2.83%, 0.52 - 0.58%, 5.53 – 12.91%, 714.00 – 960.00g, 494.00 –781.00g and 479.00 – 747.00g for lungs, heart, liver, kidney, intestine, live weight, eviscerated and carcass weight of the grasscutter respectively, with a smaller relative weight of the kidney from 3g/kg VA supplementation upwards. The serum biochemical parameters were also significantly affected by VA supplementation. There was a  decrease in alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase but were within the normal range required for rodents. Total protein and Albumin were significantly improved by the supplement while total bilirubin was variable with a significant decrease prior to 4gVA supplementation. Microbial populations in the intestine and ceacum were reduced to varying extent by the supplement. It could be  concluded that VA supplementation reduced intestinal and caecal microbes and increased the live and carcass weights but decreased relative weights of selected organs in the grasscutter. Keywords: Grasscutter, Vernonia amygdalina, Organ weight, Microbial count

Flow of viscous fluid along a nonlinearly stretching curved surface

This paper focuses on the flow of viscous fluid over a curved surface stretching with nonlinear power-law velocity. The boundary layer equations are transformed into ordinary differential equations using suitable non-dimensional transformations. These equations are solved numerically using shooting and Runge-Kutta (RK) methods. The impact of non-dimensional radius of curvature and power-law indices on the velocity field, the pressure and the skin friction coefficient are investigated. The results deduced for linear stretching are compared with the published work to validate the numerical procedure. The important findings are: (a) Slight variation of the curvature of the stretching sheet increases the velocity and the skin friction coefficient significantly. (b) The nonlinearity of the stretching velocity increases the skin friction. (c) The results for linear stretching and the flat surface are the special cases of this problem. Keywords: Boundary layer flow, Curved surface, Nonlinearly stretching surface, Variable curvatur